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DOCK4 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6146897

MBS6146897 is an antibody targeting DOCK4. Also known as DOCK4 (Dedicator of Cytokinesis Protein 4, FLJ34238, KIAA0716, MGC134911, MGC134912) (FITC). Reactive in Human. Host: Mouse. More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
DOCK4, Monoclonal Antibody
Full Product Name
DOCK4 (Dedicator of Cytokinesis Protein 4, FLJ34238, KIAA0716, MGC134911, MGC134912) (FITC)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
3E7
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human DOCK4.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
OMIM
AY233380 mRNA
3D Structure
ModBase 3D Structure for Q8N1I0
Immunogen
Partial recombinant corresponding to aa1867-1966 from DOCK4 (NP_055520) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
NQVNEQSAPLPVPVPVPVPSYGGEEPVRKESKTPPPYSVYERTLRRPVPLPHSLSIPVTSEPPALPPKPLAARSSHLENGARRTDPGPRPRPLPRKVSQL
Conjugate
FITC

Product Categories/Family for anti-DOCK4 antibody

Applications Tested/Suitable for anti-DOCK4 antibody
ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-DOCK4 antibody
IF: 10ug/ml
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-DOCK4 antibody

Western Blot detection against Immunogen (36.74kD).
Mouse Anti-DOCK4 Monoclonal Antibody – Cat# MBS6146897

Western Blot (WB) of anti-DOCK4 antibody

DOCK4 monoclonal antibody. Western Blot analysis of DOCK4 expression in HeLa.
Mouse Anti-DOCK4 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6146897

Immunohistochemistry (IHC) of anti-DOCK4 antibody

Immunoperoxidase of monoclonal antibody to DOCK4 on formalin-fixed paraffin-embedded human breast cancer. [antibody concentration 3ug/ml]
Mouse Anti-DOCK4 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6146897

Immunofluorescence (IF) of anti-DOCK4 antibody

Immunofluorescence of monoclonal antibody to DOCK4 on HeLa cell. [antibody concentration 10ug/ml.
Mouse Anti-DOCK4 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS6146897

Testing Data of anti-DOCK4 antibody

Detection limit for recombinant GST tagged DOCK4 is ~0.1ng/ml as a capture antibody.
Mouse Anti-DOCK4 Monoclonal Antibody, Testing Data – Cat# MBS6146897

Product References and Citations for anti-DOCK4 antibody

1. Identification of novel posttranscriptional targets of the BCR/ABL oncoprotein by ribonomics: requirement of E2F3 for BCR/ABL leukemogenesis.Eiring AM, Neviani P, Santhanam R, Oaks JJ, Chang JS, Notari M, Willis W, Gambacorti-Passerini C, Volinia S, Marcucci G, Caligiuri MA, Leone GW, Perrotti D.Blood. 2008 Jan 15;111(2):816-28. Epub 2007 Oct 9.

Other Notes

Small volumes of anti-DOCK4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for DOCK4. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
dedicator of cytokinesis protein 4 isoform 1
NCBI Official Synonym Full Names
dedicator of cytokinesis 4
NCBI Official Symbol
DOCK4
UniProt Protein Name
Dedicator of cytokinesis protein 4
UniProt Gene Name
DOCK4
UniProt Synonym Gene Names
KIAA0716
NCBI Protein Information
dedicator of cytokinesis protein 4

NCBI Summary for DOCK4

This gene is a member of the dedicator of cytokinesis (DOCK) family and encodes a protein with a DHR-1 (CZH-1) domain, a DHR-2 (CZH-2) domain and an SH3 domain. This membrane-associated, cytoplasmic protein functions as a guanine nucleotide exchange factor and is involved in regulation of adherens junctions between cells. Mutations in this gene have been associated with ovarian, prostate, glioma, and colorectal cancers. Alternatively spliced variants which encode different protein isoforms have been described, but only one has been fully characterized. [provided by RefSeq, Jul 2008]
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UniProt Comments for DOCK4

Involved in regulation of adherens junction between cells. Plays a role in cell migration. Functions as a guanine nucleotide exchange factor (GEF), which activates Rap1 small GTPase by exchanging bound GDP for free GTP.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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