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Symplekin (SYMPK) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6144689

MBS6144689 is an antibody targeting SYMPK. Also known as Symplekin (SYMPK, SPK, SYM) (Biotin). Reactive in Human. Host: Mouse. More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
PDF
Testing Data View PDF
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Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Symplekin (SYMPK), Monoclonal Antibody
Full Product Name
Symplekin (SYMPK, SPK, SYM) (Biotin)
Product Gene Name
Product Synonym Names
FLJ27092
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
4C2
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human SYMPK.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Full length recombinant corresponding to aa1-534 from SYMPK (AAH30214) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MASGSGDSVTRRSVASQFFTQEEGPGIDGMTTSERVVDLLNQAALITNDSKITVLKQVQELIINKDPTLLDNFLDEIIAFQADKSIEVRKFVIGFIEEACKRDIELLLKLIANLNMLLRDENVNVVKKAILTMTQLYKVALQWMVKSRVISELQEACWDMVSAMAGDIILLLDSDNDGIRTHAIKFVEGLIVTLSPRMADSEIPRRQEHDISLDRIPRDHPYIQYNVLWEEGKAALEQLLKFMVHPAISSINLTT
Conjugate
Biotin

Product Categories/Family for anti-SYMPK antibody

Applications Tested/Suitable for anti-SYMPK antibody
ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-SYMPK antibody
IF: 10ug/ml
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-SYMPK antibody

Western Blot detection against Immunogen (84.74kD).
Mouse Anti-SYMPK Monoclonal Antibody – Cat# MBS6144689

Western Blot (WB) of anti-SYMPK antibody

SYMPK monoclonal antibody. Western Blot analysis of SYMPK expression in COLO 320 HSR.
Mouse Anti-SYMPK Monoclonal Antibody, Western Blot (WB) – Cat# MBS6144689

Western Blot (WB) of anti-SYMPK antibody

Western Blot analysis of SYMPK expression in transfected 293T cell line by SYMPK monoclonal antibody. Lane 1: SYMPK transfected lysate (58.9kD). Lane 2: Non-transfected lysate.
Mouse Anti-SYMPK Monoclonal Antibody, Western Blot (WB) – Cat# MBS6144689

Immunohistochemistry (IHC) of anti-SYMPK antibody

Immunoperoxidase of monoclonal antibody to SYMPK on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma. [antibody concentration 3ug/ml].
Mouse Anti-SYMPK Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6144689

Immunofluorescence (IF) of anti-SYMPK antibody

Immunofluorescence of monoclonal antibody to SYMPK on HeLa cell. [antibody concentration 10ug/ml.
Mouse Anti-SYMPK Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS6144689

Other Notes

Small volumes of anti-SYMPK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SYMPK. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Related Accession #
NCBI Official Full Name
Homo sapiens symplekin, mRNA
NCBI Official Synonym Full Names
symplekin
NCBI Official Symbol
SYMPK
NCBI Official Synonym Symbols
SPK; SYM
Protein Family
NCBI Protein Information
symplekin

NCBI Summary for SYMPK

This gene encodes a nuclear protein that functions in the regulation of polyadenylation and promotes gene expression. The protein forms a high-molecular weight complex with components of the polyadenylation machinery. It is thought to serve as a scaffold for recruiting regulatory factors to the polyadenylation complex. It also participates in 3'-end maturation of histone mRNAs, which do not undergo polyadenylation. The protein also localizes to the cytoplasmic plaques of tight junctions in some cell types. [provided by RefSeq, Jul 2008]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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