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KCNJ15 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6142574

Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. The encoded protein has a… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
KCNJ15, Monoclonal Antibody
Full Product Name
KCNJ15 (KCNJ14, ATP-sensitive Inward Rectifier Potassium Channel 15, Inward Rectifier K(+) Channel Kir1.3, Inward Rectifier K(+) Channel Kir4.2, Potassium Channel, Inwardly Rectifying Subfamily J Member 15, MGC13584) (Biotin)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
1B2
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human KCNJ15.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
3D Structure
ModBase 3D Structure for Q99712
Immunogen
Partial recombinant corresponding to aa290-355 from human KCNJ15 with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
TSAVCQSRTSYIPEEIYWGFEFVPVVSLSKNGKYVADFSQFEQIRKSPDCTFYCADSEKQQLEEKY
Conjugate
Biotin

Product Categories/Family for anti-KCNJ15 antibody

Applications Tested/Suitable for anti-KCNJ15 antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-KCNJ15 antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-KCNJ15 antibody

Western Blot detection against Immunogen (33kD) usingMBS649976.
Mouse Anti-KCNJ15 Monoclonal Antibody – Cat# MBS6142574

Western Blot (WB) of anti-KCNJ15 antibody

Western Blot analysis of KCNJ15 expression in transfected 293T cell line usingMBS649976. Lane 1: KCNJ15 transfected lysate (42.6kD). Lane 2: Non-transfected lysate.
Mouse Anti-KCNJ15 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6142574

Western Blot (WB) of anti-KCNJ15 antibody

Western Blot analysis of KCNJ15 over-expressed 293 cell line, cotransfected with KCNJ15 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed withMBS649976. GAPDH (36.1kD) used as specificity and loading control.
Mouse Anti-KCNJ15 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6142574

Testing Data of anti-KCNJ15 antibody

Detection limit for recombinant GST tagged KCNJ15 is 3ng/ml using MBS649976 as a capture antibody.
Mouse Anti-KCNJ15 Monoclonal Antibody, Testing Data – Cat# MBS6142574

Other Notes

Small volumes of anti-KCNJ15 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for KCNJ15 purchase

MBS6142574 is an antibody targeting KCNJ15. Also known as KCNJ15 (KCNJ14, ATP-sensitive Inward Rectifier Potassium Channel 15, Inward Rectifier K(+) Channel Kir1.3, Inward Rectifier K(+) Channel Kir4.2, Potassium Channel, Inwardly Rectifying Subfamily J Member 15, MGC13584) (Biotin). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for KCNJ15. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
ATP-sensitive inward rectifier potassium channel 15
NCBI Official Synonym Full Names
potassium inwardly rectifying channel subfamily J member 15
NCBI Official Symbol
KCNJ15
NCBI Official Synonym Symbols
IRKK; KIR1.3; KIR4.2
UniProt Protein Name
ATP-sensitive inward rectifier potassium channel 15
UniProt Synonym Protein Names
Inward rectifier K(+) channel Kir1.3; Inward rectifier K(+) channel Kir4.2; Potassium channel, inwardly rectifying subfamily J member 15
UniProt Gene Name
KCNJ15
UniProt Synonym Gene Names
KCNJ14
NCBI Protein Information
ATP-sensitive inward rectifier potassium channel 15

NCBI Summary for KCNJ15

Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. The encoded protein has a greater tendency to allow potassium to flow into a cell rather than out of a cell. Eight transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Feb 2013]
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UniProt Comments for KCNJ15

Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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