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RASEF Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6143935

MBS6143935 is an antibody targeting RASEF. Also known as RASEF (Ras and EF-hand Domain-containing Protein, Ras-related Protein Rab-45, RAB45) (Biotin). Reactive in Human. Host: Mouse. More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
RASEF, Monoclonal Antibody
Full Product Name
RASEF (Ras and EF-hand Domain-containing Protein, Ras-related Protein Rab-45, RAB45) (Biotin)
Product Gene Name
Product Synonym Names
FLJ31614
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
2D5
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human RASEF.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Partial recombinant protein corresponding to aa631-740 from human RASEF (AAH23566) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
NIREWVDMIEDAAHETVPIMLVGNKADIRDTAATEGQKCVPGHFGEKLAMTYGALFCETSAKDGSNIVEAVLHLAREVKKRTDKDDSRSITNLTGTNSKKSPQMKNCCNG
Conjugate
Biotin

Product Categories/Family for anti-RASEF antibody

Applications Tested/Suitable for anti-RASEF antibody
ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes for anti-RASEF antibody
IHC-P: 3ug/ml
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-RASEF antibody

Western Blot detection against Immunogen (38.1kD).
Mouse Anti-RASEF Monoclonal Antibody – Cat# MBS6143935

Western Blot (WB) of anti-RASEF antibody

RASEF monoclonal antibody, Western Blot analysis of RASEF expression in K-562.
Mouse Anti-RASEF Monoclonal Antibody, Western Blot (WB) – Cat# MBS6143935

Immunohistochemistry (IHC) of anti-RASEF antibody

Immunoperoxidase of monoclonal antibody to RASEF on formalin-fixed paraffin-embedded human adrenal gland. [antibody concentration 3ug/ml].
Mouse Anti-RASEF Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6143935

Testing Data of anti-RASEF antibody

Detection limit for recombinant GST tagged RASEF is ~0.3ng/ml as a capture antibody.
Mouse Anti-RASEF Monoclonal Antibody, Testing Data – Cat# MBS6143935

Other Notes

Small volumes of anti-RASEF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for RASEF. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Related Accession #
NCBI Official Full Name
Homo sapiens RAS and EF-hand domain containing, mRNA
NCBI Official Synonym Full Names
RAS and EF-hand domain containing
NCBI Official Symbol
RASEF
NCBI Official Synonym Symbols
RAB45
NCBI Protein Information
ras and EF-hand domain-containing protein

NCBI Summary for RASEF

This gene is a member of the Rab family of GTPases that are involved in regulation of membrane traffic. The encoded protein contains an N-terminal EF-hand domain, a coiled-coil motif and a C-terminal Rab domain. A potential role as tumor suppressor has been indicated for this gene. [provided by RefSeq, Nov 2012]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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