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Transcription Factor Sp7 (SP7) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6144546

MBS6144546 is an antibody targeting SP7. Also known as Transcription Factor Sp7 (SP7, Zinc Finger Protein Osterix, OSX) (Biotin). Reactive in Human. Host: Mouse. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
Transcription Factor Sp7, Monoclonal Antibody
Full Product Name
Transcription Factor Sp7 (SP7, Zinc Finger Protein Osterix, OSX) (Biotin)
Product Gene Name
Product Synonym Names
OI11; OI12
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
2G6
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human SP7.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
45kDa
OMIM
AF466179 mRNA
3D Structure
ModBase 3D Structure for Q8TDD2
Immunogen
Partial recombinant corresponding to aa87-163 from human SP7 (NP_690599) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
GYANDYPPFSHSFPGPTGTQDPGLLVPKGHSSSDCLPSVYTSLDMTHPYGSWYKAGIHAGISPGPGNTPTPWWDMH
Conjugate
Biotin

Product Categories/Family for anti-SP7 antibody

Applications Tested/Suitable for anti-SP7 antibody
ELISA (EIA), Immunofluorescence (IF), Western Blot (WB)
Application Notes for anti-SP7 antibody
IF: 10ug/ml
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-SP7 antibody

Western Blot detection against Immunogen (34.47kD).
Mouse Anti-SP7 Monoclonal Antibody – Cat# MBS6144546

Immunofluorescence (IF) of anti-SP7 antibody

Immunofluorescence of monoclonal antibody to SP7 on HeLa cell. [antibody concentration 10ug/ml].
Mouse Anti-SP7 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS6144546

Testing Data of anti-SP7 antibody

Detection limit for recombinant GST tagged SP7 is ~1ng/ml as a capture antibody.
Mouse Anti-SP7 Monoclonal Antibody, Testing Data – Cat# MBS6144546

Other Notes

Small volumes of anti-SP7 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SP7. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
transcription factor Sp7 isoform a
NCBI Official Synonym Full Names
Sp7 transcription factor
NCBI Official Symbol
SP7
NCBI Official Synonym Symbols
OSX; OI11; OI12; osterix
UniProt Protein Name
Transcription factor Sp7
UniProt Synonym Protein Names
Zinc finger protein osterix
Protein Family
UniProt Gene Name
SP7
UniProt Synonym Gene Names
OSX
NCBI Protein Information
transcription factor Sp7

NCBI Summary for SP7

This gene encodes a member of the Sp subfamily of Sp/XKLF transcription factors. Sp family proteins are sequence-specific DNA-binding proteins characterized by an amino-terminal trans-activation domain and three carboxy-terminal zinc finger motifs. This protein is a bone specific transcription factor and is required for osteoblast differentiation and bone formation.[provided by RefSeq, Jul 2010]
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UniProt Comments for SP7

Transcriptional activator essential for osteoblast differentiation. Binds to SP1 and EKLF consensus sequences and to other G/C-rich sequences ().

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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