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Hemopexin (HPX) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6147557

Hemopexin is a serum glycoprotein that binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation. Structurally hemopexin consists of two similar halves of approximately 200aa residues… More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Hemopexin (HPX), Monoclonal Antibody
Full Product Name
Hemopexin (HPX, HX, Beta-1B-glycoprotein, FLJ56652, Hemo) (FITC)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
3A9-1A9
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human HPX.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
51,676 Da
Immunogen
Full length recombinant corresponding to aa1-254 from human HPX (AAH05395) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MARVLGAPVALGLWSLCWSLAIATPLPPTSAHGNVAEGETKADPDVTERCSDGWSFDATTLDDNGTMLFFKGEFVWKSHKWDRELISERWKNFPSPVDAAFRQGHNSVFLIKGDKVWVYPPEKKEKGYPKLLQDEFPGIPSPLDAAVECHRGECQAEGVLFFQGDREWFWDLATGTMKERSWPAVGNCSSALRWLGRYYCFQGNQFLRFDPVRGEVPPRYPRDVRDYFMPCPGRGHGHRNGTGHGNSICAVAHI
Conjugate
FITC

Product Categories/Family for anti-HPX antibody

Applications Tested/Suitable for anti-HPX antibody
ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB)
Application Notes for anti-HPX antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-HPX antibody

Western Blot detection against Immunogen (53.68kD).
Mouse Anti-HPX Monoclonal Antibody – Cat# MBS6147557

Western Blot (WB) of anti-HPX antibody

HPX monoclonal antibody Western Blot analysis of HPX expression in human liver.
Mouse Anti-HPX Monoclonal Antibody, Western Blot (WB) – Cat# MBS6147557

Western Blot (WB) of anti-HPX antibody

Western Blot analysis of HPX expression in transfected 293T cell line by HPX monoclonal antibody. Lane 1: HPX transfected lysate (Predicted MW: 28.6kD). Lane 2: Non-transfected lysate
Mouse Anti-HPX Monoclonal Antibody, Western Blot (WB) – Cat# MBS6147557

Immunoprecipitation (IP) of anti-HPX antibody

Immunoprecipitation of HPX transfected lysate using HPX monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with HPX rabbit polyclonal antibody.
Mouse Anti-HPX Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS6147557

Western Blot (WB) of anti-HPX antibody

Western blot analysis of HPX over-expressed 293 cell line, cotransfected with HPX Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with HPX monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.
Mouse Anti-HPX Monoclonal Antibody, Western Blot (WB) – Cat# MBS6147557

Other Notes

Small volumes of anti-HPX antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HPX purchase

MBS6147557 is an antibody targeting HPX. Also known as Hemopexin (HPX, HX, Beta-1B-glycoprotein, FLJ56652, Hemo) (FITC). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for HPX. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Homo sapiens hemopexin, mRNA
NCBI Official Synonym Full Names
hemopexin
NCBI Official Symbol
HPX
NCBI Official Synonym Symbols
HX
Protein Family
NCBI Protein Information
hemopexin

NCBI Summary for HPX

This gene encodes a plasma glycoprotein that binds heme with high affinity. The encoded protein is an acute phase protein that transports heme from the plasma to the liver and may be involved in protecting cells from oxidative stress. [provided by RefSeq, Apr 2009]

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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