• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

PRKCI Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6149043

MBS6149043 is an antibody targeting PRKCI. Also known as PRKCI (Protein Kinase C iota Type, Atypical Protein Kinase C-lambda/iota, PRKC-lambda/iota, aPKC-lambda/iota, nPKC-iota, DXS1179E, MGC26534) (FITC). Reactive in Human. Host: Mouse. More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PRKCI, Monoclonal Antibody
Full Product Name
PRKCI (Protein Kinase C iota Type, Atypical Protein Kinase C-lambda/iota, PRKC-lambda/iota, aPKC-lambda/iota, nPKC-iota, DXS1179E, MGC26534) (FITC)
Product Gene Name
anti-PRKCI antibody
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
1G11
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human PRKCI.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
68,262 Da
Immunogen
Full length recombinant corresponding to aa1-588, from PRKCI (AAH22016) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MSHTVAGGGSGDHSHQVRVKAYYRGDIMITHFEPSISFEGLCNEVRDMCSFDNEQLFTMKWIDEEGDPCTVSSQLELEEAFRLYELNKDSELLIHVFPCVPERPGMPCPGEDKSIYRRGARRWRKLYCANGHTFQAKRFNRRAHCAICTDRIWGLGRQGYKCINCKLLVHKKCHKLVTIECGRHSLPQEPVMPMDQSSMHSDHAQTVIPYNPSSHESLDQVGEEKEAMNTRESGKASSSLGLQDFDLLRVIGRGSYAKVLLVRLKKTDRIYAMKVVKKELVNDDEDIDWVQTEKHVFEQASNHPFLVGLHSCFQTESRLFFVIEYVNGGDLMFHMQRQRKLPEEHARFYSAEISLALNYLHERGIIYRDLKLDNVLLDSEGHIKLTDYGMCKEGLRPGDTTSTFCGTPNYIAPEILRGEDYGFSVDWWALGVLMFEMMAGRSPFDIVGSSDNPDQNTEDYLFQVILEKQIRIPRSMSVKAASVLKSFLNKDPKERLGCLPQTGFADIQGHPFFRNVDWDMMEQKQVVPPFKPNISGEFGLDNFDSQFTNERVQLTPDDDDIVRKIDQSEFEGFEYINPLLMSAEECV
Conjugate
FITC

Product Categories/Family for anti-PRKCI antibody

Antibodies; Protein Kinases
Applications Tested/Suitable for anti-PRKCI antibody
ELISA (EIA), Immunofluorescence (IF), Western Blot (WB)
Application Notes for anti-PRKCI antibody
IF: 10ug/ml
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-PRKCI antibody

Western Blot analysis of PRKCI expression in transfected 293T cell line by PRKCI monoclonal antibody. Lane 1: PRKCI transfected lysate (68.3kD). Lane 2: Non-transfected lysate.
Mouse Anti-PRKCI Monoclonal Antibody – Cat# MBS6149043

Immunofluorescence (IF) of anti-PRKCI antibody

Immunofluorescence of monoclonal antibody to PRKCI on HeLa cell. [antibody concentration 10ug/ml]
Mouse Anti-PRKCI Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS6149043

Testing Data of anti-PRKCI antibody

Detection limit for recombinant GST tagged PRKCI is ~3ng/ml as a capture antibody.
Mouse Anti-PRKCI Monoclonal Antibody, Testing Data – Cat# MBS6149043

Western Blot (WB) of anti-PRKCI antibody

Western blot analysis of PRKCI over-expressed 293 cell line, cotransfected with PRKCI Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PRKCI monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.
Mouse Anti-PRKCI Monoclonal Antibody, Western Blot (WB) – Cat# MBS6149043

Other Notes

Small volumes of anti-PRKCI antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PRKCI. It may not necessarily be applicable to this product.

NCBI GI #
34191041
NCBI GeneID
5584
NCBI Accession #
BC022016
NCBI Related Accession #
AAH22016
UniProt Secondary Accession #
Q8WW06; D3DNQ4
UniProt Related Accession #
P41743
NCBI Official Full Name
Homo sapiens protein kinase C, iota, mRNA
NCBI Official Synonym Full Names
protein kinase C iota
NCBI Official Symbol
PRKCI
NCBI Official Synonym Symbols
PKCI; DXS1179E; nPKC-iota
Protein Family
Protein kinase
NCBI Protein Information
protein kinase C iota type

NCBI Summary for PRKCI

This gene encodes a member of the protein kinase C (PKC) family of serine/threonine protein kinases. The PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. This protein kinase is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. There is a single exon pseudogene mapped on chromosome X. [provided by RefSeq, Jul 2008]
Show more

Research Articles on PRKCI

1. the results revealed that PRKCI rs546950 variant decreased the risk of prostate cancer in an Iranian population

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-PRKCI antibody

 Products by Pathway  Pathway Diagram
 Cell Junction Organization Pathway antibodies  Cell Junction Organization Pathway Diagram
 Cell-Cell Communication Pathway antibodies  Cell-Cell Communication Pathway Diagram
 Cell-cell Junction Organization Pathway antibodies  Cell-cell Junction Organization Pathway Diagram
 EGFR1 Signaling Pathway antibodies  EGFR1 Signaling Pathway Diagram
 Endocytosis Pathway antibodies  Endocytosis Pathway Diagram
 Endocytosis Pathway antibodies  Endocytosis Pathway Diagram
 G Protein Signaling Pathways antibodies  G Protein Signaling Pathways Diagram
 Hippo Signaling Pathway antibodies  Hippo Signaling Pathway Diagram
 Hippo Signaling Pathway antibodies  Hippo Signaling Pathway Diagram
 IL-4 Signaling Pathway antibodies  IL-4 Signaling Pathway Diagram

Diseases associated with anti-PRKCI antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >8 publications with PRKCI and Neoplasms
 Cell Transformation, Neoplastic Antibodies  >2 publications with PRKCI and Cell Transformation, Neoplastic
 Neoplasm Metastasis Antibodies  >2 publications with PRKCI and Neoplasm Metastasis
 Breast Neoplasms Antibodies  >1 publications with PRKCI and Breast Neoplasms
 Hyperplasia Antibodies  >1 publications with PRKCI and Hyperplasia
 Kidney Diseases Antibodies  >1 publications with PRKCI and Kidney Diseases
 Inflammation Antibodies  >1 publications with PRKCI and Inflammation

Organs/Tissues associated with anti-PRKCI antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >4 publications with PRKCI and Blood
 Brain Antibodies  >3 publications with PRKCI and Brain
 Intestine Antibodies  >2 publications with PRKCI and Intestine
 Lung Antibodies  >2 publications with PRKCI and Lung
 Prostate Antibodies  >2 publications with PRKCI and Prostate
 Muscle Antibodies  >2 publications with PRKCI and Muscle
 Heart Antibodies  >1 publications with PRKCI and Heart
 Spinal Cord Antibodies  >1 publications with PRKCI and Spinal Cord
 Ovary Antibodies  >1 publications with PRKCI and Ovary
 Kidney Antibodies  >1 publications with PRKCI and Kidney
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000