• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

SQSTM1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6144576

MBS6144576 is an antibody targeting SQSTM1. Also known as SQSTM1 (Sequestosome-1, EBI3-associated Protein of 60kD, EBIAP, p60, ORCA, OSIL, Phosphotyrosine-independent Ligand for the Lck SH2 Domain of 62kD, Ubiquitin-binding Protein p62) (Biotin). Reactive in… More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
SQSTM1, Monoclonal Antibody
Full Product Name
SQSTM1 (Sequestosome-1, EBI3-associated Protein of 60kD, EBIAP, p60, ORCA, OSIL, Phosphotyrosine-independent Ligand for the Lck SH2 Domain of 62kD, Ubiquitin-binding Protein p62) (Biotin)
Product Gene Name
Product Synonym Names
A170; p60; p62; p62B; PDB3; ZIP3
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
2C11
Host
Mouse
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human SQSTM1. Species Crossreactivity: mouse
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
38,629 Da
Immunogen
Full length recombinant protein corresponding to aa1-440 from human SQSTM1 with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MASLTVKAYLLGKEDAAREIRRFSFCCSPEPEAEAEAAAGPGPCERLLSRVAALFPALRPGGFQAHYRDEDGDLVAFSSDEELTMAMSYVKDDIFRIYIKEKKECRRDHRPPCAQEAPRNMVHPNVICDGCNGPVVGTRYKCSVCPDYDLCSVCEGKGLHRGHTKLAFPSPFGHLSEGFSHSRWLRKVKHGHFGWPGWEMGPPGNWSPRPPRAGEARPGPTAESASGPSEDPSVNFLKNVGESVAAALSPLGIEVDIDVEHGGKRSRLTPVSPESSSTEEKSSSQPSSCCSDPSKPGGNVEGATQSLAEQMRKIALESEGRPEEQMESDNCSGGDDDWTHLSSKEVDPSTGELQSLQMPESEGPSSLDPSQEGPTGLKEAALYPHLPPEADPRLIESLSQMLSMGFSDEGGWLTRLLQTKNYDIGAALDTIQYSKHPPPL
Conjugate
Biotin

Product Categories/Family for anti-SQSTM1 antibody

Applications Tested/Suitable for anti-SQSTM1 antibody
ELISA (EIA), Immunofluorescence (IF), Western Blot (WB)
Application Notes for anti-SQSTM1 antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-SQSTM1 antibody

Western Blot detection against immunogen using 133821 (74.51kD).
Mouse Anti-SQSTM1 Monoclonal Antibody – Cat# MBS6144576

Testing Data of anti-SQSTM1 antibody

Detection limit is ~0.03ng/ml using 133821 as a capture antibody.
Mouse Anti-SQSTM1 Monoclonal Antibody, Testing Data – Cat# MBS6144576

Immunofluorescence (IF) of anti-SQSTM1 antibody

Immunofluorescence to SQSTM1 on HeLa cell using 133821 (10ug/ml).
Mouse Anti-SQSTM1 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS6144576

Product References and Citations for anti-SQSTM1 antibody

1. Host Cell Autophagy Modulates Early Stages of Adenovirus Infections in Airway Epithelial Cells. Zeng X, Carlin CR.J Virol. 2012 Dec 12. 2. Astrocyte-Specific Overexpression of Nrf2 Delays Motor Pathology and Synuclein Aggregation throughout the CNS in the Alpha-Synuclein Mutant (A53T) Mouse Model. Gan L, Vargas MR, Johnson DA, Johnson JA.J Neurosci. 2012 Dec 5;32(49):17775-87. doi: 10.1523/JNEUROSCI.3049-12.2012. 3. Hyperactivation of the mammalian degenerin MDEG promotes caspase-8 activation and apoptosis. Pan JA, Fan Y, Gandhirajan RK, Madesh M, Zong WX.J Biol Chem. 2012 Dec 13. 4. Defective autophagy and mTORC1 signaling in myotubularin null mice. Fetalvero KM, Yu Y, Goetschkes M, Liang G, Valdez RA, Gould T, Triantafellow E, Bergling S, Loureiro J, Eash J, Lin V, Porter JA, Finan PM, Walsh K, Yang Y, Mao X, Murphy LO.Mol Cell Biol. 2012 Oct 29. 5. Recombinant protein rVP1 upregulates BECN1-independent autophagy, MAPK1/3 phosphorylation and MMP9 activity via WIPI1/WIPI2 to promote macrophage migration. Liao CC, Ho MY, Liang SM, Liang CMAutophagy. 2013 Jan;9(1):5-19. doi: 10.4161/auto.22379. Epub 2012 Oct 10. 6. (-)-Epigallocatechin-3-Gallate Induces Non-Apoptotic Cell Death in Human Cancer Cells via ROS-Mediated Lysosomal Membrane Permeabilization. Zhang Y, Yang ND, Zhou F, Shen T, Duan T, Zhou J, Shi Y, Zhu XQ, Shen HM.PLoS One. 2012;7(10):e46749. doi: 10.1371/journal.pone.0046749. Epub 2012 Oct 8. 7. West Nile Virus (WNV) Replication Is Independent of Autophagy in Mammalian Cells. Vandergaast R, Fredericksen BL.PLoS One. 2012;7(9):e45800. doi: 10.1371/journal.pone.0045800. Epub 2012 Sep 21. 8. Rotenone Inhibits Autophagic Flux Prior to Inducing Cell Death. Mader BJ, Pivtoraiko VN, Flippo HM, Klocke BJ, Roth KA, Mangieri LR, ShackaJJ.ACS Chem. Neurosci. DOI: 10.1021/cn300145z 9. Tissue Specific Induction of p62/Sqstm1 by Farnesoid X Receptor. Williams JA, Thomas AM, Li G, Kong B, Zhan L, Inaba Y, Xie W, Ding WX, Guo GL.PLoS One. 2012;7(8):e43961. Epub 2012 Aug 27. 10. Sorafenib has potent anti-tumor activity in multiple myeloma in vitro, ex vivo and in vivo, in the 5T33MM mouse model. Kharaziha P, De Raeve H, Fristedt C, Li Q, Gruber A, Johnsson P, Kokaraki G, Panzar M, Laane E, Osterborg A, Zhivotovsky B, Jernberg-Wiklund H, Grander D, Celsing F, Bjorkholm M, Vanderkerken K, Panaretakis T.Cancer Res. 2012 Sep 4. 11. Genetic Ablation of Nrf2/Antioxidant Response Pathway in Alexander Disease Mice Reduces Hippocampal Gliosis but Does Not Impact Survival. Hagemann TL, Jobe EM, Messing A.PLoS One. 2012;7(5):e37304. Epub 2012 May 31. 12. MAP1B Interaction with the FW Domain of the Autophagic Receptor Nbr1 Facilitates Its Association to the Microtubule Network. Marchbank K, Waters S, Roberts RG, Solomon E, Whitehouse CA.Int J Cell Biol. 2012;2012:208014. Epub 2012 May 10. 13. Liver-specific loss of atg5 causes persistent activation of nrf2 and protects against acetaminophen-induced liver injury. Ni HM, Boggess N, McGill MR, Lebofsky M, Borude P, Apte U, Jaeschke H, Ding WX.Toxicol Sci. 2012 Jun;127(2):438-50. Epub 2012 Apr 5. 14. Chronic Activation of mTOR Complex 1 Is Sufficient to Cause Hepatocellular Carcinoma in Mice. Menon S, Yecies JL, Zhang HH, Howell JJ, Nicholatos J, Harputlugil E, Bronson RT, Kwiatkowski DJ, Manning BD.Sci Signal. 2012 Mar 27;5(217):ra24. 15. Progressive neuronal inclusion formation and axonal degeneration in CHMP2B mutant transgenic mice. Ghazi-Noori S, Froud KE, Mizielinska S, Powell C, Smidak M, Fernandez de Marco M, O'Malley C, Farmer M, Parkinson N, Fisher EM, Asante EA, Brandner S, Collinge J, Isaacs AM.Brain. 2012 Mar;135(Pt 3):819-32. 16. A centronuclear myopathy-dynamin 2 mutation impairs autophagy in mice. Durieux AC, Vassilopoulos S, Laine J, Fraysse B, Brinas L, Prudhon B, Castells J, Freyssenet D, Bonne G, Guicheney P, Bitoun M.Traffic. 2012 Feb 28. doi: 10.1111/j.1600-0854.2012.01348.x. 17. Targeting of distinct signaling cascades and cancer-associated fibroblasts define the efficacy of Sorafenib against prostate cancer cells. Kharaziha P, Rodriguez P, Li Q, Rundqvist H, Bjorklund AC, Augsten M, Ullen A, Egevad L, Wiklund P, Nilsson S, Kroemer G, Grander D, Panaretakis T.Cell Death Dis. 2012 Jan 26;3:e262. doi: 10.1038/cddis.2012.1. 18. Class III PI3K Vps34 plays an essential role in autophagy and in heart and liver function. Jaber N, Dou Z, Chen JS, Catanzaro J, Jiang YP, Ballou LM, Selinger E, Ouyang X, Lin RZ, Zhang J, Zong WX.Proc Natl Acad Sci U S A. 2012 Feb 7;109(6):2003-8. Epub 2012 Jan 23. 19. Cathepsin Cleavage of Sirtuin-1 in Endothelial Progenitor Cells Mediates Stress-Induced Premature Senescence. Chen J, Xavier S, Moskowitz-Kassai E, Chen R, Lu CY, Sanduski K, Spes A, Turk B, Goligorsky MS.Am J Pathol. 2012 Jan 9. 20. Autophagic degradation of tau in primary neurons and its enhancement by trehalose. Kruger U, Wang Y, Kumar S, Mandelkow EM.Neurobiol Aging. 2011 Dec 12. 21. Mutations in UBQLN2 cause dominant X-linked juvenile and adult-onset ALS and ALS/dementia. Deng HX, Chen W, Hong ST, Boycott KM, Gorrie GH, Siddique N, Yang Y, Fecto F, Shi Y, Zhai H, Jiang H, Hirano M, Rampersaud E, Jansen GH, Donkervoort S, Bigio EH, Brooks BR, Ajroud K, Sufit RL, Haines JL, Mugnaini E, Pericak-Vance MA, Siddique T.Nature. 2011 Aug 21;477(7363):211-5. doi: 10.1038/nature10353. 22. Modulation of pediatric brain tumor autophagy and chemosensitivity. Levy JM, Thorburn A.J Neurooncol. 2011 Aug 13. 23. Autophagy promotes T-cell survival through degradation of proteins of the cell death machinery. Kovacs JR, Li C, Yang Q, Li G, Garcia IG, Ju S, Roodman DG, Windle JJ, Zhang X, Lu B.Cell Death Differ. 2011 Jun 10. doi: 10.1038/cdd.2011.78. 24. Pancreatic cancers require autophagy for tumor growth. Yang S, Wang X, Contino G, Liesa M, Sahin E, Ying H, Bause A, Li Y, Stommel JM, Dell'antonio G, Mautner J, Tonon G, Haigis M, Shirihai OS, Doglioni C, Bardeesy N, Kimmelman AC.Genes Dev. 2011 Apr 1;25(7):717-29. Epub 2011 Mar 15. 25. Neighbor of Brca1 gene (Nbr1) functions as a negative regulator of postnatal osteoblastic bone formation and p38 MAPK activity. Whitehouse CA, Waters S, Marchbank K, Horner A, McGowan NW, V Jovanovic J, Xavier GM, Kashima TG, Cobourne MT, Richards GO, Sharpe PT, Skerry TM, Grigoriadis AE, Solomon E.Proc Natl Acad Sci U S A. 2010 Jun 29. 26. 14-3-3Tau regulates Beclin 1 and is required for autophagy. Wang B, Ling S, Lin WC.PLoS One. 2010 Apr 29;5(4):e10409. 27. In Vivo Requirement for Atg5 in Antigen Presentation by Dendritic Cells. Lee HK, Mattei LM, Steinberg BE, Alberts P, Lee YH, Chervonsky A, Mizushima N, Grinstein S, Iwasaki A.Immunity. 2010 Feb 26;32(2):227-39. Epub 2010 Feb 18. 28. Dual role of 3-methyladenine in modulation of autophagy via different temporal patterns of inhibition on class I and III phosphoinositide 3-kinase. Wu YT, Tan HL, Shui G, Bauvy C, Huang Q, Wenk MR, Ong CN, Codogno P, Shen HM.J Biol Chem. 2010 Apr 2;285(14):10850-61. Epub 2010 Feb 1. 29. Spred2 interaction with the late endosomal protein NBR1 down-regulates fibroblast growth factor receptor signaling. Mardakheh FK, Yekezare M, Machesky LM, Heath JK.J Cell Biol. 2009 Oct 12. 30. Defective autophagy in neurons and astrocytes from mice deficient in PI(3,5)P2. Ferguson CJ, Lenk GM, Meisler MH.Hum Mol Genet. 2009 Dec 15;18(24):4868-78. Epub 2009 Sep 29. 31. Sequestosome 1/p62 links familial ALS mutant SOD1 to LC3 via an ubiquitin-independent mechanism. Gal J, Strom AL, Kwinter DM, Kilty R, Zhang J, Shi P, Fu W, Wooten MW, Zhu H.J Neurochem. 2009 Nov;111(4):1062-73. Epub 2009 Sep 18. 32. Monitoring autophagic degradation of p62/SQSTM1. Bjorkoy G, Lamark T, Pankiv S, Overvatn A, Brech A, Johansen T.Methods Enzymol. 2009;452:181-97. 33. Loss of Autophagy Diminishes Pancreatic ?] Cell Mass and Function with Resultant Hyperglycemia. Jung HS, Chung KW, Won Kim J, Kim J, Komatsu M, Tanaka K, Nguyen YH, Kang TM, Yoon KH, Kim JW, Jeong YT, Han MS, Lee MK, Kim KW, Shin J, Lee MS.Cell Metab. 2008 Oct;8(4):318-24.

Other Notes

Small volumes of anti-SQSTM1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SQSTM1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Homo sapiens sequestosome 1, mRNA
NCBI Official Synonym Full Names
sequestosome 1
NCBI Official Symbol
SQSTM1
NCBI Official Synonym Symbols
p60; p62; A170; OSIL; PDB3; ZIP3; p62B; FTDALS3
Protein Family
NCBI Protein Information
sequestosome-1

NCBI Summary for SQSTM1

This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. [provided by RefSeq, Mar 2009]
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000