Goat Anti-MLX Polyclonal Antibody – Cat# MBS614055

MLX Antibody – Goat Polyclonal

Name: MLX Antibody; Polyclonal; Goat; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS614055
Price: 1025 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS614055

MLX (Max-like protein X; also known as transcription factor-like protein 4) is a 30kD member of the Max-like bHLHZip family of proteins. It is widely expressed and is found in both cytoplasm and nucleus. MLX is a transcriptional repressor when dimerized with… More details

$1025 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

MLX, Polyclonal Antibody

Full Product Name

MLX (Max-like protein X, Transcription factor-like protein 4)

Product Gene Name

anti-MLX antibody

Product Synonym Names

Anti -MLX (Max-like protein X, Transcription factor-like protein 4)

Clonality

Polyclonal

Isotype

IgG

Host

Goat

Species Reactivity

Human

Form/Format

Supplied as a lyophilized powder from PBS with 5% trehalose. Reconstitute with 0.5ml PBS to a concentration of 0.2mg/ml.

Purity/Purification

Affinity Purified
Purified by human MLX affinity chromatography.

Specificity

Recognizes human MLX. Species sequence homology: 99% identical to mouse and canine MLX.

Preparation and Storage

Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

17,705 Da

Chromosome Location

Chromosome: 17; NC_000017.10 (40719078..40725221). Location: 17q21.1

3D Structure

ModBase 3D Structure for Q59G74

Immunogen

E. coli-derived recombinant human rhMLX (aa 197-298).

Hybridoma

Produced in a goat immunized with purified, E. coli-derived, recombinant human MLX
(rhMLX; aa 197-298)

Product Categories/Family for anti-MLX antibody

Antibodies; Abs to Proteins

Applications Tested/Suitable for anti-MLX antibody

ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC)

Application Notes for anti-MLX antibody

Suitable for use in Western blot, Immunocytochemistry and ELISA.
Dilution: Western blot: 0.1-0.2ug/ml. The detetction limit is ~2ng/lane under non-reducing and reducing conditions.
Immunocytochemistry: 10ug/ml will detect human MLX in serum stimulated W138 human fetal lung fibroblasts. Fix cells with PBS containing 4% paraformaldehyde for 20 minutes at RT and block with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at RT. After blocking, incubate cells with diluted primary antibody for 3 hours at RT
followed by NorthernLightsTM 557 donkey anti-goat lgG atRT for 1 hour. Between each step, wash cells with PBS containing 0.1% BSA.
Direct ELISA: 0.5-1.0ug/ml. The detection limit is ~0.3ng/well.

Other Notes

Small volumes of anti-MLX antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MLX purchase

MBS614055 is an antibody targeting MLX. Also known as MLX (Max-like protein X, Transcription factor-like protein 4). Reactive in Human. Host: Goat.

Additional Details

NCBI/Uniprot data below describe general gene information for MLX. It may not necessarily be applicable to this product.

NCBI GI #

38201614

NCBI GeneID

6945

NCBI Accession #

NP_937848.1

UniProt Primary Accession #

Q59G74

UniProt Related Accession #

Q59G74; Q9UH92

GenBank Nucleotide #

NM_198205.1

NCBI Official Full Name

max-like protein X isoform alpha

NCBI Official Synonym Full Names

MAX-like protein X

NCBI Official Symbol

MLX

NCBI Official Synonym Symbols

MAD7; MXD7; TCFL4; bHLHd13

UniProt Protein Name

Transcription factor-like protein 4 isoform alpha variant

Protein Family

Max-like protein

UniProt Entry Name

Q59G74_HUMAN

NCBI Protein Information

max-like protein X; BigMax protein; MAX-like bHLHZIP protein; transcription factor-like protein 4; class D basic helix-loop-helix protein 13

NCBI Summary for MLX

The product of this gene belongs to the family of basic helix-loop-helix leucine zipper (bHLH-Zip) transcription factors. These factors form heterodimers with Mad proteins and play a role in proliferation, determination and differentiation. This gene product may act to diversify Mad family function by its restricted association with a subset of the Mad family of transcriptional repressors, namely, Mad1 and Mad4. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq]

UniProt Comments for MLX

Sequence similarities: Contains 1 basic helix-loop-helix (bHLH) domain. RuleBase RU000367

Research Articles on MLX

1. These studies suggest a key role for MondoA:Mlx complexes in the adaptive transcriptional response to changes in extracellular glucose concentration and peripheral glucose uptake.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MLX antibody

Reference Product	PubMed Publications
MLXIPL antibody	>9 publications with MLX and MLXIPL
MNT antibody	>4 publications with MLX and MNT

Pathways associated with anti-MLX antibody

Products by Pathway	Pathway Diagram
ChREBP Activates Metabolic Gene Expression Pathway antibodies	ChREBP Activates Metabolic Gene Expression Pathway Diagram
Integration Of Energy Metabolism Pathway antibodies	Integration Of Energy Metabolism Pathway Diagram

Diseases associated with anti-MLX antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>51 publications with MLX and Neoplasms
Liver Diseases Antibodies	>50 publications with MLX and Liver Diseases
Adenocarcinoma Antibodies	>35 publications with MLX and Adenocarcinoma
Carcinoma, Hepatocellular Antibodies	>35 publications with MLX and Carcinoma, Hepatocellular
Hepatitis Antibodies	>15 publications with MLX and Hepatitis
Fatty Liver Antibodies	>12 publications with MLX and Fatty Liver
Gastrointestinal Diseases Antibodies	>9 publications with MLX and Gastrointestinal Diseases
Liver Neoplasms, Experimental Antibodies	>6 publications with MLX and Liver Neoplasms, Experimental
Necrosis Antibodies	>6 publications with MLX and Necrosis
Cardiovascular Diseases Antibodies	>6 publications with MLX and Cardiovascular Diseases

Organs/Tissues associated with anti-MLX antibody

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>165 publications with MLX and Liver
Blood Antibodies	>53 publications with MLX and Blood
Pancreas Antibodies	>36 publications with MLX and Pancreas
Kidney Antibodies	>32 publications with MLX and Kidney
Thyroid Antibodies	>20 publications with MLX and Thyroid
Intestine Antibodies	>20 publications with MLX and Intestine
Brain Antibodies	>11 publications with MLX and Brain
Lung Antibodies	>9 publications with MLX and Lung
Ear Antibodies	>7 publications with MLX and Ear
Embryonic Tissue Antibodies	>6 publications with MLX and Embryonic Tissue