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MAP Kinase, P44/42 (MAPK1) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS614655

Both p44 and p42 MAP kinases (Erk1 and Erk2) function in a protein kinase cascade that plays a critical role in the regulation of cell growth and differentiation. MAP kinases are activated by a wide variety of extracellular signals including growth and… More details

$750 / 0.2 mL +1 FREE 8GB USB
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
MAP Kinase, p44/42 (MAPK1), Polyclonal Antibody
Full Product Name
MAP Kinase, p44/42 (Erk1/Erk2, Extracellular Signal Regulated Kinase)
Product Gene Name
anti-MAPK1 antibody
Product Synonym Names
Anti -MAP Kinase, p44/42 (Erk1/Erk2, Extracellular Signal Regulated Kinase)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Hamster, Human, Mouse, Rat
Form/Format
Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Purity/Purification
Affinity Purified
Purified by Protein A and immunoaffinity chromatography.
Specificity
Recognizes endogenous levels of total human p44/42 MAP kinase (Erk1/Erk2) protein. In some cell types, recognizes p44 MAPK more readily than p42 MAPK. Does not recognize JNK/SAPK nor p38 MAP kinase. Species Crossreactivity: Human, mouse, rat, hamster, zebrafish.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Chromosome Location
Chromosome: 15; NC_006102.3 (510690..521361). Location: chromosome: 15
Immunogen
Synthetic peptide derived from the sequence in the C-terminus of rat p42 MAP kinase, coupled to KLH.

Product Categories/Family for anti-MAPK1 antibody

Antibodies; Abs to Protein Kinases
Applications Tested/Suitable for anti-MAPK1 antibody
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Application Notes for anti-MAPK1 antibody
Suitable for use in Immunofluorescence, Immunocytochemistry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Flow Cytometry.
Dilution: Western Blot: 1:1000; Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight.
Immunohistochemistry(Paraffin sections): 1:100
Immunoprecipitation: 1:50
Immunocytochemistry (IF): 1:100
Flow Cytometry: 1:50

Western Blot (WB) of anti-MAPK1 antibody

Western Blot analysis of extracts from serum-induced PC12 cells, using MBS614655A (upper) or control (lower).
Rabbit Anti-MAPK1 Polyclonal Antibody – Cat# MBS614655

Immunohistochemistry (IHC) of anti-MAPK1 antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing nuclear and cytoplasmic localization, using MBS614655
Rabbit Anti-MAPK1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS614655

Flow Cytometry (FC/FACS) of anti-MAPK1 antibody

Flow cytometric analysis of Jurkat cells, using MBS614655 (blue) compared to a nonspecific negative control antibody (red).
Rabbit Anti-MAPK1 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS614655

Other Notes

Small volumes of anti-MAPK1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MAPK1 purchase

MBS614655 is an antibody targeting MAPK1. Also known as MAP Kinase, p44/42 (Erk1/Erk2, Extracellular Signal Regulated Kinase). Reactive in Hamster, Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MAPK1. It may not necessarily be applicable to this product.

NCBI GI #
45383812
NCBI GeneID
373953
NCBI Accession #
NP_989481.1
GenBank Nucleotide #
NM_204150.1
NCBI Official Full Name
mitogen-activated protein kinase 1
NCBI Official Synonym Full Names
mitogen-activated protein kinase 1
NCBI Official Symbol
MAPK1
NCBI Protein Information
mitogen-activated protein kinase 1

Research Articles on MAPK1

1. TRPC3 functions as both a Ca(2+)-permeable channel and a protein scaffold at the plasma membrane for downstream PKCbeta activation in B cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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