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USP34 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6145100

MBS6145100 is an antibody targeting USP34. Also known as USP34 (Ubiquitin-specific-processing Protease 34, Ubiquitin Carboxyl-terminal Hydrolase 34, Deubiquitinating Enzyme 34, Ubiquitin Thioesterase 34, KIAA0570, KIAA0729) (Biotin). Reactive in Human. Host:… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
USP34, Monoclonal Antibody
Full Product Name
USP34 (Ubiquitin-specific-processing Protease 34, Ubiquitin Carboxyl-terminal Hydrolase 34, Deubiquitinating Enzyme 34, Ubiquitin Thioesterase 34, KIAA0570, KIAA0729) (Biotin)
Product Gene Name
Product Synonym Names
EC=3.4.19.12
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
2E2
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human USP34.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
3D Structure
ModBase 3D Structure for Q70CQ2
Immunogen
Partial recombinant corresponding to aa3296-3395 from USP34 (NP_055524) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
CKEFKDLHCSKDSTLAEEESEFPSTSISAVLSDLADLRSCDGQALPSQDPEVALSLSCGHSRGLFSHMQQHDILDTLCRTIESTIHVVTRISGKGNQAAS
Conjugate
Biotin

Product Categories/Family for anti-USP34 antibody

Applications Tested/Suitable for anti-USP34 antibody
ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-USP34 antibody
IF: 10ug/ml
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-USP34 antibody

Western Blot detection against Immunogen (36.74kD).
Mouse Anti-USP34 Monoclonal Antibody – Cat# MBS6145100

Testing Data of anti-USP34 antibody

Detection limit for recombinant GST tagged USP34 is ~1ng/ml as a capture antibody.
Mouse Anti-USP34 Monoclonal Antibody, Testing Data – Cat# MBS6145100

Immunohistochemistry (IHC) of anti-USP34 antibody

Immunoperoxidase of monoclonal antibody to USP34 on formalin-fixed paraffin-embedded human seminoma. [antibody concentration 3ug/ml]
Mouse Anti-USP34 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6145100

Immunofluorescence (IF) of anti-USP34 antibody

Immunofluorescence of monoclonal antibody to USP34 on HeLa cell. [antibody concentration 10ug/ml]
Mouse Anti-USP34 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS6145100

Product References and Citations for anti-USP34 antibody

1. Caveolin-1 (P132L), a Common Breast Cancer Mutation, Confers Mammary Cell Invasiveness and Defines a Novel Stem Cell/Metastasis-Associated Gene Signature. Bonuccelli G, Casimiro MC, Sotgia F, Wang C, Liu M, Katiyar S, Zhou J, Dew E, Capozza F, Daumer KM, Minetti C, Milliman JN, Alpy F, Rio MC, Tomasetto C, Mercier I, Flomenberg N, Frank PG, Pestell RG, Lisanti MP.Am J Pathol. 2009 May;174(5):1650-62.

Other Notes

Small volumes of anti-USP34 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for USP34. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
ubiquitin carboxyl-terminal hydrolase 34
NCBI Official Synonym Full Names
ubiquitin specific peptidase 34
NCBI Official Symbol
USP34
UniProt Protein Name
Ubiquitin carboxyl-terminal hydrolase 34
UniProt Synonym Protein Names
Deubiquitinating enzyme 34; Ubiquitin thiolesterase 34; Ubiquitin-specific-processing protease 34
UniProt Gene Name
USP34
UniProt Synonym Gene Names
KIAA0570; KIAA0729
UniProt Entry Name
UBP34_HUMAN
NCBI Protein Information
ubiquitin carboxyl-terminal hydrolase 34

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-USP34 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >1 publications with USP34 and Neoplasms
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