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NCK1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6137802

NCK adaptor protein 1, also known as NCK, belongs to the adaptor family of proteins. The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and… More details

$1155 / 0.1 mL +1 FREE 8GB USB
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
NCK1, Monoclonal Antibody
Full Product Name
NCK1 (Cytoplasmic Protein Nck1, MGC12668, Nck1, Nck-1, Nck Adaptor Protein 1, SH2/SH3 Adaptor Protein NCK-alpha, SH2/SH3 Adaptor Protein NCK-alpha, NCK) APC
Product Gene Name
Clonality
Monoclonal
Isotype
IgG
Clone Number
1A1
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human NCK1.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
35,511 Da
Immunogen
Partial recombinant corresponding to aa185-294 from human NCK1 (AAH06403) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
NLNTGQVLHVVQALYPFSSSNDEELNFEKGDVMDVIEKPENDPEWWKCRKINGMVGLVPKNYVTVMQNNPLTSGLEPSPPQCDYIRPSLTGKFAGNPWYYGKVTRHQAEM
Conjugate
APC

Product Categories/Family for anti-NCK1 antibody

Applications Tested/Suitable for anti-NCK1 antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-NCK1 antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-NCK1 antibody

Western Blot detection against Immunogen (37.73KD).
Mouse Anti-NCK1 Monoclonal Antibody – Cat# MBS6137802

Western Blot (WB) of anti-NCK1 antibody

NCK1 monoclonal antibody Western Blot analysis of NCK1 expression in HeLa NE.
Mouse Anti-NCK1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6137802

Western Blot (WB) of anti-NCK1 antibody

Western Blot analysis of NCK1 expression in transfected 293T cell line by NCK1 monoclonal antibody. Lane 1: NCK1 transfected lysate (42.9KD). Lane 2: Non-transfected lysate.
Mouse Anti-NCK1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6137802

Testing Data of anti-NCK1 antibody

Detection limit for recombinant GST tagged NCK1 is ~0.1ng/ml as a capture antibody.
Mouse Anti-NCK1 Monoclonal Antibody, Testing Data – Cat# MBS6137802

Other Notes

Small volumes of anti-NCK1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NCK1 purchase

MBS6137802 is an antibody targeting NCK1. Also known as NCK1 (Cytoplasmic Protein Nck1, MGC12668, Nck1, Nck-1, Nck Adaptor Protein 1, SH2/SH3 Adaptor Protein NCK-alpha, SH2/SH3 Adaptor Protein NCK-alpha, NCK) APC. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for NCK1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Homo sapiens NCK adaptor protein 1, mRNA
NCBI Official Synonym Full Names
NCK adaptor protein 1
NCBI Official Symbol
NCK1
NCBI Official Synonym Symbols
NCK; nck-1; NCKalpha
Protein Family
NCBI Protein Information
cytoplasmic protein NCK1

NCBI Summary for NCK1

The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq, Jun 2010]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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