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ATG5 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS614519

Autophagy is an important mechanism for nonselective intracellular breakdown whereby cytosol and organelles are encapsulated in vesicles, which are then engulfed and digested by lytic vacuoles/lysosomes. ATG5 is required for autophagy. It conjugates to ATG12… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
APG5 (ATG5), Polyclonal Antibody
Full Product Name
APG5 (APG5 Autophagy 5-like, APG5-like, APG5L, Apoptosis-specific Protein, ASP, ATG5 Autophagy-related 5 Homolog, Autophagy Protein 5, hAPG5, Homolog of S Cerevisiae Autophagy 5)
Product Gene Name
anti-ATG5 antibody
Product Synonym Names
Anti -APG5 (APG5 Autophagy 5-like, APG5-like, APG5L, Apoptosis-specific Protein, ASP, ATG5 Autophagy-related 5 Homolog, Autophagy Protein 5, hAPG5, Homolog of S Cerevisiae Autophagy 5)
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a lyophilized powder. Reconstitute in 100ul of sterile water. Centrifuge to remove any insoluble material.
Purity/Purification
Serum
Serum
Specificity
IHC and WB confirmed the specificity for APG5. Species Crossreactivity: Human
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
32,447 Da
Chromosome Location
Chromosome: 6; NC_000006.11 (106632352..106773695, complement). Location: 6q21
OMIM
604261
3D Structure
ModBase 3D Structure for Q9H1Y0
Immunogen
A synthetic peptide corresponding to the C-terminal of human APG-5L. No immunogenic carrier protein was conjugated to the immunogen. Instead, Adjukine B (see the Adjuvants in biosensis' product list) has been used to orchestrate/boost the immune response.

Product Categories/Family for anti-ATG5 antibody

Antibodies; Abs to Autophagy
Applications Tested/Suitable for anti-ATG5 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-ATG5 antibody
Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry.

Immunohistochemistry (IHC) of anti-ATG5 antibody

Confocal microscopy on stained APG5 using MBS614519 in paraffin-embedded human brain section (midfrontal cortex, Alzheimer case) . APG5 appears green. Red staining is p25.
Rabbit Anti-ATG5 Polyclonal Antibody – Cat# MBS614519

Immunohistochemistry (IHC) of anti-ATG5 antibody

DAB staining of APG5 in paraffin-embedded human brain section (midfrontal cortex, Alzheimer case) using MBS614519
Rabbit Anti-ATG5 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS614519

Western Blot (WB) of anti-ATG5 antibody

Western blot on non-reduced human Neutrophils using MBS614519 at a dilution of 1:100. The detected band has an apparent molecular weight of 49 kDa corresponding to the APG5-APG12 complex molecule.
Rabbit Anti-ATG5 Polyclonal Antibody, Western Blot (WB) – Cat# MBS614519

Other Notes

Small volumes of anti-ATG5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ATG5 purchase

MBS614519 is an antibody targeting ATG5. Also known as APG5 (APG5 Autophagy 5-like, APG5-like, APG5L, Apoptosis-specific Protein, ASP, ATG5 Autophagy-related 5 Homolog, Autophagy Protein 5, hAPG5, Homolog of S Cerevisiae Autophagy 5). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ATG5. It may not necessarily be applicable to this product.

NCBI GI #
4757798
NCBI GeneID
9474
NCBI Accession #
NP_004840.1
UniProt Primary Accession #
Q9H1Y0
UniProt Secondary Accession #
O60875; Q5JVR2; Q68DI4; Q9H2B8; Q9HCZ7
UniProt Related Accession #
Q7Z3H3; Q9H1Y0
GenBank Nucleotide #
NM_004849.2
NCBI Official Full Name
autophagy protein 5
NCBI Official Synonym Full Names
ATG5 autophagy related 5 homolog (S. cerevisiae)
NCBI Official Symbol
ATG5
NCBI Official Synonym Symbols
ASP; APG5; APG5L; hAPG5; APG5-LIKE
UniProt Protein Name
Autophagy protein 5
UniProt Synonym Protein Names
APG5-like; Apoptosis-specific protein
Protein Family
Autophagy protein
UniProt Gene Name
ATG5
UniProt Synonym Gene Names
APG5L; ASP
UniProt Entry Name
ATG5_HUMAN
NCBI Protein Information
autophagy protein 5; OTTHUMP00000017823; OTTHUMP00000017824; OTTHUMP00000017825; apoptosis specific protein; apoptosis-specific protein

UniProt Comments for ATG5

ATG5: Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. The ATG5-ATG12 conjugate forms a complex with several units of ATG16. Interacts with TECPR1; the interaction is direct and does not take place when ATG16 is associated with the ATG5- ATG12 conjugate. By apoptotic stimuli. Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells. Belongs to the ATG5 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Autophagy; Ubiquitin conjugating system; Apoptosis

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: membrane; cytoplasm; pre-autophagosomal structure membrane; autophagic vacuole; axoneme

Molecular Function: protein binding; APG8 conjugating enzyme activity

Biological Process: response to drug; response to fungus; apoptosis; ventricular cardiac muscle cell development; C-terminal protein lipidation; post-translational protein modification; vasodilation; regulation of release of sequestered calcium ion into cytosol; cellular response to nitrogen starvation; heart contraction; regulation of cytokine secretion during immune response; mitochondrion degradation; innate immune response; autophagy; blood vessel remodeling; negative regulation of protein ubiquitination; negative regulation of interferon type I production; otolith development; negative regulation of apoptosis; autophagic vacuole formation
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Research Articles on ATG5

1. Observational study and meta-analysis of gene-disease association. (HuGE Navigator)

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ATG5 antibody

 Reference Product  PubMed Publications
 ATG12 antibody  >80 publications with ATG5 and ATG12
 ATG10 antibody  >19 publications with ATG5 and ATG10
 ATG16L1 antibody  >10 publications with ATG5 and ATG16L1

Pathways associated with anti-ATG5 antibody

 Products by Pathway  Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Innate Immunity Signaling Pathway antibodies  Innate Immunity Signaling Pathway Diagram
 Negative Regulators Of RIG-I/MDA5 Signaling Pathway antibodies  Negative Regulators Of RIG-I/MDA5 Signaling Pathway Diagram
 RIG-I-like Receptor Signaling Pathway antibodies  RIG-I-like Receptor Signaling Pathway Diagram
 RIG-I-like Receptor Signaling Pathway antibodies  RIG-I-like Receptor Signaling Pathway Diagram
 RIG-I/MDA5 Mediated Induction Of IFN-alpha/beta Pathways antibodies  RIG-I/MDA5 Mediated Induction Of IFN-alpha/beta Pathways Diagram
 Regulation Of Autophagy Pathway antibodies  Regulation Of Autophagy Pathway Diagram
 Regulation Of Autophagy Pathway antibodies  Regulation Of Autophagy Pathway Diagram
 Senescence And Autophagy Pathway antibodies  Senescence And Autophagy Pathway Diagram
 Shigellosis Pathway antibodies  Shigellosis Pathway Diagram

Diseases associated with anti-ATG5 antibody

 Disease Name  Pubmed Publications
 Neurodegenerative Diseases Antibodies  >22 publications with ATG5 and Neurodegenerative Diseases
 Cardiovascular Diseases Antibodies  >18 publications with ATG5 and Cardiovascular Diseases
 Melanoma Antibodies  >14 publications with ATG5 and Melanoma
 Adenocarcinoma Antibodies  >13 publications with ATG5 and Adenocarcinoma
 Leukemia Antibodies  >13 publications with ATG5 and Leukemia
 Liver Diseases Antibodies  >11 publications with ATG5 and Liver Diseases
 Carcinoma, Hepatocellular Antibodies  >11 publications with ATG5 and Carcinoma, Hepatocellular
 Inflammation Antibodies  >10 publications with ATG5 and Inflammation
 Reperfusion Injury Antibodies  >10 publications with ATG5 and Reperfusion Injury
 Liver Neoplasms Antibodies  >8 publications with ATG5 and Liver Neoplasms

Organs/Tissues associated with anti-ATG5 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >34 publications with ATG5 and Brain
 Blood Antibodies  >24 publications with ATG5 and Blood
 Liver Antibodies  >24 publications with ATG5 and Liver
 Heart Antibodies  >15 publications with ATG5 and Heart
 Muscle Antibodies  >14 publications with ATG5 and Muscle
 Kidney Antibodies  >13 publications with ATG5 and Kidney
 Embryonic Tissue Antibodies  >12 publications with ATG5 and Embryonic Tissue
 Eye Antibodies  >11 publications with ATG5 and Eye
 Lung Antibodies  >9 publications with ATG5 and Lung
 Prostate Antibodies  >7 publications with ATG5 and Prostate
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