• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

NISCH Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6137885

Acts either as the functional imidazoline-1 receptor (I1R) candidate or as a membrane-associated mediator of the I1R signaling. Binds numerous imidazoline ligands that induces initiation of cell-signaling cascades triggering to cell survival, growth and… More details

$1155 / 0.1 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NISCH, Monoclonal Antibody
Full Product Name
NISCH (Nischarin, Imidazoline Receptor 1, I-1, IR1, Imidazoline Receptor Antisera-selected Protein, hIRAS, Imidazoline-1 Receptor, I1R, Imidazoline-1 Receptor Candidate Protein, I-1 Receptor Candidate Protein, I1R Candidate Protein, IRAS, KIAA0975) APC
Product Gene Name
anti-NISCH antibody
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
2C8
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human NISCH.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Immunogen
Partial recombinant corresponding to aa1246-1346 from NISCH (AAH56900) with GST tag. MW of the GST tag alone is 26kD.
Conjugate
APC

Product Categories/Family for anti-NISCH antibody

Antibodies; Receptors
Applications Tested/Suitable for anti-NISCH antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-NISCH antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-NISCH antibody

Western Blot detection against Immunogen (37.11kD).
Mouse Anti-NISCH Monoclonal Antibody – Cat# MBS6137885

Testing Data of anti-NISCH antibody

Detection limit for recombinant GST tagged NISCH is ~0.3ng/ml as a capture antibody.
Mouse Anti-NISCH Monoclonal Antibody, Testing Data – Cat# MBS6137885

Other Notes

Small volumes of anti-NISCH antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NISCH purchase

MBS6137885 is an antibody targeting NISCH. Also known as NISCH (Nischarin, Imidazoline Receptor 1, I-1, IR1, Imidazoline Receptor Antisera-selected Protein, hIRAS, Imidazoline-1 Receptor, I1R, Imidazoline-1 Receptor Candidate Protein, I-1 Receptor Candidate Protein, I1R Candidate Protein, IRAS, KIAA0975) APC. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for NISCH. It may not necessarily be applicable to this product.

NCBI GI #
34784911
NCBI GeneID
11188
NCBI Accession #
BC056900
NCBI Related Accession #
AAH56900
UniProt Related Accession #
Q9Y2I1
NCBI Official Full Name
Homo sapiens nischarin, mRNA
NCBI Official Synonym Full Names
nischarin
NCBI Official Symbol
NISCH
NCBI Official Synonym Symbols
I-1; IR1; IRAS; hIRAS
Protein Family
Nischarin
NCBI Protein Information
nischarin

NCBI Summary for NISCH

This gene encodes a nonadrenergic imidazoline-1 receptor protein that localizes to the cytosol and anchors to the inner layer of the plasma membrane. The orthologous mouse protein has been shown to influence cytoskeletal organization and cell migration by binding to alpha-5-beta-1 integrin. In humans, this protein has been shown to bind to the adapter insulin receptor substrate 4 (IRS4) to mediate translocation of alpha-5 integrin from the cell membrane to endosomes. Expression of this protein was reduced in human breast cancers while its overexpression reduced tumor growth and metastasis; possibly by limiting the expression of alpha-5 integrin. In human cardiac tissue, this gene was found to affect cell growth and death while in neural tissue it affected neuronal growth and differentiation. Alternative splicing results in multiple transcript variants encoding differerent isoforms. Some isoforms lack the expected C-terminal domains of a functional imidazoline receptor. [provided by RefSeq, Jan 2013]
Show more

Research Articles on NISCH

1. These experiments demonstrate an important role of Nischarin in regulating cell attachment, which adds to our understanding of the early events of the metastatic process in breast cancer.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000