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Phosphatase (CDC14B) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS614763

Cdc14, also known as duel specificity phosphatase, is a protein phosphatase originally discovered in yeast that plays an important role in cell cycle regulation, including exit from mitosis (1). Throughout G1, S and G2 phases, Cdc14 is located in the… More details

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Product Documentation
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
cdc14B Phosphatase, Polyclonal Antibody
Full Product Name
cdc14B Phosphatase
Product Gene Name
anti-CDC14B antibody
Product Synonym Names
Anti -cdc14B Phosphatase
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Specificity
Recognizes the human Cdc14B protein including isoforms 1, 2 and 3. The calculated molecular weight of Cdc14B isoforms range from 53-60kD. The apparent molecular weight of Cdc14B in HeLa cells was ~66kD. Reactivity is confirmed with human HeLa cell lysates.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
56,802 Da
Chromosome Location
Chromosome: 9; NC_000009.11 (99262395..99382112, complement). Location: 9q22.3
OMIM
603505
3D Structure
ModBase 3D Structure for O60729
Immunogen
Synthetic peptide derived from the N-terminal region of the human Cdc14B protein
Positive Control
Human HeLa cell lysates

Product Categories/Family for anti-CDC14B antibody

Antibodies; Abs to Protein Kinases
Applications Tested/Suitable for anti-CDC14B antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-CDC14B antibody
Suitable for use in Immunoprecipitation and Western Blot.
Dilution: Immunoprecipitation: 1-3ug/ml
Western Blot: 1-3ug/ml

Other Notes

Small volumes of anti-CDC14B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CDC14B purchase

MBS614763 is an antibody targeting CDC14B. Also known as cdc14B Phosphatase. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CDC14B. It may not necessarily be applicable to this product.

NCBI GI #
5706724
NCBI GeneID
8555
UniProt Primary Accession #
O60729
UniProt Secondary Accession #
O43183; O60730; Q5JU08; A6N5X8; B1AL31; B1AL32
UniProt Related Accession #
O60729; Q5JU06
NCBI Official Full Name
Cdc14B3 phosphatase
NCBI Official Synonym Full Names
CDC14 cell division cycle 14 homolog B (S. cerevisiae)
NCBI Official Symbol
CDC14B
NCBI Official Synonym Symbols
CDC14B3; Cdc14B1; Cdc14B2; hCDC14B
UniProt Protein Name
Dual specificity protein phosphatase CDC14B
UniProt Synonym Protein Names
CDC14 cell division cycle 14 homolog B
Protein Family
Dual specificity protein phosphatase
UniProt Gene Name
CDC14B
UniProt Entry Name
CC14B_HUMAN
NCBI Protein Information
dual specificity protein phosphatase CDC14B; OTTHUMP00000021729; OTTHUMP00000021730; OTTHUMP00000021732; OTTHUMP00000215877; OTTHUMP00000215878; OTTHUMP00000215879

NCBI Summary for CDC14B

The protein encoded by this gene is a member of the dual specificity protein tyrosine phosphatase family. This protein is highly similar to Saccharomyces cerevisiae Cdc14, a protein tyrosine phosphatase involved in the exit of cell mitosis and initiation of DNA replication, which suggests the role in cell cycle control. This protein has been shown to interact with and dephosphorylates tumor suppressor protein p53, and is thought to regulate the function of p53. Alternative splice of this gene results in 3 transcript variants encoding distinct isoforms. [provided by RefSeq]
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UniProt Comments for CDC14B

CDC14B: Dual-specificity phosphatase involved in DNA damage response. Essential regulator of the G2 DNA damage checkpoint: following DNA damage, translocates to the nucleus and dephosphorylates FZR1/CDH1, a key activator of the anaphase promoting complex/cyclosome (APC/C). Dephosphorylation of FZR1/CDH1 activates the APC/C, leading to the ubiquitination of PLK1, preventing entry into mitosis. Preferentially dephosphorylates proteins modified by proline-directed kinases. Belongs to the protein-tyrosine phosphatase family. Non-receptor class CDC14 subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.3.48; Motility/polarity/chemotaxis; Nucleolus; EC 3.1.3.16; Protein phosphatase, dual-specificity

Chromosomal Location of Human Ortholog: 9q22.3

Cellular Component: nucleoplasm; nuclear membrane; nucleolus; nucleus

Molecular Function: protein binding; protein tyrosine/serine/threonine phosphatase activity; protein tyrosine phosphatase activity; protein serine/threonine phosphatase activity

Biological Process: anaphase-promoting complex activation; protein amino acid dephosphorylation; G2/M transition DNA damage checkpoint; DNA repair
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Research Articles on CDC14B

1. human HCT116, and human telomerase reverse transcription-immortalized retinal pigment epithelial cells deleted for Cdc14B are DNA damage checkpoint proficient and arrest efficiently in G2 in response to irradiation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CDC14B antibody

 Reference Product  PubMed Publications
 SIRT2 antibody  >2 publications with CDC14B and SIRT2

Pathways associated with anti-CDC14B antibody

 Products by Pathway  Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 PLK1 Signaling Events Pathway antibodies  PLK1 Signaling Events Pathway Diagram

Diseases associated with anti-CDC14B antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >5 publications with CDC14B and Neoplasms
 Carcinoma Antibodies  >3 publications with CDC14B and Carcinoma
 Liver Diseases Antibodies  >2 publications with CDC14B and Liver Diseases
 Lymphoma, B-Cell Antibodies  >1 publications with CDC14B and Lymphoma, B-Cell
 Adenocarcinoma Antibodies  >1 publications with CDC14B and Adenocarcinoma
 Carcinoma, Squamous Cell Antibodies  >1 publications with CDC14B and Carcinoma, Squamous Cell
 Urogenital Neoplasms Antibodies  >1 publications with CDC14B and Urogenital Neoplasms
 Nervous System Neoplasms Antibodies  >1 publications with CDC14B and Nervous System Neoplasms
 Liver Neoplasms Antibodies  >1 publications with CDC14B and Liver Neoplasms
 Liver Neoplasms, Experimental Antibodies  >1 publications with CDC14B and Liver Neoplasms, Experimental

Organs/Tissues associated with anti-CDC14B antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >2 publications with CDC14B and Liver
 Kidney Antibodies  >2 publications with CDC14B and Kidney
 Vascular Antibodies  >1 publications with CDC14B and Vascular
 Skin Antibodies  >1 publications with CDC14B and Skin
 Testis Antibodies  >1 publications with CDC14B and Testis
 Brain Antibodies  >1 publications with CDC14B and Brain
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