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CLTB Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6146561

MBS6146561 is an antibody targeting CLTB. Also known as CLTB (Clathrin Light Chain B, Lcb) (FITC). Reactive in Human. Host: Mouse. More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CLTB, Monoclonal Antibody
Full Product Name
CLTB (Clathrin Light Chain B, Lcb) (FITC)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
4B12-1E3
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human CLTB.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Full length recombinant corresponding to aa1-211 from human CLTB (AAH06457) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MADDFGFFSSSESGAPEAAEEDPAAAFLAQQESEIAGIENDEGFGAPAGSHAAPAQPGPTSGAGSEDMGTTVNGDVFQEANGPADGYAAIAQADRLTQEPESIRKWREEQRKRLQELDAASKVTEQEWREKAKKDLEEWNQRQSEQVEKNKINNRASEEAFVKESKEETPGTEWEKVAQLCDFNPKSSKQCKDVSRLRSVLMSLKQTPLSR
Conjugate
FITC

Product Categories/Family for anti-CLTB antibody

Applications Tested/Suitable for anti-CLTB antibody
ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-CLTB antibody
IF: 10ug/ml
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-CLTB antibody

Western Blot detection against Immunogen (48.95kD).
Mouse Anti-CLTB Monoclonal Antibody – Cat# MBS6146561

Western Blot (WB) of anti-CLTB antibody

CLTB monoclonal antibody, Western Blot analysis of CLTB expression in MCF-7.
Mouse Anti-CLTB Monoclonal Antibody, Western Blot (WB) – Cat# MBS6146561

Western Blot (WB) of anti-CLTB antibody

Western Blot analysis of CLTB expression in transfected 293T cell line by CLTB monoclonal antibody. Lane 1: CLTB transfected lysate (23.2kD). Lane 2: Non-transfected lysate.
Mouse Anti-CLTB Monoclonal Antibody, Western Blot (WB) – Cat# MBS6146561

Immunohistochemistry (IHC) of anti-CLTB antibody

Immunoperoxidase of monoclonal antibody to CLTB on formalin-fixed paraffin-embedded human transitional cell carcinoma tissue. [antibody concentration 2ug/ml].
Mouse Anti-CLTB Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6146561

Immunofluorescence (IF) of anti-CLTB antibody

Immunofluorescence of monoclonal antibody to CLTB on HeLa cell. [antibody concentration 10ug/ml].
Mouse Anti-CLTB Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS6146561

Other Notes

Small volumes of anti-CLTB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CLTB. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Related Accession #
NCBI Official Full Name
Homo sapiens clathrin, light chain (Lcb), mRNA
NCBI Official Synonym Full Names
clathrin light chain B
NCBI Official Symbol
CLTB
NCBI Official Synonym Symbols
LCB
Protein Family
NCBI Protein Information
clathrin light chain B

NCBI Summary for CLTB

Clathrin is a large, soluble protein composed of heavy and light chains. It functions as the main structural component of the lattice-type cytoplasmic face of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. This gene encodes one of two clathrin light chain proteins which are believed to function as regulatory elements. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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