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ELF1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6141710

ELF1 is an E26 transformation-specific related transcription factor. The protein is primarily expressed in lymphoid cells and acts as both an enhancer and a repressor to regulate transcription of various genes. More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ELF1, Monoclonal Antibody
Full Product Name
ELF1 (ETS-related Transcription Factor Elf-1, E74-like Factor 1) (Biotin)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
3B7
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human ELF1.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
67kDa
OMIM
189973
3D Structure
ModBase 3D Structure for P32519
Immunogen
Partial recombinant corresponding to aa421-520 from human ELF1 (NP_758961) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
IQAPTQVPVVVSPRNQQLHTVTLQTVPLTTVIASTDPSAGTGSQKFILQAIPSSQPMTVLKENVMLQSQKAGSPPSIVLGPAQVQQVLTSNVQTICNGTV
Conjugate
Biotin
Applications Tested/Suitable for anti-ELF1 antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-ELF1 antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-ELF1 antibody

Western Blot detection against Immunogen (36.74kD).
Mouse Anti-ELF1 Monoclonal Antibody – Cat# MBS6141710

Western Blot (WB) of anti-ELF1 antibody

ELF1 monoclonal antibody, Western Blot analysis of ELF1 expression in 293.
Mouse Anti-ELF1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6141710

Testing Data of anti-ELF1 antibody

Detection limit for recombinant GST tagged ELF1 is ~1ng/ml as a capture antibody.
Mouse Anti-ELF1 Monoclonal Antibody, Testing Data – Cat# MBS6141710

Other Notes

Small volumes of anti-ELF1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ELF1 purchase

MBS6141710 is an antibody targeting ELF1. Also known as ELF1 (ETS-related Transcription Factor Elf-1, E74-like Factor 1) (Biotin). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for ELF1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
ETS-related transcription factor Elf-1 isoform a
NCBI Official Synonym Full Names
E74 like ETS transcription factor 1
NCBI Official Symbol
ELF1
NCBI Official Synonym Symbols
RIA1; EFTUD1
UniProt Protein Name
ETS-related transcription factor Elf-1
UniProt Synonym Protein Names
E74-like factor 1
UniProt Gene Name
ELF1
UniProt Entry Name
ELF1_HUMAN
NCBI Protein Information
ETS-related transcription factor Elf-1

NCBI Summary for ELF1

This gene encodes an E26 transformation-specific related transcription factor. The encoded protein is primarily expressed in lymphoid cells and acts as both an enhancer and a repressor to regulate transcription of various genes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2009]
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UniProt Comments for ELF1

Elf-1: Transcription factor that activates the LYN and BLK promoters. Appears to be required for the T-cell-receptor-mediated trans activation of HIV-2 gene expression. Binds specifically to two purine-rich motifs in the HIV-2 enhancer. Belongs to the ETS family.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 13q13

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; negative regulation of T cell receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; cell differentiation; regulation of cytokine production
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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