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ITCH Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6137213

MBS6137213 is an antibody targeting ITCH. Also known as ITCH (E3 Ubiquitin-protein Ligase Itchy Homolog, Itch, Atrophin-1-interacting Protein 4, AIP4, NFE2-associated Polypeptide 1, NAPP1, DJ468O1.1) APC. Reactive in Human. Host: Mouse. More details

$1155 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ITCH, Monoclonal Antibody
Full Product Name
ITCH (E3 Ubiquitin-protein Ligase Itchy Homolog, Itch, Atrophin-1-interacting Protein 4, AIP4, NFE2-associated Polypeptide 1, NAPP1, DJ468O1.1) APC
Product Gene Name
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
1B8
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human ITCH.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
99kDa
3D Structure
ModBase 3D Structure for A9Z1X3
Immunogen
Partial recombinant corresponding to aa92-191 from human ITCH (NP_113671) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
DVLLGTAALDIYETLKSNNMKLEEVVVTLQLGGDKEPTETIGDLSICLDGLQLESEVVTNGETTCSESASQNDDGSRSKDETRVSTNGSDDPEDAGAGE
Conjugate
APC

Product Categories/Family for anti-ITCH antibody

Applications Tested/Suitable for anti-ITCH antibody
ELISA (EIA), Immunofluorescence (IF)
Application Notes for anti-ITCH antibody
IF: 10ug/ml
Applications are based on unconjugated antibody.

Immunofluorescence (IF) of anti-ITCH antibody

Immunofluorescence of monoclonal antibody to ITCH on HeLa cell. [antibody concentration 10ug/ml].
Mouse Anti-ITCH Monoclonal Antibody – Cat# MBS6137213

Testing Data of anti-ITCH antibody

Detection limit for recombinant GST tagged ITCH is 1ng/ml as a capture antibody.
Mouse Anti-ITCH Monoclonal Antibody, Testing Data – Cat# MBS6137213

Other Notes

Small volumes of anti-ITCH antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ITCH. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
E3 ubiquitin-protein ligase Itchy homolog isoform 2
NCBI Official Synonym Full Names
itchy E3 ubiquitin protein ligase
NCBI Official Symbol
ITCH
NCBI Official Synonym Symbols
AIF4; AIP4; ADMFD; NAPP1
NCBI Protein Information
E3 ubiquitin-protein ligase Itchy homolog

NCBI Summary for ITCH

This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein plays a role in multiple cellular processes including erythroid and lymphoid cell differentiation and the regulation of immune responses. Mutations in this gene are a cause of syndromic multisystem autoimmune disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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