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SIM2 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6139059

Single-minded homolog 2 is a transcription factor that may be a master gene of CNS development in cooperation with Arnt. It may have pleiotropic effects in the tisues expressed during development. Efficient DNA binding requires dimerisation with another bHLH… More details

$1155 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
SIM2, Monoclonal Antibody
Full Product Name
SIM2 (Single-minded Homolog 2, Class E Basic Helix-loop-helix Protein 15, bHLHe15, BHLHE15, MGC119447) APC
Product Gene Name
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
3E6
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human SIM2.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
73kDa
OMIM
600892
3D Structure
ModBase 3D Structure for Q14190
Immunogen
Partial recombinant corresponding to aa426-526 from human SIM2 (NP_005060) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
ESSDLLYTPSYSLPFSYHYGHFPLDSHVFSSKKPMLPAKFGQPQGSPCEVARFFLSTLPASGECQWHYANPLVPSSSSPAKNPPEPPANTARHSLVPSYE*
Conjugate
APC
Applications Tested/Suitable for anti-SIM2 antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-SIM2 antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-SIM2 antibody

Western Blot detection against Immunogen (37.11kD).
Mouse Anti-SIM2 Monoclonal Antibody – Cat# MBS6139059

Testing Data of anti-SIM2 antibody

Detection limit for recombinant GST tagged SIM2 is ~0.03ng/ml as a capture antibody.
Mouse Anti-SIM2 Monoclonal Antibody, Testing Data – Cat# MBS6139059

Other Notes

Small volumes of anti-SIM2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SIM2 purchase

MBS6139059 is an antibody targeting SIM2. Also known as SIM2 (Single-minded Homolog 2, Class E Basic Helix-loop-helix Protein 15, bHLHe15, BHLHE15, MGC119447) APC. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for SIM2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
Single-minded homolog 2
NCBI Official Synonym Full Names
SIM bHLH transcription factor 2
NCBI Official Symbol
SIM2
NCBI Official Synonym Symbols
SIM; bHLHe15; HMC13F06; HMC29C01
UniProt Protein Name
Single-minded homolog 2
UniProt Synonym Protein Names
Class E basic helix-loop-helix protein 15; bHLHe15
Protein Family
UniProt Gene Name
SIM2
UniProt Synonym Gene Names
BHLHE15; bHLHe15
UniProt Entry Name
SIM2_HUMAN
NCBI Protein Information
single-minded homolog 2

NCBI Summary for SIM2

This gene represents a homolog of the Drosophila single-minded (sim) gene, which encodes a transcription factor that is a master regulator of neurogenesis. The encoded protein is ubiquitinated by RING-IBR-RING-type E3 ubiquitin ligases, including the parkin RBR E3 ubiquitin protein ligase. This gene maps within the so-called Down syndrome chromosomal region, and is thus thought to contribute to some specific Down syndrome phenotypes. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Sep 2014]
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UniProt Comments for SIM2

SIM2: Transcription factor that may be a master gene of CNS development in cooperation with Arnt. It may have pleiotropic effects in the tissues expressed during development. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; DNA-binding

Chromosomal Location of Human Ortholog: 21q22.13

Cellular Component: nucleoplasm

Molecular Function: signal transducer activity; DNA binding; protein heterodimerization activity; transcription factor activity

Biological Process: nervous system development; transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; embryonic pattern specification; cell differentiation; signal transduction; lung development
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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