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PGM3 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6138205

MBS6138205 is an antibody targeting PGM3. Also known as PGM3 (Phosphoacetylglucosamine Mutase, PAGM, Acetylglucosamine Phosphomutase, N-acetylglucosamine-phosphate Mutasem Phosphoglucomutase-3, AGM1, DKFZp434B187) APC. Reactive in Human. Host: Mouse. More details

$1155 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PGM3, Monoclonal Antibody
Full Product Name
PGM3 (Phosphoacetylglucosamine Mutase, PAGM, Acetylglucosamine Phosphomutase, N-acetylglucosamine-phosphate Mutasem Phosphoglucomutase-3, AGM1, DKFZp434B187) APC
Product Gene Name
anti-PGM3 antibody
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
1E2-1B12
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human PGM3.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
62,941 Da
Immunogen
Full length recombinant corresponding to aa1-543 from human PGM3 (AAH01258) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MDLGAITKYSALHAKPNGLILQYGTAGFRTKAEHLDHVMFRMGLLAVLRSKQTKSTIGVMVTASHNPEEDNGVKLVDPLGEMLAPSWEEHATCLANAEEQDMQRVLIDISEKEAVNLQQDAFVVIGRDTRPSSEKLSQSVIDGVTVLGGQFHDYGLLTTPQLHYMVYCRNTGGRYGKATIEGYYQKLSKAFVELTKQASCSGDEYRSLKVDCANGIGALKLREMEHYFSQGLSVQLFNDGSKGKLNHLCGADFVKSHQKPPQGMEIKSNERCCSFDGDADRIVYYYHDADGHFHLIDGDKIATLISSFLKELLVEIGESLNIGVVQTAYANGSSTRYLEEVMKVPVYCTKTGVKHLHHKAQEFDIGVYFEANGHGTALFSTAVEMKIKQSAEQLEDKKRKAAKMLENIIDLFNQAAGDAISDMLVIEAILALKGLTVQQWDALYTDLPNRQLKVQVADRRVISTTNAERQAVTPPGLQEAINDLVKKYKLSRAFVRPSGTEDVVRVYAEADSQESADHLAHEVSLAVFQLAGGIGERPQPGF
Conjugate
APC

Product Categories/Family for anti-PGM3 antibody

Antibodies; Enzymes
Applications Tested/Suitable for anti-PGM3 antibody
ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Immunoprecipitation (IP), Western Blot (WB)
Application Notes for anti-PGM3 antibody
IHC-P: 2ug/ml
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-PGM3 antibody

Western Blot detection against Immunogen (85.36kD).
Mouse Anti-PGM3 Monoclonal Antibody – Cat# MBS6138205

Western Blot (WB) of anti-PGM3 antibody

PGM3 monoclonal antibody Western Blot analysis of PGM3 expression in HeLa.
Mouse Anti-PGM3 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6138205

Western Blot (WB) of anti-PGM3 antibody

Western Blot analysis of PGM3 expression in transfected 293T cell line by PGM3 monoclonal antibody. Lane 1: PGM3 transfected lysate (59.9kD). Lane 2: Non-transfected lysate.
Mouse Anti-PGM3 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6138205

Immunohistochemistry (IHC) of anti-PGM3 antibody

Immunoperoxidase of monoclonal antibody to PGM3 on formalin-fixed paraffin-embedded human colon tissue. [antibody concentration 2ug/ml]
Mouse Anti-PGM3 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6138205

Immunoprecipitation (IP) of anti-PGM3 antibody

Immunoprecipitation of PGM3 transfected lysate using PGM3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PGM3 rabbit polyclonal antibody.
Mouse Anti-PGM3 Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS6138205

Testing Data of anti-PGM3 antibody

Detection limit for recombinant GST tagged PGM3 is ~3ng/ml as a capture antibody.
Mouse Anti-PGM3 Monoclonal Antibody, Testing Data – Cat# MBS6138205

Western Blot (WB) of anti-PGM3 antibody

Western blot analysis of PGM3 over-expressed 293 cell line, cotransfected with PGM3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PGM3 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.
Mouse Anti-PGM3 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6138205

Other Notes

Small volumes of anti-PGM3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PGM3. It may not necessarily be applicable to this product.

NCBI GI #
12654830
NCBI GeneID
5238
NCBI Accession #
BC001258
NCBI Related Accession #
AAH01258
UniProt Secondary Accession #
Q5JWR4; Q96J46; Q9H8G5; Q9NS94; Q9NTT6; Q9UFV5; B2RB65; B4DX94; D6RF12; E1P547; E9PF86
UniProt Related Accession #
O95394
NCBI Official Full Name
Homo sapiens phosphoglucomutase 3, mRNA
NCBI Official Synonym Full Names
phosphoglucomutase 3
NCBI Official Symbol
PGM3
NCBI Official Synonym Symbols
AGM1; PAGM; IMD23; PGM 3
NCBI Protein Information
phosphoacetylglucosamine mutase; N-acetylglucosamine-phosphate mutase 1; acetylglucosamine phosphomutase

NCBI Summary for PGM3

This gene encodes a member of the phosphohexose mutase family. The encoded protein mediates both glycogen formation and utilization by catalyzing the interconversion of glucose-1-phosphate and glucose-6-phosphate. A non-synonymous single nucleotide polymorphism in this gene may play a role in resistance to diabetic nephropathy and neuropathy. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2010]
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Research Articles on PGM3

1. define PGM3-CDG as a treatable immunodeficiency, document the power of whole-exome sequencing in gene discoveries for rare disorders, and illustrate the utility of genomic analyses in studying combined and variable phenotypes

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-PGM3 antibody

 Products by Pathway  Pathway Diagram
 Amino Sugar And Nucleotide Sugar Metabolism Pathway antibodies  Amino Sugar And Nucleotide Sugar Metabolism Pathway Diagram
 Amino Sugar And Nucleotide Sugar Metabolism Pathway antibodies  Amino Sugar And Nucleotide Sugar Metabolism Pathway Diagram
 Asparagine N-linked Glycosylation Pathway antibodies  Asparagine N-linked Glycosylation Pathway Diagram
 Biosynthesis Of The N-glycan Precursor (dolichol Lipid-linked Oligosaccharide, LLO) And Transfer To A Nascent Protein Pathway antibodies  Biosynthesis Of The N-glycan Precursor (dolichol Lipid-linked Oligosaccharide, LLO) And Transfer To A Nascent Protein Pathway Diagram
 Defective ALG1 Causes ALG1-CDG (CDG-1k) Pathway antibodies  Defective ALG1 Causes ALG1-CDG (CDG-1k) Pathway Diagram
 Defective ALG11 Causes ALG11-CDG (CDG-1p) Pathway antibodies  Defective ALG11 Causes ALG11-CDG (CDG-1p) Pathway Diagram
 Defective ALG12 Causes ALG12-CDG (CDG-1g) Pathway antibodies  Defective ALG12 Causes ALG12-CDG (CDG-1g) Pathway Diagram
 Defective ALG14 Causes Congenital Myasthenic Syndrome (ALG14-CMS) Pathway antibodies  Defective ALG14 Causes Congenital Myasthenic Syndrome (ALG14-CMS) Pathway Diagram
 Defective ALG2 Causes ALG2-CDG (CDG-1i) Pathway antibodies  Defective ALG2 Causes ALG2-CDG (CDG-1i) Pathway Diagram
 Defective ALG3 Causes ALG3-CDG (CDG-1d) Pathway antibodies  Defective ALG3 Causes ALG3-CDG (CDG-1d) Pathway Diagram

Diseases associated with anti-PGM3 antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >3 publications with PGM3 and Nervous System Diseases
 Mental Disorders Antibodies  >2 publications with PGM3 and Mental Disorders
 Memory Disorders Antibodies  >1 publications with PGM3 and Memory Disorders
 Fibrosis Antibodies  >1 publications with PGM3 and Fibrosis
 Abnormalities, Multiple Antibodies  >1 publications with PGM3 and Abnormalities, Multiple
 Fetal Death Antibodies  >1 publications with PGM3 and Fetal Death
 Brain Diseases Antibodies  >1 publications with PGM3 and Brain Diseases
 Cognition Disorders Antibodies  >1 publications with PGM3 and Cognition Disorders

Organs/Tissues associated with anti-PGM3 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >45 publications with PGM3 and Blood
 Placenta Antibodies  >11 publications with PGM3 and Placenta
 Embryonic Tissue Antibodies  >5 publications with PGM3 and Embryonic Tissue
 Muscle Antibodies  >4 publications with PGM3 and Muscle
 Kidney Antibodies  >3 publications with PGM3 and Kidney
 Skin Antibodies  >3 publications with PGM3 and Skin
 Bone Antibodies  >3 publications with PGM3 and Bone
 Brain Antibodies  >3 publications with PGM3 and Brain
 Bone Marrow Antibodies  >2 publications with PGM3 and Bone Marrow
 Liver Antibodies  >2 publications with PGM3 and Liver
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