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Nf-Y Beta (NFYB) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS613799

MBS613799 is an antibody targeting NFYB. Also known as NF-Y beta (NF-YB, Nuclear Factor Yb). Reactive in Human, Mouse. Host: Rabbit. More details

$1140 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NF-Y beta (NFYB), Polyclonal Antibody
Full Product Name
NF-Y beta (NF-YB, Nuclear Factor Yb)
Product Gene Name
Product Synonym Names
Anti -NF-Y beta (NF-YB, Nuclear Factor Yb)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. No stabilizing proteins added.
Purity/Purification
Highly Purified
Purified by delipidation, salt fractionation and ion exchange chromatography.
Specificity
Recognizes the human and mouse NF-Y (B subunit) protein at 25kD.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
22,831 Da
Chromosome Location
Chromosome: 12; NC_000012.11 (104510858..104532040, complement). Location: 12q22-q23
OMIM
189904
3D Structure
ModBase 3D Structure for P25208
Immunogen
A synthetic peptide corresponding to a region near the N-terminus of the human NF-Y (B subunit) protein (KLH).
Applications Tested/Suitable for anti-NFYB antibody
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-NFYB antibody
Suitable for Immunoprecipitation, Immunoblot, ELISA and Supershift assays.
Dilution: ELISA: 1:4000
Immunoblot: 1:500
Super shift: 2-4ul/assay.

Other Notes

Small volumes of anti-NFYB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NFYB. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
nuclear transcription factor Y subunit beta
NCBI Official Synonym Full Names
nuclear transcription factor Y, beta
NCBI Official Symbol
NFYB
NCBI Official Synonym Symbols
HAP3; CBF-A; CBF-B; NF-YB
UniProt Protein Name
Nuclear transcription factor Y subunit beta
UniProt Synonym Protein Names
CAAT box DNA-binding protein subunit B; Nuclear transcription factor Y subunit B
UniProt Gene Name
NFYB
UniProt Synonym Gene Names
HAP3
UniProt Entry Name
NFYB_HUMAN
NCBI Protein Information
nuclear transcription factor Y subunit beta; Transcription factor NF-Y, B subunit; CAAT box DNA-binding protein subunit B; nuclear transcription factor Y subunit B; CCAAT-binding transcription factor subunit A

NCBI Summary for NFYB

The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants. [provided by RefSeq]
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UniProt Comments for NFYB

NFYB: Stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes. Belongs to the NFYB/HAP3 subunit family.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 12q22-q23

Cellular Component: nucleoplasm; CCAAT-binding factor complex; nucleus

Molecular Function: protein binding; DNA binding; protein heterodimerization activity; sequence-specific DNA binding; protein complex binding; transcription factor activity

Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; cellular lipid metabolic process
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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