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NME2 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6137901

NM2 is a heterodimeric protein functioning as a nucleoside diphosphate (NDP) kinase. NME1 and NME2 comprise the 152 amino acid A and B polypeptide chains of the NM23 enzyme, respectively. NME2 is identical to the beta subunit of human erythrocyte NDP kinase.… More details

$1155 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NME2, Monoclonal Antibody
Full Product Name
NME2 (Nucleoside Diphosphate Kinase B, NDK B, NDP Kinase B, nm23-H2, C-myc Purine-binding Transcription Factor PUF, NM23B) APC
Product Gene Name
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
4B7-3F12
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human NME2.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Immunogen
Full length recombinant corresponding to aa1-153 from human NME2 (AAH02476) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MANLERTFIAIKPDGVQRGLVGEIIKRFEQKGFRLVAMKFLRASEEHLKQHYIDLKDRPFFPGLVKYMNSGPVVAMVWEGLNVVKTGRVMLGETNPADSKPGTIRGDFCIQVGRNIIHGSDSVKSAEKEISLWFKPEELVDYKSCAHDWVYE
Conjugate
APC

Product Categories/Family for anti-NME2 antibody

Applications Tested/Suitable for anti-NME2 antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-NME2 antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-NME2 antibody

Western Blot detection against Immunogen (42.46kD).
Mouse Anti-NME2 Monoclonal Antibody – Cat# MBS6137901

Western Blot (WB) of anti-NME2 antibody

NME2 monoclonal antibody Western Blot analysis of NME2 expression in Jurkat.
Mouse Anti-NME2 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6137901

Western Blot (WB) of anti-NME2 antibody

Western Blot analysis of NME2 expression in transfected 293T cell line by NME2 monoclonal antibody. Lane 1: NME2 transfected lysate (17.3kD). Lane 2: Non-transfected lysate.
Mouse Anti-NME2 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6137901

Testing Data of anti-NME2 antibody

Detection limit for recombinant GST tagged NME2 is ~0.03ng/ml as a capture antibody.
Mouse Anti-NME2 Monoclonal Antibody, Testing Data – Cat# MBS6137901

Western Blot (WB) of anti-NME2 antibody

Western blot analysis of NME2 over-expressed 293 cell line, cotransfected with NME2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with NME2 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.
Mouse Anti-NME2 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6137901

Other Notes

Small volumes of anti-NME2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NME2 purchase

MBS6137901 is an antibody targeting NME2. Also known as NME2 (Nucleoside Diphosphate Kinase B, NDK B, NDP Kinase B, nm23-H2, C-myc Purine-binding Transcription Factor PUF, NM23B) APC. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for NME2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Related Accession #
NCBI Official Full Name
Homo sapiens non-metastatic cells 2, protein (NM23B) expressed in, mRNA
NCBI Official Synonym Full Names
NME/NM23 nucleoside diphosphate kinase 2
NCBI Official Symbol
NME2
NCBI Official Synonym Symbols
PUF; NDKB; NDPKB; NM23B; NDPK-B; NM23-H2
NCBI Protein Information
nucleoside diphosphate kinase B

NCBI Summary for NME2

Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by NME1) and 'B' (encoded by this gene) isoforms. Multiple alternatively spliced transcript variants have been found for this gene. Read-through transcription from the neighboring upstream gene (NME1) generates naturally-occurring transcripts (NME1-NME2) that encode a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq, Nov 2010]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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