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Nkx 2.5 (NKX2.5) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6137893

NKX2.5 is a member of the NKX homeobox transcription factor family. NKX2.5 plays an essential role in heart development and is among the earliest factors expressed in cardiac lineage of developing embryos. Its targeted disruption in mice causes abnormal heart… More details

$1155 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Nkx 2.5 (NKX2.5), Monoclonal Antibody
Full Product Name
Nkx 2.5 (Homeobox Protein Nkx-2.5, Cardiac-specific, CSX, NK-2 Homolog E, NKX2.5, NKX2E, FLJ52202, FLJ97166, FLJ97195, FLJ97197, FLJ99536) APC
Product Gene Name
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
1E4-G5
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human NKX2-5.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
11,681 Da
Immunogen
Full length recombinant corresponding to aa1-324 from human NKX2-5 (AAH25711) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MFPSPALTPTPFSVKDILNLEQQQRSLAAAGELSARLEATLAPSSCMLAAFKPEAYAGPEAAAPGLPELRAELGRAPSPAKCASAFPAAPAFYPRAYSDPDPAKDPRAEKKELCALQKAVELEKTEADNAERPRARRRRKPRVLFSQAQVYELERRFKQQRYLSAPERDQLASVLKLTSTQVKIWFQNRRYKCKRQRQDQTLELVGLPPPPPPPARRIAVPVLVRDGKPCLGDSAPYAPAYGVGLNPYGYNAYPAYPGYGGAACSPGYSCTAAYPAGPSPAQPATAAANNNFVNFGVGDLNAVQSPGIPQSNSGVSTLHGIRAW
Conjugate
APC
Applications Tested/Suitable for anti-NKX2.5 antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-NKX2.5 antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-NKX2.5 antibody

Western Blot detection against Immunogen (61.38kD).
Mouse Anti-NKX2.5 Monoclonal Antibody – Cat# MBS6137893

Western Blot (WB) of anti-NKX2.5 antibody

NKX2-5 monoclonal antibody. Western Blot analysis of NKX2-5 expression in human thyroid (diffuse hyperplasia).
Mouse Anti-NKX2.5 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6137893

Western Blot (WB) of anti-NKX2.5 antibody

NKX2-5 monoclonal antibody, Western Blot analysis of NKX2-5 expression in HeLa.
Mouse Anti-NKX2.5 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6137893

Western Blot (WB) of anti-NKX2.5 antibody

Western Blot analysis of NKX2-5 expression in transfected 293T cell line by NKX2-5 monoclonal antibody. Lane 1: NKX2-5 transfected lysate (34.9kD). Lane 2: Non-transfected lysate.
Mouse Anti-NKX2.5 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6137893

Testing Data of anti-NKX2.5 antibody

Detection limit for recombinant GST tagged NKX2-5 is ~0.03ng/ml as a capture antibody.
Mouse Anti-NKX2.5 Monoclonal Antibody, Testing Data – Cat# MBS6137893

Product References and Citations for anti-NKX2.5 antibody

1. Complex SUMO-1 Regulation of Cardiac Transcription Factor Nkx2-5. Costa MW, Lee S, Furtado MB, Xin L, Sparrow DB, Martinez CG, Dunwoodie SL, Kurtenbach E, Mohun T, Rosenthal N, Harvey RP.PLoS One. 2011;6(9):e24812. Epub 2011 Sep 12. 2. RNA toxicity in myotonic muscular dystrophy induces NKX2-5 expression. Yadava RS, Frenzel-McCardell CD, Yu Q, Srinivasan V, Tucker AL, Puymirat J, Thornton CA, Prall OW, Harvey RP, Mahadevan MS.Nat Genet. 2008 Jan;40(1):61-8. Epub 2007 Dec 16.

Other Notes

Small volumes of anti-NKX2.5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NKX2.5 purchase

MBS6137893 is an antibody targeting NKX2.5. Also known as Nkx 2.5 (Homeobox Protein Nkx-2.5, Cardiac-specific, CSX, NK-2 Homolog E, NKX2.5, NKX2E, FLJ52202, FLJ97166, FLJ97195, FLJ97197, FLJ99536) APC. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for NKX2.5. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Homo sapiens NK2 transcription factor related, locus 5 (Drosophila), mRNA
NCBI Official Synonym Full Names
NK2 homeobox 5
NCBI Official Symbol
NKX2-5
NCBI Official Synonym Symbols
CSX; CSX1; VSD3; CHNG5; HLHS2; NKX2E; NKX2.5; NKX4-1
Protein Family
NCBI Protein Information
homeobox protein Nkx-2.5

NCBI Summary for NKX2.5

This gene encodes a homeobox-containing transcription factor. This transcription factor functions in heart formation and development. Mutations in this gene cause atrial septal defect with atrioventricular conduction defect, and also tetralogy of Fallot, which are both heart malformation diseases. Mutations in this gene can also cause congenital hypothyroidism non-goitrous type 5, a non-autoimmune condition. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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