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ASB9 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6146001

MBS6146001 is an antibody targeting ASB9. Also known as ASB9 (Ankyrin Repeat and SOCS Box Protein 9, ASB-9, DKFZp564L0862, FLJ20636, MGC4954) (FITC). Reactive in Human. Host: Mouse. More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ASB9, Monoclonal Antibody
Full Product Name
ASB9 (Ankyrin Repeat and SOCS Box Protein 9, ASB-9, DKFZp564L0862, FLJ20636, MGC4954) (FITC)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
1D8
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human ASB9.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
26,849 Da
Immunogen
Full length recombinant protein corresponding to aa1-253 from human ASB9 (AAH01244) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MDGKQGGMDGSKPAGPRDFPGIRLLSNPLMGDAVSDWSPMHEAAIHGHQLSLRNLISQGWAVNIITADHVSPLHEACLGGHLSCVKILLKHGAQVNGVTADWHTPLFNACVSGSWDCVNLLLQHGASVQPESDLASPIHEAARRAYGGNIDHKISHLGTPLYLACENQQRACVKKLLESGADVNQGKGQDSPLHAVARTASEELACLLMDFGADTQAKNAEGKRPVELVPPESPLAQLFLEREGASLPKPKP
Conjugate
FITC

Product Categories/Family for anti-ASB9 antibody

Applications Tested/Suitable for anti-ASB9 antibody
ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Immunoprecipitation (IP), Western Blot (WB)
Application Notes for anti-ASB9 antibody
IHC-P: 3ug/ml
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-ASB9 antibody

Western Blot detection against Immunogen (53.83kD).
Mouse Anti-ASB9 Monoclonal Antibody – Cat# MBS6146001

Western Blot (WB) of anti-ASB9 antibody

Western Blot analysis of ASB9 expression in transfected 293T cell line by ASB9 monoclonal antibody. Lane 1: ASB9 transfected lysate (31.9kD). Lane 2: Non-transfected lysate.
Mouse Anti-ASB9 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6146001

Immunohistochemistry (IHC) of anti-ASB9 antibody

Immunoperoxidase of monoclonal antibody to ASB9 on formalin-fixed paraffin-embedded human lymphoma tissue. [antibody concentration 3ug/ml]
Mouse Anti-ASB9 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6146001

Immunoprecipitation (IP) of anti-ASB9 antibody

Immunoprecipitation of ASB9 transfected lysate using ASB9 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with ASB9 rabbit polyclonal antibody.
Mouse Anti-ASB9 Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS6146001

Testing Data of anti-ASB9 antibody

Detection limit for recombinant GST tagged ASB9 is ~0.1ng/ml as a capture antibody.
Mouse Anti-ASB9 Monoclonal Antibody, Testing Data – Cat# MBS6146001

Western Blot (WB) of anti-ASB9 antibody

ASB9 monoclonal antibody, Western Blot analysis of ASB9 expression in HepG2.
Mouse Anti-ASB9 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6146001

Other Notes

Small volumes of anti-ASB9 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ASB9. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Homo sapiens ankyrin repeat and SOCS box-containing 9, mRNA
NCBI Official Synonym Full Names
ankyrin repeat and SOCS box containing 9
NCBI Official Symbol
ASB9
NCBI Protein Information
ankyrin repeat and SOCS box protein 9

NCBI Summary for ASB9

This gene encodes a member of the ankyrin repeat and suppressor of cytokine signaling (SOCS) box protein family. Members of this family can interact with the elongin B-C adapter complex via their SOCS box domain and further complex with the cullin and ring box proteins to form E3 ubiquitin ligase complexes. They may function to mediate the substrate-recognition of the E3 ubiquitin ligases. A transcribed pseudogene of this gene has been identified on chromosome 15. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2009]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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