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MPP1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6137666

Palmitoylated membrane protein 1 is the prototype of a family of membrane-associated proteins termed MAGUKs (membrane-associated guanylate kinase homologs). MAGUKs interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and… More details

$1155 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MPP1, Monoclonal Antibody
Full Product Name
MPP1 (55kD Erythrocyte Membrane Protein, p55, Membrane Protein, Palmitoylated 1, DXS552E, EMP55) APC
Product Gene Name
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
1C3-1D11
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human MPP1.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
49,854 Da
Immunogen
Full length recombinant corresponding to aa1-466 from human MPP1 (AAH02392) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MTLKASEGESGGSMHTALSDLYLEHLLQKRSRPEAVSHPLNTVTEDMYTNGSPAPGSPAQVKGQEVRKVRLIQFEKVTEEPMGITLKLNEKQSCTVARILHGGMIHRQGSLHVGDEILEINGTNVTNHSVDQLQKAMKETKGMISLKVIPNQQSRLPALQMFMRAQFDYDPKKDNLIPCKEAGLKFATGDIIQIINKDDSNWWQGRVEGSSKESAGLIPSPELQEWRVASMAQSAPSEAPSCSPFGKKKKYKDKYLAKHSSIFDQLDVVSYEEVVRLPAFKRKTLVLIGASGVGRSHIKNALLSQNPEKFVYPVPYTTRPPRKSEEDGKEYHFISTEEMTRNISANEFLEFGSYQGNMFGTKFETVHQIHKQNKIAILDIEPQTLKIVRTAELSPFIVFIAPTDQGTQTEALQQLQKDSEAIRSQYAHYFDLSLVNNGVDETLKKLQEAFDQACSSPQWVPVSWVY
Conjugate
APC

Product Categories/Family for anti-MPP1 antibody

Applications Tested/Suitable for anti-MPP1 antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-MPP1 antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-MPP1 antibody

Western Blot detection against Immunogen (77kD).
Mouse Anti-MPP1 Monoclonal Antibody – Cat# MBS6137666

Western Blot (WB) of anti-MPP1 antibody

MPP1 monoclonal antibody Western Blot analysis of MPP1 expression in K-562.
Mouse Anti-MPP1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6137666

Western Blot (WB) of anti-MPP1 antibody

Western Blot analysis of MPP1 expression in transfected 293T cell line by MPP1 monoclonal antibody. Lane 1: MPP1 transfected lysate (52.3kD). Lane 2: Non-transfected lysate.
Mouse Anti-MPP1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6137666

Testing Data of anti-MPP1 antibody

Detection limit for recombinant GST tagged MPP1 is ~3ng/ml as a capture antibody.
Mouse Anti-MPP1 Monoclonal Antibody, Testing Data – Cat# MBS6137666

Western Blot (WB) of anti-MPP1 antibody

Western blot analysis of MPP1 over-expressed 293 cell line, cotransfected with MPP1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with MPP1 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.
Mouse Anti-MPP1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6137666

Other Notes

Small volumes of anti-MPP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MPP1 purchase

MBS6137666 is an antibody targeting MPP1. Also known as MPP1 (55kD Erythrocyte Membrane Protein, p55, Membrane Protein, Palmitoylated 1, DXS552E, EMP55) APC. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for MPP1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Homo sapiens membrane protein, palmitoylated 1, 55kDa, mRNA
NCBI Official Synonym Full Names
membrane palmitoylated protein 1
NCBI Official Symbol
MPP1
NCBI Official Synonym Symbols
MRG1; PEMP; AAG12; EMP55; DXS552E
NCBI Protein Information
55 kDa erythrocyte membrane protein

NCBI Summary for MPP1

This gene encodes the prototype of the membrane-associated guanylate kinase (MAGUK) family proteins. MAGUKs interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intercellular junctions. The encoded protein is an extensively palmitoylated membrane phosphoprotein containing a PDZ domain, a Src homology 3 (SH3) motif, and a guanylate kinase domain. This gene product interacts with various cytoskeletal proteins and cell junctional proteins in different tissue and cell types, and may be involved in the regulation of cell shape, hair cell development, neural patterning of the retina, and apico-basal polarity and tumor suppression pathways in non-erythroid cells. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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