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MED21 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6144681

MBS6144681 is an antibody targeting MED21. Also known as MED21 (Mediator of RNA Polymerase II Transcription Subunit 21, Mediator Complex Subunit 21, RNA Polymerase II Holoenzyme Component SRB7, RNAPII Complex Component SRB7, hSrb7, SRB7, SURB7) (Biotin).… More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MED21, Monoclonal Antibody
Full Product Name
MED21 (Mediator of RNA Polymerase II Transcription Subunit 21, Mediator Complex Subunit 21, RNA Polymerase II Holoenzyme Component SRB7, RNAPII Complex Component SRB7, hSrb7, SRB7, SURB7) (Biotin)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
3E9
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human SURB7.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
15,564 Da
Immunogen
Full length recombinant corresponding to aa1-144 from SURB7 (AAH08380) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MADRLTQLQDAVNSLADQFCNAIGVLQQCGPPASFNNIQTAINKDQPANPTEEYAQLFAALIARTAKDIDVLIDSLPSEESTAALQAASLYKLEEENHEAATCLEDVVYRGDMLLEKIQSALADIAQSQLKTRSGTHSQSLPDS
Conjugate
Biotin

Product Categories/Family for anti-MED21 antibody

Applications Tested/Suitable for anti-MED21 antibody
ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-MED21 antibody
IF: 10ug/ml
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-MED21 antibody

Western Blot detection against Immunogen (41.58kD).
Mouse Anti-MED21 Monoclonal Antibody – Cat# MBS6144681

Western Blot (WB) of anti-MED21 antibody

SURB7 monoclonal antibody Western Blot analysis of SURB7 expression in HeLa.
Mouse Anti-MED21 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6144681

Immunohistochemistry (IHC) of anti-MED21 antibody

Immunoperoxidase of monoclonal antibody to SURB7 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 1.2ug/ml]
Mouse Anti-MED21 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6144681

Immunofluorescence (IF) of anti-MED21 antibody

Immunofluorescence of monoclonal antibody to SURB7 on HeLa cell. [antibody concentration 10ug/ml
Mouse Anti-MED21 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS6144681

Testing Data of anti-MED21 antibody

Detection limit for recombinant GST tagged SURB7 is ~0.03ng/ml as a capture antibody.
Mouse Anti-MED21 Monoclonal Antibody, Testing Data – Cat# MBS6144681

Other Notes

Small volumes of anti-MED21 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MED21. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Homo sapiens mediator complex subunit 21, mRNA
NCBI Official Synonym Full Names
mediator complex subunit 21
NCBI Official Symbol
MED21
NCBI Official Synonym Symbols
SRB7; SURB7; hSrb7
NCBI Protein Information
mediator of RNA polymerase II transcription subunit 21

NCBI Summary for MED21

This gene encodes a member of the mediator complex subunit 21 family. The encoded protein interacts with the human RNA polymerase II holoenzyme and is involved in transcriptional regulation of RNA polymerase II transcribed genes. A pseudogene of this gene is located on chromosome 8. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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