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SPDEF Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6144553

MBS6144553 is an antibody targeting SPDEF. Also known as SPDEF (SAM Pointed Domain-containing Ets Transcription Factor, Prostate Epithelium-specific Ets Transcription Factor, Prostate-specific Ets, Prostate-derived Ets Factor, PDEF, PSE) (Biotin). Reactive in… More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
SPDEF, Monoclonal Antibody
Full Product Name
SPDEF (SAM Pointed Domain-containing Ets Transcription Factor, Prostate Epithelium-specific Ets Transcription Factor, Prostate-specific Ets, Prostate-derived Ets Factor, PDEF, PSE) (Biotin)
Product Gene Name
Product Synonym Names
bA375E1.3; RP11-375E1__A.3
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
4A5
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human SPDEF.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Partial recombinant corresponding to aa92-201 from SPDEF (NP_036523) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
QCPVIDSQAPAGSLDLVPGGLTLEEHSLEQVQSMVVGEVLKDIETACKLLNITADPMDWSPSNVQKWLLWTEHQYRLPPMGKAFQELAGKELCAMSEEQFRQRSPLGGD*
Conjugate
Biotin
Applications Tested/Suitable for anti-SPDEF antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-SPDEF antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-SPDEF antibody

Western Blot detection against Immunogen (38.1kD).
Mouse Anti-SPDEF Monoclonal Antibody – Cat# MBS6144553

Western Blot (WB) of anti-SPDEF antibody

Western Blot analysis of SPDEF expression in transfected 293T cell line by SPDEF monoclonal antibody. Lane 1: SPDEF transfected lysate (37.518kD). Lane 2: Non-transfected lysate.
Mouse Anti-SPDEF Monoclonal Antibody, Western Blot (WB) – Cat# MBS6144553

Other Notes

Small volumes of anti-SPDEF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SPDEF. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
Homo sapiens SAM pointed domain containing ETS transcription factor (SPDEF), transcript variant 1, mRNA
NCBI Official Synonym Full Names
SAM pointed domain containing ETS transcription factor
NCBI Official Symbol
SPDEF
NCBI Official Synonym Symbols
PDEF; bA375E1.3
NCBI Protein Information
SAM pointed domain-containing Ets transcription factor

NCBI Summary for SPDEF

The protein encoded by this gene belongs to the ETS family of transcription factors. It is highly expressed in the prostate epithelial cells, and functions as an androgen-independent transactivator of prostate-specific antigen (PSA) promoter. Higher expression of this protein has also been reported in brain, breast, lung and ovarian tumors, compared to the corresponding normal tissues, and it shows better tumor-association than other cancer-associated molecules, making it a more suitable target for developing specific cancer therapies. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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