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NEK2 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6143143

MBS6143143 is an antibody targeting NEK2. Also known as NEK2 (Never in Mitosis A-related Kinase 2, NimA-related Protein Kinase 2, NEK2A, HSPK 21, HsPK21, NimA-like Protein Kinase 1, NLK1, Serine/Threonine Protein Kinase Nek2) (Biotin). Reactive in Human.… More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NEK2, Monoclonal Antibody
Full Product Name
NEK2 (Never in Mitosis A-related Kinase 2, NimA-related Protein Kinase 2, NEK2A, HSPK 21, HsPK21, NimA-like Protein Kinase 1, NLK1, Serine/Threonine Protein Kinase Nek2) (Biotin)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
2F6
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human NEK2.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Partial recombinant corresponding to aa331-445 from human NEK2 (AAH43502) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
EQELCVRERLAEDKLARAENLLKNYSLLKERKFLSLASNPELLNLPSSVIKKKVHFSGESKENIMRSENSESQLTSKSKCKDLKKRLHAAQLRAQALSDIEKNYQLKSRQILGMR
Conjugate
Biotin

Product Categories/Family for anti-NEK2 antibody

Applications Tested/Suitable for anti-NEK2 antibody
ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Immunoprecipitation (IP), Western Blot (WB)
Application Notes for anti-NEK2 antibody
IHC-P: 1ug/ml
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-NEK2 antibody

Western Blot detection against Immunogen (38.28kD).
Mouse Anti-NEK2 Monoclonal Antibody – Cat# MBS6143143

Western Blot (WB) of anti-NEK2 antibody

Western Blot analysis of NEK2 expression in transfected 293T cell line by NEK2 monoclonal antibody Lane 1: NEK2 transfected lysate (51.8kD). Lane 2: Non-transfected lysate
Mouse Anti-NEK2 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6143143

Immunohistochemistry (IHC) of anti-NEK2 antibody

Immunoperoxidase of monoclonal antibody to NEK2 on formalin-fixed paraffin-embedded human testis. [antibody concentration 1ug/ml]
Mouse Anti-NEK2 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6143143

Immunoprecipitation (IP) of anti-NEK2 antibody

Immunoprecipitation of NEK2 transfected lysate using NEK2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with NEK2 rabbit polyclonal antibody.
Mouse Anti-NEK2 Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS6143143

Testing Data of anti-NEK2 antibody

Detection limit for recombinant GST tagged NEK2 is ~0.3ng/ml as a capture antibody.
Mouse Anti-NEK2 Monoclonal Antibody, Testing Data – Cat# MBS6143143

Other Notes

Small volumes of anti-NEK2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NEK2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Related Accession #
NCBI Official Full Name
Homo sapiens NIMA (never in mitosis gene a)-related kinase 2, mRNA
NCBI Official Synonym Full Names
NIMA related kinase 2
NCBI Official Symbol
NEK2
NCBI Official Synonym Symbols
NLK1; RP67; NEK2A; HsPK21; PPP1R111
NCBI Protein Information
serine/threonine-protein kinase Nek2

NCBI Summary for NEK2

This gene encodes a serine/threonine-protein kinase that is involved in mitotic regulation. This protein is localized to the centrosome, and undetectable during G1 phase, but accumulates progressively throughout the S phase, reaching maximal levels in late G2 phase. Alternatively spliced transcript variants encoding different isoforms with distinct C-termini have been noted for this gene. [provided by RefSeq, Feb 2011]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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