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MARK3 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6148140

Involved in the specific phosphorylation of microtubule-associated proteins for tau, MAP2 and MAP4. Phosphorylates CDC25C on 'Ser-216'. Regulates localization and activity of some histone deacetylases by mediating phosphorylation of HDAC7, promoting… More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MARK3, Monoclonal Antibody
Full Product Name
MARK3 (MAP/Microtubule Affinity-regulating Kinase 3, Cdc25C-associated Protein Kinase 1, cTAK1, C-TAK1, ELKL Motif Kinase 2, EMK2, EMK-2, KP78, Protein Kinase STK10, Ser/Thr Protein Kinase PAR-1, PAR1A, Par-1a, Serine/Threonine-protein Kinase p78) (FITC)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
1A10
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human MARK3.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
74,151 Da
OMIM
602678
3D Structure
ModBase 3D Structure for P27448
Immunogen
Partial recombinant corresponding to aa402-500 from human MARK3 (NP_002367.4) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
HHKVQRSVFSSQKQRRYSDHAGPAIPSVVAYPKRSQTSTADSDLKEDGISSRKSSGSAVGGKGIAPASPMLGNASNPNKADIPERKKSSTVPSSNTASG
Conjugate
FITC

Product Categories/Family for anti-MARK3 antibody

Applications Tested/Suitable for anti-MARK3 antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-MARK3 antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-MARK3 antibody

Western Blot detection against Immunogen (36.63kD).
Mouse Anti-MARK3 Monoclonal Antibody – Cat# MBS6148140

Western Blot (WB) of anti-MARK3 antibody

Western Blot analysis of MARK3 expression in transfected 293T cell line by MARK3 monoclonal antibody. Lane 1: MARK3 transfected lysate (81.4kD). Lane 2: Non-transfected lysate.
Mouse Anti-MARK3 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6148140

Testing Data of anti-MARK3 antibody

Detection limit for recombinant GST tagged MARK3 is ~3ng/ml as a capture antibody.
Mouse Anti-MARK3 Monoclonal Antibody, Testing Data – Cat# MBS6148140

Other Notes

Small volumes of anti-MARK3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MARK3 purchase

MBS6148140 is an antibody targeting MARK3. Also known as MARK3 (MAP/Microtubule Affinity-regulating Kinase 3, Cdc25C-associated Protein Kinase 1, cTAK1, C-TAK1, ELKL Motif Kinase 2, EMK2, EMK-2, KP78, Protein Kinase STK10, Ser/Thr Protein Kinase PAR-1, PAR1A, Par-1a, Serine/Threonine-protein Kinase p78) (FITC). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for MARK3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
MAP/microtubule affinity-regulating kinase 3 isoform c
NCBI Official Synonym Full Names
MAP/microtubule affinity-regulating kinase 3
NCBI Official Symbol
MARK3
NCBI Official Synonym Symbols
KP78; CTAK1; PAR1A; Par-1a
UniProt Protein Name
MAP/microtubule affinity-regulating kinase 3
UniProt Synonym Protein Names
C-TAK1; cTAK1; Cdc25C-associated protein kinase 1; ELKL motif kinase 2; EMK-2; Protein kinase STK10; Ser/Thr protein kinase PAR-1; Par-1a; Serine/threonine-protein kinase p78
UniProt Gene Name
MARK3
UniProt Synonym Gene Names
CTAK1; EMK2; cTAK1; EMK-2; Par-1a
UniProt Entry Name
MARK3_HUMAN
NCBI Protein Information
MAP/microtubule affinity-regulating kinase 3; EMK-2; C-TAK1; ELKL motif kinase 2; protein kinase STK10; ser/Thr protein kinase PAR-1; cdc25C-associated protein kinase 1; serine/threonine-protein kinase p78

NCBI Summary for MARK3

The protein encoded by this gene is activated by phosphorylation and in turn is involved in the phosphorylation of tau proteins MAP2 and MAP4. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

UniProt Comments for MARK3

MARK3: an ubiquitous serine/threonine kinase of the CAMKL family. Phosphorylates Cdc25C on serine 216 and promotes 14-3-3 protein binding. A positive regulator of Wnt signalling. Ubiquitously expressed in human tissues and cell lines. Seven alternatively spliced isoforms have been reported.

Protein type: EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, CAMK; CAMK group; CAMKL family; MARK subfamily

Chromosomal Location of Human Ortholog: 14q32.32

Cellular Component: plasma membrane

Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding

Biological Process: protein amino acid phosphorylation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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