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PBX2 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6148692

MBS6148692 is an antibody targeting PBX2. Also known as PBX2 (G17, Pre-B Cell Leukemia Transcription Factor 2, Homeobox Protein PBX2, Protein G17) (FITC). Reactive in Human. Host: Mouse. More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PBX2, Monoclonal Antibody
Full Product Name
PBX2 (G17, Pre-B Cell Leukemia Transcription Factor 2, Homeobox Protein PBX2, Protein G17) (FITC)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
2E9
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human PBX2.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
46kDa
OMIM
X59842 mRNA
3D Structure
ModBase 3D Structure for P40425
Immunogen
Partial recombinant corresponding to aa351-431 from human PBX2 (NP_002577) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
DMFLGMPGLNGDSYSASQVESLRHSMGPGGYGDNLGGGQMYSPREMRANGSWQEAVTPSSVTSPTEGPGSVHSDTSN
Conjugate
FITC
Applications Tested/Suitable for anti-PBX2 antibody
ELISA (EIA), Immunofluorescence (IF), Western Blot (WB)
Application Notes for anti-PBX2 antibody
IF: 10ug/ml
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-PBX2 antibody

Western Blot detection against Immunogen (34.21kD).
Mouse Anti-PBX2 Monoclonal Antibody – Cat# MBS6148692

Western Blot (WB) of anti-PBX2 antibody

PBX2 monoclonal antibody Western Blot analysis of PBX2 expression in Hela NE.
Mouse Anti-PBX2 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6148692

Western Blot (WB) of anti-PBX2 antibody

Western Blot analysis of PBX2 expression in transfected 293T cell line by PBX2 monoclonal antibody. Lane 1: PBX2 transfected lysate (45.9kD). Lane 2: Non-transfected lysate.
Mouse Anti-PBX2 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6148692

Immunofluorescence (IF) of anti-PBX2 antibody

Immunofluorescence of monoclonal antibody to PBX2 on HeLa cell. [antibody concentration 10ug/ml]
Mouse Anti-PBX2 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS6148692

Western Blot (WB) of anti-PBX2 antibody

Western blot analysis of PBX2 over-expressed 293 cell line, cotransfected with PBX2 Validated Chimera RNAi ((Lane 2) or non-transfected control (Lane 1). Blot probed with PBX2 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.
Mouse Anti-PBX2 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6148692

Other Notes

Small volumes of anti-PBX2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PBX2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
pre-B-cell leukemia transcription factor 2
NCBI Official Synonym Full Names
PBX homeobox 2
NCBI Official Symbol
PBX2
NCBI Official Synonym Symbols
G17; HOX12; PBX2MHC
UniProt Protein Name
Pre-B-cell leukemia transcription factor 2
UniProt Synonym Protein Names
Homeobox protein PBX2; Protein G17
UniProt Gene Name
PBX2
UniProt Synonym Gene Names
G17
NCBI Protein Information
pre-B-cell leukemia transcription factor 2

NCBI Summary for PBX2

This gene encodes a ubiquitously expressed member of the TALE/PBX homeobox family. It was identified by its similarity to a homeobox gene which is involved in t(1;19) translocation in acute pre-B-cell leukemias. This protein is a transcriptional activator which binds to the TLX1 promoter. The gene is located within the major histocompatibility complex (MHC) on chromosome 6. [provided by RefSeq, Jul 2008]
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UniProt Comments for PBX2

PBX2: Transcriptional activator that binds the sequence 5'- ATCAATCAA-3'. Activates transcription of PF4 in complex with MEIS1. Belongs to the TALE/PBX homeobox family.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 6p21.32

Cellular Component: nucleus; transcription factor complex

Molecular Function: chromatin binding; protein binding; transcription factor binding

Biological Process: embryonic limb morphogenesis; positive regulation of transcription from RNA polymerase II promoter; proximal/distal pattern formation; transcription from RNA polymerase II promoter
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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