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GMEB1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6147407

MBS6147407 is an antibody targeting GMEB1. Also known as GMEB1 (Glucocorticoid Modulatory Element-binding Protein 1, GMEB-1, DNA-binding Protein p96PIF, Parvovirus Initiation Factor p96, PIF p96) (FITC). Reactive in Human. Host: Mouse. More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
GMEB1, Monoclonal Antibody
Full Product Name
GMEB1 (Glucocorticoid Modulatory Element-binding Protein 1, GMEB-1, DNA-binding Protein p96PIF, Parvovirus Initiation Factor p96, PIF p96) (FITC)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
2A8
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human GMEB1.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
61kDa
3D Structure
ModBase 3D Structure for B1AT47
Immunogen
Partial recombinant corresponding to aa467-563 from human GMEB1 (NP_077808) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
TAMQDGSTLGNMTTMVSPVELVAMESGLTSAIQAVESTSEDGQTIIEIDPAPDPEAEDTEGKAVILETELRTEEKVVAEMEEHQHQVHNVEIVVLED
Conjugate
FITC

Product Categories/Family for anti-GMEB1 antibody

Applications Tested/Suitable for anti-GMEB1 antibody
ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-GMEB1 antibody
IF: 10ug/ml
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-GMEB1 antibody

Western Blot detection against Immunogen (36.78kD).
Mouse Anti-GMEB1 Monoclonal Antibody – Cat# MBS6147407

Western Blot (WB) of anti-GMEB1 antibody

GMEB1 monoclonal antibody Western Blot analysis of GMEB1 expression in HeLa NE.
Mouse Anti-GMEB1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6147407

Immunohistochemistry (IHC) of anti-GMEB1 antibody

Immunoperoxidase of monoclonal antibody to GMEB1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3ug/ml].
Mouse Anti-GMEB1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6147407

Immunofluorescence (IF) of anti-GMEB1 antibody

Immunofluorescence of monoclonal antibody to GMEB1 on HeLa cell. [antibody concentration 10ug/ml].
Mouse Anti-GMEB1 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS6147407

Testing Data of anti-GMEB1 antibody

Detection limit for recombinant GST tagged GMEB1 is ~0.03ng/ml as a capture antibody.
Mouse Anti-GMEB1 Monoclonal Antibody, Testing Data – Cat# MBS6147407

Other Notes

Small volumes of anti-GMEB1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for GMEB1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
glucocorticoid modulatory element-binding protein 1 isoform 2
NCBI Official Synonym Full Names
glucocorticoid modulatory element binding protein 1
NCBI Official Symbol
GMEB1
NCBI Official Synonym Symbols
PIF96; P96PIF
UniProt Protein Name
Glucocorticoid modulatory element binding protein 1, isoform CRA_a
UniProt Synonym Protein Names
Glucocorticoid modulatory element binding protein 1, isoform CRA_a
UniProt Gene Name
GMEB1
UniProt Entry Name
B1AT47_HUMAN
NCBI Protein Information
glucocorticoid modulatory element-binding protein 1

NCBI Summary for GMEB1

This gene encodes a member of KDWK gene family which associates with GMEB2 protein. The GMEB1-GMEB2 complex is essential for parvovirus DNA replication. Studies in rat for a similar gene suggest that this gene's role is to modulate the transactivation of the glucocorticoid receptor when it is bound to glucocorticoid response elements. Three alternative spliced transcript variants encoding different isoforms exist. [provided by RefSeq, Feb 2016]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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