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HLA-DQA1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6147598

MBS6147598 is an antibody targeting HLA-DQA1. Also known as HLA-DQA1 (HLA Class II Histocompatibility Antigen, DQ alpha 1 Chain, DC-1 alpha Chain, DC-alpha, HLA-DCA, MHC Class II DQA1) (FITC). Reactive in Human. Host: Mouse. More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
HLA-DQA1, Monoclonal Antibody
Full Product Name
HLA-DQA1 (HLA Class II Histocompatibility Antigen, DQ alpha 1 Chain, DC-1 alpha Chain, DC-alpha, HLA-DCA, MHC Class II DQA1) (FITC)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
1A3
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human HLA-DQA1.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
254
Chromosome Location
Chromosome: 6; NC_000006.11 (32605169..32612152). Location: 6p21.3
OMIM
146880
3D Structure
ModBase 3D Structure for P01909
Immunogen
Partial recombinant corresponding to aa24-110 from human HLA-DQA1 (NP_002113.2) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
EDIVADHVASCGVNLYQFYGPSGQYTHEFDGDEEFYVDLERKETAWRWPEFSKFGGFDPQGALRNMAVAKHNLNIMIKRYNSTAATN
Conjugate
FITC

Product Categories/Family for anti-HLA-DQA1 antibody

Applications Tested/Suitable for anti-HLA-DQA1 antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-HLA-DQA1 antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-HLA-DQA1 antibody

Western Blot detection against Immunogen (35.31kD).
Mouse Anti-HLA-DQA1 Monoclonal Antibody – Cat# MBS6147598

Testing Data of anti-HLA-DQA1 antibody

Detection limit for recombinant GST tagged HLA-DQA1 is 0.3ng/ml as a capture antibody.
Mouse Anti-HLA-DQA1 Monoclonal Antibody, Testing Data – Cat# MBS6147598

Testing Data of anti-HLA-DQA1 antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between CD74 and HLA-DQA1. HeLa cells were stained with CD74 rabbit purified polyclonal 1:1200 and HLA-DQA1 mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
Mouse Anti-HLA-DQA1 Monoclonal Antibody, Testing Data – Cat# MBS6147598

Other Notes

Small volumes of anti-HLA-DQA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for HLA-DQA1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
HLA class II histocompatibility antigen, DQ alpha 1 chain
NCBI Official Synonym Full Names
major histocompatibility complex, class II, DQ alpha 1
NCBI Official Symbol
HLA-DQA1
NCBI Official Synonym Symbols
CD; GSE; DQ-A1; CELIAC1; HLA-DQA
UniProt Protein Name
HLA class II histocompatibility antigen, DQ alpha 1 chain
UniProt Synonym Protein Names
DC-1 alpha chain; DC-alpha; HLA-DCA; MHC class II DQA1
UniProt Gene Name
HLA-DQA1
UniProt Entry Name
DQA1_HUMAN
NCBI Protein Information
HLA class II histocompatibility antigen, DQ alpha 1 chain; HLA-DCA; DC-alpha; DC-1 alpha chain; MHC HLA-DQ alpha; MHC class II DQA1; MHC class II antigen; leucocyte antigen DQA1; MHC class II HLA-DQ-alpha-1; leukocyte antigen alpha chain; MHC class II sur

UniProt Comments for HLA-DQA1

HLA-DQA1 iso4:

Protein type: Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: Golgi membrane; membrane; lysosomal membrane; integral to plasma membrane; plasma membrane; trans-Golgi network membrane; endosome membrane; MHC class II protein complex; external side of plasma membrane

Molecular Function: MHC class II receptor activity; peptide antigen binding

Biological Process: positive regulation of T cell differentiation; cytokine and chemokine mediated signaling pathway; T cell costimulation; antigen processing and presentation of exogenous peptide antigen via MHC class II; immune response; T cell receptor signaling pathway
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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