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PIGQ Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6143553

MBS6143553 is an antibody targeting PIGQ. Also known as PIGQ (Phosphatidylinositol N-acetylglucosaminyltransferase Subunit Q, N-acetylglucosamyl Transferase Component GPI1, Phosphatidylinositol-glycan Biosynthesis Class Q Protein, PIG-Q, GPI1, c407A10.1,… More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PIGQ, Monoclonal Antibody
Full Product Name
PIGQ (Phosphatidylinositol N-acetylglucosaminyltransferase Subunit Q, N-acetylglucosamyl Transferase Component GPI1, Phosphatidylinositol-glycan Biosynthesis Class Q Protein, PIG-Q, GPI1, c407A10.1, hGPI1, MGC12693) (Biotin)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
2B7
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human PIGQ.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
84kDa
OMIM
605754
3D Structure
ModBase 3D Structure for Q9BRB3
Immunogen
Partial recombinant corresponding to aa661-759 from PIGQ (NP_683721) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
HCPMPTLCTQVQRVRPPQQPQVEGWSPWGLPSGSALAVGVEGPCQDEPPSPRHPLAPSAEQHPASGGLKQSLTPVPSGPGPSLPEPHGVYLRMFPGEV
Conjugate
Biotin
Applications Tested/Suitable for anti-PIGQ antibody
ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes for anti-PIGQ antibody
IHC-P: 4ug/ml
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-PIGQ antibody

Western Blot detection against Immunogen (36.89kD).
Mouse Anti-PIGQ Monoclonal Antibody – Cat# MBS6143553

Western Blot (WB) of anti-PIGQ antibody

Western Blot analysis of PIGQ expression in transfected 293T cell line by PIGQ monoclonal antibody. Lane 1: PIGQ transfected lysate (84.1kD). Lane 2: Non-transfected lysate.
Mouse Anti-PIGQ Monoclonal Antibody, Western Blot (WB) – Cat# MBS6143553

Immunohistochemistry (IHC) of anti-PIGQ antibody

Immunoperoxidase of monoclonal antibody to PIGQ on formalin-fixed paraffin-embedded human placenta. [antibody concentration 4ug/ml]
Mouse Anti-PIGQ Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6143553

Testing Data of anti-PIGQ antibody

Detection limit for recombinant GST tagged PIGQ is ~0.1ng/ml as a capture antibody.
Mouse Anti-PIGQ Monoclonal Antibody, Testing Data – Cat# MBS6143553

Other Notes

Small volumes of anti-PIGQ antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PIGQ. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
phosphatidylinositol N-acetylglucosaminyltransferase subunit Q isoform 1
NCBI Official Synonym Full Names
phosphatidylinositol glycan anchor biosynthesis class Q
NCBI Official Symbol
PIGQ
NCBI Official Synonym Symbols
GPI1; c407A10.1
UniProt Protein Name
Phosphatidylinositol N-acetylglucosaminyltransferase subunit Q
UniProt Synonym Protein Names
N-acetylglucosamyl transferase component GPI1; Phosphatidylinositol-glycan biosynthesis class Q protein; PIG-Q
UniProt Gene Name
PIGQ
UniProt Synonym Gene Names
GPI1; PIG-Q
UniProt Entry Name
PIGQ_HUMAN
NCBI Protein Information
phosphatidylinositol N-acetylglucosaminyltransferase subunit Q

NCBI Summary for PIGQ

This gene is involved in the first step in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This gene encodes a N-acetylglucosaminyl transferase component that is part of the complex that catalyzes transfer of N-acetylglucosamine (GlcNAc) from UDP-GlcNAc to phosphatidylinositol (PI). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2012]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PIGQ antibody

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