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Protein Synthesis Initiation Factor 4g Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS613829

Eukaryotic initiation factor 4E (eIF4E) binds to the mRNA cap structure, thereby mediating the initiation of translation (1,2). eIF4E interacts with eIF4G, which serves as a scaffold protein for the assembly of eIF4E and eIF4A to form the eIF4F complex (2).… More details

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Product Overview

Online Datasheet in PDF
Product Name
Protein Synthesis Initiation Factor 4G, Polyclonal Antibody
Full Product Name
Protein Synthesis Initiation Factor 4G, phosphorylated (Ser1108) (eIF 4G, Eukaryotic Translation Initiation Factor)
Product Synonym Names
Anti -Protein Synthesis Initiation Factor 4G, phosphorylated (Ser1108) (eIF 4G, Eukaryotic Translation Initiation Factor)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Bovine, Hamster, Human, Monkey, Mouse, Rat
Form/Format
Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Recognizes human eIF4GI only when phosphorylated at Ser1108. Does not crossreact with non-phosphorylated eIF4GI or p97. Species Crossreactivity: bovine, hamster, monkey, mouse and rat.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Ser1108 of human eIF4GI (KLH).
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF)
Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunocytochemistry and Immunofluorescence.
Dilution: Western Blot: 1:1000
Immunoprecipitation: 1:100
Immunofluorescence (IF-IC): 1:100

Western Blot (WB) of anti-Protein Synthesis Initiation Factor 4G antibody

Western blot analysis of extracts from PC12 cells, untreated (lane 1), NGF-treated (10 ng/ml) (lane 2), anisomycin-treated (25uM) (lane 3), U0126-treated (10uM) M2865-50 (lane 4), Rapamycin-treated (100nM ) (lane 5) or LY294002-treated (25uM) (lane 6), using MBS613829.
Rabbit Anti-Protein Synthesis Initiation Factor 4g Polyclonal Antibody – Cat# MBS613829

Western Blot (WB) of anti-Protein Synthesis Initiation Factor 4G antibody

Western blot analysis of extracts from 293 cells expressing GST-eIF4GI Ser1192Ala or GST-eIF4GI Ser1108Ala mutant protein, using MBS613829.
Rabbit Anti-Protein Synthesis Initiation Factor 4g Polyclonal Antibody, Western Blot (WB) – Cat# MBS613829

Immunohistochemistry (IHC) of anti-Protein Synthesis Initiation Factor 4G antibody

Immunohistochemical staining of paraffin-embedded human breast carcinoma, showing cytoplasmic localization of phosphorylated eIF4G, using MBS613829.
Rabbit Anti-Protein Synthesis Initiation Factor 4g Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS613829

Immunofluorescence (IF) of anti-Protein Synthesis Initiation Factor 4G antibody

Immunofluorescent detection of HeLa cells, untreated (left) or thapsigargin-treated (300nM) (right), using MBS613829.
Rabbit Anti-Protein Synthesis Initiation Factor 4g Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS613829

Other Notes

Small volumes of anti-Protein Synthesis Initiation Factor 4G antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Protein Synthesis Initiation Factor 4G purchase

MBS613829 is an antibody targeting Protein Synthesis Initiation Factor 4G. Also known as Protein Synthesis Initiation Factor 4G, phosphorylated (Ser1108) (eIF 4G, Eukaryotic Translation Initiation Factor). Reactive in Bovine, Hamster, Human, Monkey, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Protein Synthesis Initiation Factor 4G. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Official Full Name
protein synthesis initiation factor 4G

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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