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MAP4K5 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6148115

May play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway. More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MAP4K5, Monoclonal Antibody
Full Product Name
MAP4K5 (Mitogen-activated Protein Kinase Kinase Kinase Kinase 5, Kinase Homologous to SPS1/STE20, KHS, MAPK/ERK Kinase Kinase Kinase 5, MEK Kinase Kinase 5, MEKKK 5) (FITC)
Product Gene Name
anti-MAP4K5 antibody
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
3H3
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human MAP4K5.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
95,040 Da
Immunogen
Partial recombinant corresponding to aa361-470 from MAP4K5 (AAH36013) with GST tag. MW of the GST tag alone is 26kD.
Conjugate
FITC

Product Categories/Family for anti-MAP4K5 antibody

Antibodies; Protein Kinases
Applications Tested/Suitable for anti-MAP4K5 antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-MAP4K5 antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-MAP4K5 antibody

Western Blot detection against Immunogen (38.1kD).
Mouse Anti-MAP4K5 Monoclonal Antibody – Cat# MBS6148115

Western Blot (WB) of anti-MAP4K5 antibody

Western Blot analysis of MAP4K5 expression in transfected 293T cell line by MAP4K5 monoclonal antibody. Lane 1: MAP4K5 transfected lysate (95.04kD). Lane 2: Non-transfected lysate.
Mouse Anti-MAP4K5 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6148115

Testing Data of anti-MAP4K5 antibody

Detection limit for recombinant GST tagged MAP4K5 is ~0.03ng/ml as a capture antibody.
Mouse Anti-MAP4K5 Monoclonal Antibody, Testing Data – Cat# MBS6148115

Western Blot (WB) of anti-MAP4K5 antibody

Western blot analysis of MAP4K5 over-expressed 293 cell line, cotransfected with MAP4K5 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with MAP4K5 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.
Mouse Anti-MAP4K5 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6148115

Other Notes

Small volumes of anti-MAP4K5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MAP4K5 purchase

MBS6148115 is an antibody targeting MAP4K5. Also known as MAP4K5 (Mitogen-activated Protein Kinase Kinase Kinase Kinase 5, Kinase Homologous to SPS1/STE20, KHS, MAPK/ERK Kinase Kinase Kinase 5, MEK Kinase Kinase 5, MEKKK 5) (FITC). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for MAP4K5. It may not necessarily be applicable to this product.

NCBI GI #
23273902
NCBI GeneID
11183
NCBI Accession #
BC036013
NCBI Related Accession #
AAH36013
UniProt Secondary Accession #
Q8IYF6
UniProt Related Accession #
Q9Y4K4
NCBI Official Full Name
Homo sapiens mitogen-activated protein kinase kinase kinase kinase 5, mRNA
NCBI Official Synonym Full Names
mitogen-activated protein kinase kinase kinase kinase 5
NCBI Official Symbol
MAP4K5
NCBI Official Synonym Symbols
KHS; GCKR; KHS1; MAPKKKK5
Protein Family
Mitogen-activated protein kinase kinase kinase kinase
NCBI Protein Information
mitogen-activated protein kinase kinase kinase kinase 5

NCBI Summary for MAP4K5

This gene encodes a member of the serine/threonine protein kinase family, that is highly similar to yeast SPS1/STE20 kinase. Yeast SPS1/STE20 functions near the beginning of the MAP kinase signal cascades that is essential for yeast pheromone response. This kinase was shown to activate Jun kinase in mammalian cells, which suggested a role in stress response. Two alternatively spliced transcript variants encoding the same protein have been described for this gene. [provided by RefSeq, Jul 2008]
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Research Articles on MAP4K5

1. TNF signaling leads to oligomerization, ubiquitination, and activation of GCKR and activation of the SAPK pathway in a manner that depends upon TRAF2 polyubiquitination and oligomerization and Ubc13 function

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-MAP4K5 antibody

 Products by Pathway  Pathway Diagram
 Insulin Signaling Pathway antibodies  Insulin Signaling Pathway Diagram
 TNF Receptor Signaling Pathway antibodies  TNF Receptor Signaling Pathway Diagram

Diseases associated with anti-MAP4K5 antibody

 Disease Name  Pubmed Publications
 Dyslipidemias Antibodies  >1 publications with MAP4K5 and Dyslipidemias
 Neoplasms Antibodies  >1 publications with MAP4K5 and Neoplasms
 Necrosis Antibodies  >1 publications with MAP4K5 and Necrosis

Organs/Tissues associated with anti-MAP4K5 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >4 publications with MAP4K5 and Blood
 Muscle Antibodies  >1 publications with MAP4K5 and Muscle
 Lung Antibodies  >1 publications with MAP4K5 and Lung
 Brain Antibodies  >1 publications with MAP4K5 and Brain
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