• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

PTPN22 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6138523

MBS6138523 is an antibody targeting PTPN22. Also known as PTPN22 (Tyrosine-protein Phosphatase Non-receptor Type 22, Hematopoietic Cell Protein-tyrosine Phosphatase 70Z-PEP, Lymphoid Phosphatase, LyP, PEST-domain Phosphatase, PEP, PTPN8) APC. Reactive in… More details

$1155 / 0.1 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PTPN22, Monoclonal Antibody
Full Product Name
PTPN22 (Tyrosine-protein Phosphatase Non-receptor Type 22, Hematopoietic Cell Protein-tyrosine Phosphatase 70Z-PEP, Lymphoid Phosphatase, LyP, PEST-domain Phosphatase, PEP, PTPN8) APC
Product Gene Name
Product Synonym Names
EC=3.1.3.48; LYP1; LYP2
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
4F6
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human PTPN22.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Immunogen
Full length recombinant corresponding to aa1-180 from human PTPN22 (AAH17785) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MDQREILQKFLDEAQSKKITKEEFANEFLKLKRQSTKYKADKTYPTTVAEKPKNIKKNRYKDILPYDYSRVELSLITSDEDSSYINANFIKGVYGPKAYIATQGPLSTTLLDFWRMIWEYSVLIIVMACMEYEMGKEAEKRKSDYIIRTLKVKFNSVSVILAHQTSLQNLFSQITPAHF*
Conjugate
APC

Product Categories/Family for anti-PTPN22 antibody

Applications Tested/Suitable for anti-PTPN22 antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-PTPN22 antibody
ELISA: 1ng/ml
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-PTPN22 antibody

Western Blot detection against Immunogen (45.8kD).
Mouse Anti-PTPN22 Monoclonal Antibody – Cat# MBS6138523

Western Blot (WB) of anti-PTPN22 antibody

Western Blot analysis of PTPN22 expression in transfected 293T cell line by PTPN22 monoclonal antibody. Lane 1: PTPN22 transfected lysate (85.1kD). Lane 2: Non-transfected lysate.
Mouse Anti-PTPN22 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6138523

Testing Data of anti-PTPN22 antibody

Detection limit for recombinant GST tagged PTPN22 is 1ng/ml as a capture antibody.
Mouse Anti-PTPN22 Monoclonal Antibody, Testing Data – Cat# MBS6138523

Western Blot (WB) of anti-PTPN22 antibody

Western blot analysis of PTPN22 over-expressed 293 cell line, cotransfected with PTPN22 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PTPN22 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.
Mouse Anti-PTPN22 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6138523

Other Notes

Small volumes of anti-PTPN22 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PTPN22. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Related Accession #
NCBI Official Full Name
Homo sapiens protein tyrosine phosphatase, non-receptor type 22 (lymphoid), mRNA
NCBI Official Synonym Full Names
protein tyrosine phosphatase non-receptor type 22
NCBI Official Symbol
PTPN22
NCBI Official Synonym Symbols
LYP; PEP; LYP1; LYP2; PTPN8; PTPN22.5; PTPN22.6
NCBI Protein Information
tyrosine-protein phosphatase non-receptor type 22

NCBI Summary for PTPN22

This gene encodes of member of the non-receptor class 4 subfamily of the protein-tyrosine phosphatase family. The encoded protein is a lymphoid-specific intracellular phosphatase that associates with the molecular adapter protein CBL and may be involved in regulating CBL function in the T-cell receptor signaling pathway. Mutations in this gene may be associated with a range of autoimmune disorders including Type 1 Diabetes, rheumatoid arthritis, systemic lupus erythematosus and Graves' disease. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Mar 2009]
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000