Goat Anti-TNR Polyclonal Antibody – Cat# MBS614768

Tenascin R in Human Brainstem. Tenascin R was detected in immersion fixed paraffin-embedded sections of human brainstem (medulla) using 1.7ug/ml MBS614768 overnight at 4°C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue).

Restrictin (TNR) Antibody – Goat Polyclonal

Name: TNR Antibody; Polyclonal; Goat; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS614768
Price: 1120 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS614768

Tenascin R is a 180 kD, 1327 amino acid, extracellular matrix glycoprotein belonging to the Tenascin family of adhesion proteins. Tenascin R is selectively expressed in the central nervous system, affecting neurite outgrowth and synaptic functions. Tenascin R… More details

$1120 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Restrictin (TNR), Polyclonal Antibody

Full Product Name

Restrictin (Janusin, Tenascin R)

Product Gene Name

anti-TNR antibody

Product Synonym Names

Anti -Restrictin (Janusin, Tenascin R)

Clonality

Polyclonal

Isotype

IgG

Host

Goat

Species Reactivity

Human

Form/Format

Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.5ml is used, the concentration will be 0.2mg/ml.

Purity/Purification

Affinity Purified
Purified by human Tenascin R affinity chromatography.

Specificity

Selected for its ability to recognize human Tenascin R in the applications listed. In direct ELISAs and Western Blots, this antibody shows less than 1% crossreactivity with rhTenascin C.

Preparation and Storage

Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

148,280 Da

Chromosome Location

Chromosome: 8; NC_006095.2 (7319619..7369216)

3D Structure

ModBase 3D Structure for Q00546

Immunogen

NS0-derved rhTenascin R isoform 1

Product Categories/Family for anti-TNR antibody

Antibodies; Abs to Extracellular Matrix Proteins

Applications Tested/Suitable for anti-TNR antibody

ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)

Application Notes for anti-TNR antibody

Western Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect human Tenascin R. The detection limit for rhTenascin R is approximately 2 ng/lane and 20 ng/lane under non-reducing and reducing conditions, respectively., Immunohistochemistry: This antibody will detect human Tenascin R in cells and tissues. The working dilution is 2-5ug/ml., Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect human Tenascin R. The detection limit for rhTenascin R is approximately 0.3 ng/well., Optimal dilutions should be determined by each laboratory for each application.

Other Notes

Small volumes of anti-TNR antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TNR purchase

MBS614768 is an antibody targeting TNR. Also known as Restrictin (Janusin, Tenascin R). Reactive in Human. Host: Goat.

Additional Details

NCBI/Uniprot data below describe general gene information for TNR. It may not necessarily be applicable to this product.

NCBI GI #

63614

NCBI GeneID

396213

UniProt Primary Accession #

Q00546

UniProt Related Accession #

Q00546

NCBI Official Full Name

restrictin-precursor

NCBI Official Synonym Full Names

tenascin R (restrictin, janusin)

NCBI Official Symbol

TNR

NCBI Official Synonym Symbols

TNR

UniProt Protein Name

Tenascin-R

UniProt Synonym Protein Names

Restrictin

Protein Family

Tenascin

UniProt Gene Name

TNR

UniProt Entry Name

TENR_CHICK

NCBI Protein Information

tenascin-R; TN-R; restrictin

UniProt Comments for TNR

Function: Neural extracellular matrix (ECM) protein involved in interactions with different cells and matrix components. Involved in cell attachment and neurite formation. Interaction with CNTN1 enhances the neurite outgrowth. Ref.2

Subunit structure: Forms homodimers and homotrimers. Interacts with CNTN1, NFASC and CSPG5. Ref.1 Ref.2 Ref.3 Ref.4 Ref.5

Subcellular location: Secreted › extracellular space › extracellular matrix. Note: May be attach to the cell surface of neural cells.

Tissue specificity: Brain specific. Ref.1

Developmental stage: Expression weakly detectable at E6 embryo, reaches a maximum at E16 and declines in the adult. Ref.1

Domain: The N-terminus cysteine-rich segment may mediate the formation of oligomers. The fibronectin type-III 2-3 mediate the binding to contactin 1. The fibronectin type-III 9 mediates the cell attachment. The fibronectin type-III 2-5 mediate NFASC binding. The fibrinogen C-terminal domain mediates interaction with CSPG5. Ref.5

Sequence similarities: Belongs to the tenascin family.Contains 5 EGF-like domains.Contains 1 fibrinogen C-terminal domain.Contains 9 fibronectin type-III domains.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-TNR antibody

Products by Pathway	Pathway Diagram
ECM-receptor Interaction Pathway antibodies	ECM-receptor Interaction Pathway Diagram
ECM-receptor Interaction Pathway antibodies	ECM-receptor Interaction Pathway Diagram
Focal Adhesion Pathway antibodies	Focal Adhesion Pathway Diagram
Focal Adhesion Pathway antibodies	Focal Adhesion Pathway Diagram

Diseases associated with anti-TNR antibody

Disease Name	Pubmed Publications
Liver Neoplasms Antibodies	>6 publications with TNR and Liver Neoplasms
Carcinoma, Hepatocellular Antibodies	>4 publications with TNR and Carcinoma, Hepatocellular
Female Urogenital Diseases Antibodies	>2 publications with TNR and Female Urogenital Diseases
Spinal Cord Injuries Antibodies	>2 publications with TNR and Spinal Cord Injuries
Congenital Abnormalities Antibodies	>2 publications with TNR and Congenital Abnormalities
Inflammation Antibodies	>1 publications with TNR and Inflammation
Prostatic Neoplasms Antibodies	>1 publications with TNR and Prostatic Neoplasms
Demyelinating Diseases Antibodies	>1 publications with TNR and Demyelinating Diseases

Organs/Tissues associated with anti-TNR antibody

Organ/Tissue Name	Pubmed Publications
Head Antibodies	>8 publications with TNR and Head