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GOLM1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6147438

MBS6147438 is an antibody targeting GOLM1. Also known as GOLM1 (Golgi Membrane Protein 1, Golgi Membrane Protein GP73, Golgi Phosphoprotein 2, C9orf155, GOLPH2, PSEC0242, UNQ686/PRO1326, FLJ22634, FLJ23608) (FITC). Reactive in Human. Host: Mouse. More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
GOLM1, Monoclonal Antibody
Full Product Name
GOLM1 (Golgi Membrane Protein 1, Golgi Membrane Protein GP73, Golgi Phosphoprotein 2, C9orf155, GOLPH2, PSEC0242, UNQ686/PRO1326, FLJ22634, FLJ23608) (FITC)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
5B10
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human GOLPH2.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
45,333 Da
3D Structure
ModBase 3D Structure for B3KNK9
Immunogen
Partial recombinant corresponding to aa302-402 from human GOLPH2 (NP_057632) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
VQAALSVSQENPEMEGPERDQLVIPDGQEEEQEAAGEGRNQQKLRGEDDYNMDENEAESETDKQAALAGNDRNIDVFNVEDQKRDTINLLDQREKRNHTL
Conjugate
FITC

Product Categories/Family for anti-GOLM1 antibody

Applications Tested/Suitable for anti-GOLM1 antibody
ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-GOLM1 antibody
IF: 10ug/ml
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-GOLM1 antibody

Western Blot detection against Immunogen (37.11kD).
Mouse Anti-GOLM1 Monoclonal Antibody – Cat# MBS6147438

Western Blot (WB) of anti-GOLM1 antibody

Western Blot analysis of GOLM1 expression in transfected 293T cell line by GOLPH2 monoclonal antibody. Lane 1: GOLM1 transfected lysate (45.3kD). Lane 2: Non-transfected lysate.
Mouse Anti-GOLM1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6147438

Immunohistochemistry (IHC) of anti-GOLM1 antibody

Immunoperoxidase of monoclonal antibody to GOLPH2 on formalin-fixed paraffin-embedded human endometrium. [antibody concentration 3ug/ml].
Mouse Anti-GOLM1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6147438

Immunofluorescence (IF) of anti-GOLM1 antibody

Immunofluorescence of monoclonal antibody to GOLM1 on HeLa cell. [antibody concentration 10ug/ml].
Mouse Anti-GOLM1 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS6147438

Testing Data of anti-GOLM1 antibody

Detection limit for recombinant GST tagged GOLPH2 is ~0.1ng/ml as a capture antibody.
Mouse Anti-GOLM1 Monoclonal Antibody, Testing Data – Cat# MBS6147438

Other Notes

Small volumes of anti-GOLM1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for GOLM1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
Golgi membrane protein 1
NCBI Official Synonym Full Names
golgi membrane protein 1
NCBI Official Symbol
GOLM1
NCBI Official Synonym Symbols
GP73; HEL46; GOLPH2; C9orf155; PSEC0257; bA379P1.3
UniProt Protein Name
Epididymis luminal protein 46
UniProt Synonym Protein Names
Epididymis luminal protein 46
UniProt Gene Name
GOLPH2
UniProt Entry Name
B3KNK9_HUMAN
NCBI Protein Information
Golgi membrane protein 1; epididymis luminal protein 46; golgi membrane protein GP73; golgi phosphoprotein 2; golgi protein, 73-kD

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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