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Tubulin Tyrosine Ligase (TTL) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6144975

MBS6144975 is an antibody targeting TTL. Also known as Tubulin Tyrosine Ligase (Tubulin--tyrosine Ligase, TTL) (Biotin). Reactive in Human. Host: Mouse. More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Tubulin Tyrosine Ligase (TTL), Monoclonal Antibody
Full Product Name
Tubulin Tyrosine Ligase (Tubulin--tyrosine Ligase, TTL) (Biotin)
Product Gene Name
Product Synonym Names
EC=6.3.2.25
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
1B2
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human TTL.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
43kDa
OMIM
608291
3D Structure
ModBase 3D Structure for Q8NG68
Immunogen
Partial recombinant protein corresponding to aa279-378 from human TTL (NP_714923) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
LESSILLQIKHIIRNCLLSVEPAISTKHLPYQSFQLFGFDFMVDEELKVWLIEVNGAPACAQKLYAELCQGIVDIAISSVFPPPDVEQPQTQPAAFIKL
Conjugate
Biotin

Product Categories/Family for anti-TTL antibody

Applications Tested/Suitable for anti-TTL antibody
ELISA (EIA)
Application Notes for anti-TTL antibody
ELISA: 0.1ng/ml
Applications are based on unconjugated antibody.

Testing Data of anti-TTL antibody

Detection limit for recombinant GST tagged TTL is 0.1ng/ml as a capture antibody.
Mouse Anti-TTL Monoclonal Antibody – Cat# MBS6144975

Other Notes

Small volumes of anti-TTL antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for TTL. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
tubulin--tyrosine ligase
NCBI Official Synonym Full Names
tubulin tyrosine ligase
NCBI Official Symbol
TTL
UniProt Protein Name
Tubulin--tyrosine ligase
Protein Family
UniProt Gene Name
TTL
UniProt Synonym Gene Names
TTL
UniProt Entry Name
TTL_HUMAN
NCBI Protein Information
tubulin--tyrosine ligase

NCBI Summary for TTL

TTL is a cytosolic enzyme involved in the posttranslational modification of alpha-tubulin (see MIM 602529). Alpha-tubulin within assembled microtubules is detyrosinated over time at the C terminus. After microtubule disassembly, TTL restores the tyrosine residues and consequently participates in a cycle of tubulin detyrosination and tyrosination (Erck et al., 2003 [PubMed 14571137]).[supplied by OMIM, Mar 2008]
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UniProt Comments for TTL

TTL: Catalyzes the post-translational addition of a tyrosine to the C-terminal end of detyrosinated alpha-tubulin. Belongs to the tubulin--tyrosine ligase family.

Protein type: EC 6.3.2.25; Cell development/differentiation; Ligase

Chromosomal Location of Human Ortholog: 2q13

Molecular Function: tubulin-tyrosine ligase activity; ATP binding

Biological Process: protein modification process; regulation of axon extension; microtubule cytoskeleton organization and biogenesis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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