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NMI Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6143208

MBS6143208 is an antibody targeting NMI. Also known as NMI (N-myc-interactor, Nmi, N-myc and STAT Interactor) (Biotin). Reactive in Human. Host: Mouse. More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NMI, Monoclonal Antibody
Full Product Name
NMI (N-myc-interactor, Nmi, N-myc and STAT Interactor) (Biotin)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
9D8
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human NMI.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
35,057 Da
Immunogen
Partial recombinant corresponding to aa1-101 from NMI (AAH01268) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MEADKDDTQQILKEHSPDEFIKDEQNKGLIDEITKKNIQLKKEIQKLETELQEATKEFQIKEDIPETKMKFLSVETPENDSQLSNISCSFQVSSKVPYEI
Conjugate
Biotin
Applications Tested/Suitable for anti-NMI antibody
ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes for anti-NMI antibody
IHC-P: 3ug/ml
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-NMI antibody

Western Blot detection against Immunogen (37.11kD).
Mouse Anti-NMI Monoclonal Antibody – Cat# MBS6143208

Western Blot (WB) of anti-NMI antibody

NMI monoclonal antibody Western Blot analysis of NMI expression in HeLa.
Mouse Anti-NMI Monoclonal Antibody, Western Blot (WB) – Cat# MBS6143208

Western Blot (WB) of anti-NMI antibody

Western Blot analysis of NMI expression in transfected 293T cell line by NMI monoclonal antibody. Lane 1: NMI transfected lysate (35.1kD). Lane 2: Non-transfected lysate.
Mouse Anti-NMI Monoclonal Antibody, Western Blot (WB) – Cat# MBS6143208

Immunohistochemistry (IHC) of anti-NMI antibody

Immunoperoxidase of monoclonal antibody to NMI on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3ug/ml]
Mouse Anti-NMI Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6143208

Testing Data of anti-NMI antibody

Detection limit for recombinant GST tagged NMI is ~0.03ng/ml as a capture antibody.
Mouse Anti-NMI Monoclonal Antibody, Testing Data – Cat# MBS6143208

Western Blot (WB) of anti-NMI antibody

Western blot analysis of NMI over-expressed 293 cell line, cotransfected with NMI Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with NMI monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.
Mouse Anti-NMI Monoclonal Antibody, Western Blot (WB) – Cat# MBS6143208

Other Notes

Small volumes of anti-NMI antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NMI. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Homo sapiens N-myc (and STAT) interactor, mRNA
NCBI Official Synonym Full Names
N-myc and STAT interactor
NCBI Official Symbol
NMI
Protein Family
NCBI Protein Information
N-myc-interactor

NCBI Summary for NMI

NMYC interactor (NMI) encodes a protein that interacts with NMYC and CMYC (two members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH-Zip motif. The NMI protein also interacts with all STATs except STAT2 and augments STAT-mediated transcription in response to cytokines IL2 and IFN-gamma. The NMI mRNA has low expression levels in all human fetal and adult tissues tested except brain and has high expression in cancer cell line-myeloid leukemias. [provided by RefSeq, Jul 2008]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-NMI antibody

 Products by Pathway  Pathway Diagram
 IL-2 Signaling Pathway antibodies  IL-2 Signaling Pathway Diagram
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