Eukaryotic Translation Initiation Factor 2b, Epsilon (EIF2B5) Antibody – Rabbit Polyclonal

Phosphorylation of the a subunit of eukaryotic initiation factor 2 is a well documented mechanism of down regulating protein synthesis under a variety of stress conditions. Eukaryotic initiation factor 2 binds GTP and Met-tRNAi and transfers the Met-tRNA to the 40S subunit to form the 43S preinitiation complex (1,2). For eIF2 to promote a new round of translation initiation, GDP must be exchanged for GTP, a reaction catalyzed by eIF2B (1,2). Kinases activated by viral infection (PKR), endoplasmic reticulum stress (PERK/PEK), amino acid deprivation (GCN2) and hemin deficiency (HRI) can phosphorylate the a subunit of eIF2 (3,4). This phosphorylation stabilizes the eIF2-GDP-eIF2B complex, inhibiting the turnover of eIF2B. Induction of PKR by IFN-g and TNF-a, or stress provoked by depletion of endoplasmic reticulum calcium levels, induces potent phosphorylation of eIF2a at Ser51 (5,6). eIF2B, a guanine nucleotide exchange factor, is composed of 5 subunits, the largest of which is eIF2B-e (7). Multiple in vivo phosphorylation sites have been identified on eIF2B-e (8). Casein Kinase II can phosphorylate eIF2B-e at Ser717/718 to allow for association with its substrate eIF2. Phosphorylation at Ser544 allows GSK-3 to phosphorylate the key regulatory site Ser540. A fifth eIF2B-e phosphorylation site, Ser466, can be phosphorylated by casein kinase I.

Small volumes of anti-EIF2B5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS614000 is an antibody targeting EIF2B5. Also known as Eukaryotic Translation Initiation Factor 2B, epsilon (eIF2B-e). Reactive in Human, Monkey, Mouse, Rat. Host: Rabbit.