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DCP1A Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6141481

MBS6141481 is an antibody targeting DCP1A. Also known as DCP1A (mRNA-decapping Enzyme 1A, Smad4-interacting Transcriptional Co-activator, Transcription Factor SMIF, SMIF, FLJ21691, HSA275986, Nbla00360) (Biotin). Reactive in Human. Host: Mouse. More details

$1155 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
DCP1A, Monoclonal Antibody
Full Product Name
DCP1A (mRNA-decapping Enzyme 1A, Smad4-interacting Transcriptional Co-activator, Transcription Factor SMIF, SMIF, FLJ21691, HSA275986, Nbla00360) (Biotin)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
3G4
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human DCP1A.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
56kDa
OMIM
607010
3D Structure
ModBase 3D Structure for Q9NPI6
Immunogen
Partial recombinant corresponding to aa186-286 from human DCP1A (NP_060873) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
STQLSNLGSTETLEEMPSGSQDKSAPSGHKHLTVEELFGTSLPKEQPAVVGLDSEEMERLPGDASQKEPNSFLPFPFEQLGGAPQSETLGVPSAAHHSVQ
Conjugate
Biotin
Applications Tested/Suitable for anti-DCP1A antibody
ELISA (EIA), Immunofluorescence (IF), Western Blot (WB)
Application Notes for anti-DCP1A antibody
IF: 10ug/ml
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-DCP1A antibody

Western Blot detection against Immunogen (37.11kD).
Mouse Anti-DCP1A Monoclonal Antibody – Cat# MBS6141481

Western Blot (WB) of anti-DCP1A antibody

DCP1A monoclonal antibody, Western Blot analysis of DCP1A expression in IMR-32.
Mouse Anti-DCP1A Monoclonal Antibody, Western Blot (WB) – Cat# MBS6141481

Immunofluorescence (IF) of anti-DCP1A antibody

Immunofluorescence of monoclonal antibody to DCP1A on HeLa cell. [antibody concentration 10ug/ml].
Mouse Anti-DCP1A Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS6141481

Testing Data of anti-DCP1A antibody

Detection limit for recombinant GST tagged DCP1A is ~0.1ng/ml as a capture antibody.
Mouse Anti-DCP1A Monoclonal Antibody, Testing Data – Cat# MBS6141481

Other Notes

Small volumes of anti-DCP1A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for DCP1A. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
mRNA-decapping enzyme 1A isoform a
NCBI Official Synonym Full Names
decapping mRNA 1A
NCBI Official Symbol
DCP1A
NCBI Official Synonym Symbols
SMIF; SMAD4IP1; HSA275986; Nbla00360
UniProt Protein Name
mRNA-decapping enzyme 1A
UniProt Synonym Protein Names
Smad4-interacting transcriptional co-activator; Transcription factor SMIF
Protein Family
UniProt Gene Name
DCP1A
UniProt Synonym Gene Names
SMIF
UniProt Entry Name
DCP1A_HUMAN
NCBI Protein Information
mRNA-decapping enzyme 1A

NCBI Summary for DCP1A

Decapping is a key step in general and regulated mRNA decay. The protein encoded by this gene is a decapping enzyme. This protein and another decapping enzyme form a decapping complex, which interacts with the nonsense-mediated decay factor hUpf1 and may be recruited to mRNAs containing premature termination codons. This protein also participates in the TGF-beta signaling pathway. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Feb 2014]
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UniProt Comments for DCP1A

DCP1A: a decapping enzyme. Plays a role in general and regulated mRNA decay. Apparently plays a role in the nonsense-mediated decay pathway that rids cells of mRNAs containing premature termination codons. May function as a transactivator of SMAD4 that participates in TGF-beta signaling.

Protein type: RNA processing; Transcription, coactivator/corepressor; EC 3.-.-.-; Hydrolase

Chromosomal Location of Human Ortholog: 3p21.1

Cellular Component: membrane; cytosol; nucleus

Molecular Function: identical protein binding; protein binding; hydrolase activity

Biological Process: mRNA catabolic process, nonsense-mediated decay; gene expression; mRNA catabolic process, deadenylation-dependent decay
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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