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Glycerol-3-Phosphate Dehydrogenase (GPDH) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6147401

GRB14, GPD1, and GDF8 as potential network collaborators in weight loss-induced improvements in insulin action in human skeletal muscle. More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Glycerol-3-Phosphate Dehydrogenase (GPDH), Monoclonal Antibody
Full Product Name
Glycerol-3-Phosphate Dehydrogenase (GPDH, GPD1, GPD-C, GPDH-C, FLJ26652) (FITC)
Product Gene Name
anti-GPDH antibody
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
3C10-1C4
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human GPD1.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
35,140 Da
Immunogen
Full length recombinant corresponding to aa1-349 from human GPD1 (AAH32234) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MASKKVCIVGSGNWGSAIAKIVGGNAAQLAQFDPRVTMWVFEEDIGGKKLTEIINTQHENVKYLPGHKLPPNVVAVPDVVQAAEDADILIFVVPHQFIGKICDQLKGHLKANATGISLIKGVDEGPNGLKLISEVIGERLGIPMSVLMGANIASEVADEKFCETTIGCKDPAQGQLLKELMQTPNFRITVVQEVDTVEICGTLKNVVAVGAGFCDGLGFGDNTKAAVIRLGLMEMIAFAKLFCSGPVSSATFLESCGVADLITTCYGGRNRKVAEAFARTGKSIEQLEKELLNGQKLQGPETARELYSILQHKGLVDKFPLFMAVYKVCYEGQPVGEFIHCLQNHPEHM
Conjugate
FITC

Product Categories/Family for anti-GPDH antibody

Antibodies; Enzymes; Dehydrogenase
Applications Tested/Suitable for anti-GPDH antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-GPDH antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-GPDH antibody

Western Blot detection against Immunogen (64.13kD).
Mouse Anti-GPDH Monoclonal Antibody – Cat# MBS6147401

Western Blot (WB) of anti-GPDH antibody

GPD1 monoclonal antibody. Western Blot analysis of GPD1 expression in human kidney.
Mouse Anti-GPDH Monoclonal Antibody, Western Blot (WB) – Cat# MBS6147401

Testing Data of anti-GPDH antibody

Detection limit for recombinant GST tagged GPD1 is ~1ng/ml as a capture antibody.
Mouse Anti-GPDH Monoclonal Antibody, Testing Data – Cat# MBS6147401

Other Notes

Small volumes of anti-GPDH antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GPDH purchase

MBS6147401 is an antibody targeting GPDH. Also known as Glycerol-3-Phosphate Dehydrogenase (GPDH, GPD1, GPD-C, GPDH-C, FLJ26652) (FITC). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for GPDH. It may not necessarily be applicable to this product.

NCBI GI #
21594876
NCBI GeneID
2819
NCBI Accession #
BC032234
NCBI Related Accession #
AAH32234
UniProt Secondary Accession #
Q8N1B0; F8W1L5
UniProt Related Accession #
P21695
NCBI Official Full Name
Homo sapiens glycerol-3-phosphate dehydrogenase 1 (soluble), mRNA
NCBI Official Synonym Full Names
glycerol-3-phosphate dehydrogenase 1
NCBI Official Symbol
GPD1
NCBI Official Synonym Symbols
GPD-C; HTGTI; GPDH-C
Protein Family
Glycerol-3-phosphate dehydrogenase
NCBI Protein Information
glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic

NCBI Summary for GPDH

This gene encodes a member of the NAD-dependent glycerol-3-phosphate dehydrogenase family. The encoded protein plays a critical role in carbohydrate and lipid metabolism by catalyzing the reversible conversion of dihydroxyacetone phosphate (DHAP) and reduced nicotine adenine dinucleotide (NADH) to glycerol-3-phosphate (G3P) and NAD+. The encoded cytosolic protein and mitochondrial glycerol-3-phosphate dehydrogenase also form a glycerol phosphate shuttle that facilitates the transfer of reducing equivalents from the cytosol to mitochondria. Mutations in this gene are a cause of transient infantile hypertriglyceridemia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012]
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Research Articles on GPDH

1. The R269A mutation of GPDH1 results in a 110-fold increase in K(m) (2.8 kcal/mol effect) and a 41,000-fold decrease in k(cat) (6.3 kcal/mol effect), which corresponds to a 9.1 kcal/mol destabilization of the transition state.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-GPDH antibody

 Products by Pathway  Pathway Diagram
 Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway antibodies  Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway Diagram
 Glycerophospholipid Biosynthesis Pathway antibodies  Glycerophospholipid Biosynthesis Pathway Diagram
 Glycerophospholipid Metabolism Pathway antibodies  Glycerophospholipid Metabolism Pathway Diagram
 Glycerophospholipid Metabolism Pathway antibodies  Glycerophospholipid Metabolism Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 Phospholipid Metabolism Pathway antibodies  Phospholipid Metabolism Pathway Diagram
 Synthesis Of PA Pathway antibodies  Synthesis Of PA Pathway Diagram
 Triacylglyceride Synthesis Pathway antibodies  Triacylglyceride Synthesis Pathway Diagram
 Triglyceride Biosynthesis Pathway antibodies  Triglyceride Biosynthesis Pathway Diagram

Diseases associated with anti-GPDH antibody

 Disease Name  Pubmed Publications
 Heart Diseases Antibodies  >8 publications with GPDH and Heart Diseases
 Cardiovascular Diseases Antibodies  >8 publications with GPDH and Cardiovascular Diseases
 Neoplasms Antibodies  >5 publications with GPDH and Neoplasms
 Nervous System Diseases Antibodies  >3 publications with GPDH and Nervous System Diseases
 Inflammation Antibodies  >3 publications with GPDH and Inflammation
 Diabetes Mellitus Antibodies  >2 publications with GPDH and Diabetes Mellitus
 Liver Diseases Antibodies  >2 publications with GPDH and Liver Diseases
 Carcinoma, Hepatocellular Antibodies  >2 publications with GPDH and Carcinoma, Hepatocellular
 Weight Loss Antibodies  >1 publications with GPDH and Weight Loss
 Hyperglycemia Antibodies  >1 publications with GPDH and Hyperglycemia

Organs/Tissues associated with anti-GPDH antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >8 publications with GPDH and Muscle
 Heart Antibodies  >7 publications with GPDH and Heart
 Kidney Antibodies  >6 publications with GPDH and Kidney
 Adipose Tissue Antibodies  >5 publications with GPDH and Adipose Tissue
 Liver Antibodies  >4 publications with GPDH and Liver
 Blood Antibodies  >4 publications with GPDH and Blood
 Brain Antibodies  >3 publications with GPDH and Brain
 Testis Antibodies  >2 publications with GPDH and Testis
 Embryonic Tissue Antibodies  >2 publications with GPDH and Embryonic Tissue
 Connective Tissue Antibodies  >2 publications with GPDH and Connective Tissue
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