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SMARCA2 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6139166

The protein encoded by this gene is a member of the SWI/SNF family of proteins and is highly similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by… More details

$1155 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
SMARCA2, Monoclonal Antibody
Full Product Name
SMARCA2 (Probable Global Transcription Activator SNF2L2, ATP-dependent Helicase SMARCA2, BRG1-associated Factor 190B, BAF190B, Protein Brahma Homolog, hBRM, SNF2-alpha, SWI/SNF-related Matrix-associated Actin-dependent Regulator of Chromatin Subfamily A M
Product Gene Name
anti-SMARCA2 antibody
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
2D12
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human SMARCA2.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
OMIM
X72889 mRNA
3D Structure
ModBase 3D Structure for P51531
Immunogen
Partial recombinant protein corresponding to aa659-735 from human SMARCA2 with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
KILLDPNSEEVSEKDAKQIIETAKQDVDDEYSMQYSARGSQSYYTVAHAISERVEKQSALLINGTLKHYQLQGLEWM*
Conjugate
APC

Product Categories/Family for anti-SMARCA2 antibody

Antibodies; Actin
Applications Tested/Suitable for anti-SMARCA2 antibody
ELISA (EIA), Immunofluorescence (IF), Western Blot (WB)
Application Notes for anti-SMARCA2 antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-SMARCA2 antibody

Western Blot detection against immunogen using 133550 (34.58kD).
Mouse Anti-SMARCA2 Monoclonal Antibody – Cat# MBS6139166

Testing Data of anti-SMARCA2 antibody

Detection limit is ~0.1ng/ml using 133550 as a capture antibody.
Mouse Anti-SMARCA2 Monoclonal Antibody, Testing Data – Cat# MBS6139166

Immunofluorescence (IF) of anti-SMARCA2 antibody

Immunofluorescence on HeLa cell using 133550 (10ug/ml).
Mouse Anti-SMARCA2 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS6139166

Other Notes

Small volumes of anti-SMARCA2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SMARCA2 purchase

MBS6139166 is an antibody targeting SMARCA2. Also known as SMARCA2 (Probable Global Transcription Activator SNF2L2, ATP-dependent Helicase SMARCA2, BRG1-associated Factor 190B, BAF190B, Protein Brahma Homolog, hBRM, SNF2-alpha, SWI/SNF-related Matrix-associated Actin-dependent Regulator of Chromatin Subfamily A M. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for SMARCA2. It may not necessarily be applicable to this product.

NCBI GI #
48255900
NCBI GeneID
6595
NCBI Accession #
NP_003061
UniProt Primary Accession #
P51531
UniProt Related Accession #
P51531
GenBank Nucleotide #
NM_003070
NCBI Official Full Name
probable global transcription activator SNF2L2 isoform a
NCBI Official Synonym Full Names
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2
NCBI Official Symbol
SMARCA2
NCBI Official Synonym Symbols
BRM; SNF2; SWI2; hBRM; NCBRS; Sth1p; BAF190; SNF2L2; SNF2LA; hSNF2a
UniProt Protein Name
Probable global transcription activator SNF2L2
UniProt Synonym Protein Names
ATP-dependent helicase SMARCA2; BRG1-associated factor 190B; BAF190B; Protein brahma homolog; hBRM; SNF2-alpha; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 2
Protein Family
Probable global transcription activator
UniProt Gene Name
SMARCA2
UniProt Synonym Gene Names
BAF190B; hBRM
NCBI Protein Information
probable global transcription activator SNF2L2

NCBI Summary for SMARCA2

The protein encoded by this gene is a member of the SWI/SNF family of proteins and is highly similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene, which contains a trinucleotide repeat (CAG) length polymorphism. [provided by RefSeq, Jan 2014]
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UniProt Comments for SMARCA2

SMARCA2: an enzyme with ATP-dependent DNA helicase activity. A transcriptional coactivator that cooperates with nuclear hormone receptors to potentiate transcriptional activation. Binds TOPBP1. Belongs to the SNF2/RAD54 helicase family. Two alternatively spliced human isoforms have been described.

Protein type: EC 3.6.1.-; EC 3.6.4.-; Helicase; Nuclear receptor co-regulator; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 9p24.3

Cellular Component: intermediate filament cytoskeleton; intracellular membrane-bound organelle; nBAF complex; npBAF complex; nuclear chromatin; nucleoplasm; nucleus; SWI/SNF complex

Molecular Function: ATP binding; chromatin binding; DNA-dependent ATPase activity; helicase activity; histone binding; protein binding; transcription coactivator activity; transcription regulatory region DNA binding

Biological Process: chromatin remodeling; negative regulation of cell growth; negative regulation of cell proliferation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; nervous system development; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-templated; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-templated; spermatid development; transcription from RNA polymerase II promoter

Disease: Nicolaides-baraitser Syndrome
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Research Articles on SMARCA2

1. targeting of BRM in combination with radiotherapy is supposed to improve the therapeutic outcome of lung cancer patients harboring BRG1 mutations.The present study shows that the moderate radioresponsiveness of NSCLC cells with BRG1 mutations can be increased upon BRM depletion that is associated with a prolonged Rad51-foci prevalence at DNA DSBs.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SMARCA2 antibody

 Reference Product  PubMed Publications
 SMARCA4 antibody  >197 publications with SMARCA2 and SMARCA4
 SMARCB1 antibody  >23 publications with SMARCA2 and SMARCB1
 ARID1A antibody  >18 publications with SMARCA2 and ARID1A
 SMARCE1 antibody  >11 publications with SMARCA2 and SMARCE1
 SMARCC1 antibody  >4 publications with SMARCA2 and SMARCC1
 SS18 antibody  >3 publications with SMARCA2 and SS18
 ACTL6A antibody  >3 publications with SMARCA2 and ACTL6A
 SMARCC2 antibody  >3 publications with SMARCA2 and SMARCC2
 SMARCD1 antibody  >1 publications with SMARCA2 and SMARCD1
 CEBPB antibody  >1 publications with SMARCA2 and CEBPB

Pathways associated with anti-SMARCA2 antibody

 Products by Pathway  Pathway Diagram
 C-MYB Transcription Factor Network Pathway antibodies  C-MYB Transcription Factor Network Pathway Diagram
 Chromatin Modifying Enzymes Pathway antibodies  Chromatin Modifying Enzymes Pathway Diagram
 Chromatin Organization Pathway antibodies  Chromatin Organization Pathway Diagram
 E2F Transcription Factor Network Pathway antibodies  E2F Transcription Factor Network Pathway Diagram
 RB In Cancer Pathway antibodies  RB In Cancer Pathway Diagram
 RMTs Methylate Histone Arginines Pathway antibodies  RMTs Methylate Histone Arginines Pathway Diagram
 Regulation Of Androgen Receptor Activity Pathway antibodies  Regulation Of Androgen Receptor Activity Pathway Diagram

Diseases associated with anti-SMARCA2 antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >15 publications with SMARCA2 and Nervous System Diseases
 Congenital Abnormalities Antibodies  >10 publications with SMARCA2 and Congenital Abnormalities
 Nicolaides Baraitser syndrome Antibodies  >9 publications with SMARCA2 and Nicolaides Baraitser syndrome
 Neurobehavioral Manifestations Antibodies  >8 publications with SMARCA2 and Neurobehavioral Manifestations
 Liver Neoplasms Antibodies  >8 publications with SMARCA2 and Liver Neoplasms
 Liver Diseases Antibodies  >7 publications with SMARCA2 and Liver Diseases
 Coffin-Siris syndrome Antibodies  >7 publications with SMARCA2 and Coffin-Siris syndrome
 Inflammation Antibodies  >6 publications with SMARCA2 and Inflammation
 Abnormalities, Multiple Antibodies  >6 publications with SMARCA2 and Abnormalities, Multiple
 Disease Models, Animal Antibodies  >6 publications with SMARCA2 and Disease Models, Animal

Organs/Tissues associated with anti-SMARCA2 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >20 publications with SMARCA2 and Brain
 Lung Antibodies  >18 publications with SMARCA2 and Lung
 Liver Antibodies  >16 publications with SMARCA2 and Liver
 Blood Antibodies  >13 publications with SMARCA2 and Blood
 Embryonic Tissue Antibodies  >11 publications with SMARCA2 and Embryonic Tissue
 Testis Antibodies  >9 publications with SMARCA2 and Testis
 Heart Antibodies  >8 publications with SMARCA2 and Heart
 Vascular Antibodies  >7 publications with SMARCA2 and Vascular
 Skin Antibodies  >6 publications with SMARCA2 and Skin
 Prostate Antibodies  >6 publications with SMARCA2 and Prostate
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