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ZHX2 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6145225

MBS6145225 is an antibody targeting ZHX2. Also known as ZHX2 (Zinc Fingers and Homeoboxes Protein 2, Zinc Finger and Homeodomain Protein 2, Alpha-fetoprotein Regulator 1, AFP Regulator 1, AFR1, KIAA0854, Regulator of AFP, RAF) (Biotin). Reactive in Human.… More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ZHX2, Monoclonal Antibody
Full Product Name
ZHX2 (Zinc Fingers and Homeoboxes Protein 2, Zinc Finger and Homeodomain Protein 2, Alpha-fetoprotein Regulator 1, AFP Regulator 1, AFR1, KIAA0854, Regulator of AFP, RAF) (Biotin)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
5E2
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human ZHX2.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
OMIM
609185
3D Structure
ModBase 3D Structure for Q9Y6X8
Immunogen
Partial recombinant corresponding to aa691-789 from ZHX2 (NP_055758) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MEQYQHQPMADDHGYDAVARKATKPMAESPKNGGDVVPQYYKDPKKLCEEDLEKLVTRVKVGSEPAKDCLPAKPSEATSDRSEGSSRDGQGSDENEES*
Conjugate
Biotin
Applications Tested/Suitable for anti-ZHX2 antibody
ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes for anti-ZHX2 antibody
IHC-P: 3ug/ml
IF: 10ug/ml
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-ZHX2 antibody

Western Blot detection against Immunogen (36.89kD).
Mouse Anti-ZHX2 Monoclonal Antibody – Cat# MBS6145225

Western Blot (WB) of anti-ZHX2 antibody

Western Blot analysis of ZHX2 expression in transfected 293T cell line by ZHX2 monoclonal antibody Lane 1: ZHX2 transfected lysate (92.307kD). Lane 2: Non-transfected lysate.
Mouse Anti-ZHX2 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6145225

Immunohistochemistry (IHC) of anti-ZHX2 antibody

Immunoperoxidase of monoclonal antibody to ZHX2 on formalin-fixed paraffin-embedded human smooth muscle. [antibody concentration 3ug/ml]
Mouse Anti-ZHX2 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6145225

Immunofluorescence (IF) of anti-ZHX2 antibody

Immunofluorescence of monoclonal antibody to ZHX2 on HeLa cell. [antibody concentration 10ug/ml]
Mouse Anti-ZHX2 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS6145225

Western Blot (WB) of anti-ZHX2 antibody

Western blot analysis of ZHX2 over-expressed 293 cell line, cotransfected with ZHX2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ZHX2 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.
Mouse Anti-ZHX2 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6145225

Other Notes

Small volumes of anti-ZHX2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ZHX2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
Homo sapiens zinc fingers and homeoboxes 2 (ZHX2), transcript variant 2, mRNA
NCBI Official Synonym Full Names
zinc fingers and homeoboxes 2
NCBI Official Symbol
ZHX2
NCBI Official Synonym Symbols
RAF; AFR1
UniProt Protein Name
Zinc fingers and homeoboxes protein 2
UniProt Synonym Protein Names
Alpha-fetoprotein regulator 1; AFP regulator 1; Regulator of AFP; Zinc finger and homeodomain protein 2
UniProt Gene Name
ZHX2
UniProt Synonym Gene Names
AFR1; KIAA0854; RAF; AFP regulator 1
UniProt Entry Name
ZHX2_HUMAN
NCBI Protein Information
zinc fingers and homeoboxes protein 2

NCBI Summary for ZHX2

The members of the zinc fingers and homeoboxes gene family are nuclear homodimeric transcriptional repressors that interact with the A subunit of nuclear factor-Y (NF-YA) and contain two C2H2-type zinc fingers and five homeobox DNA-binding domains. This gene encodes member 2 of this gene family. In addition to forming homodimers, this protein heterodimerizes with member 1 of the zinc fingers and homeoboxes family. [provided by RefSeq, Jul 2008]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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