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PRKCZ Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6149045

Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent,… More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
PRKCZ, Monoclonal Antibody
Full Product Name
PRKCZ (Protein Kinase C zeta Type, nPKC-zeta, PKC2) (FITC)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
2D1
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human PRKCZ.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
56,136 Da
Immunogen
Partial recombinant corresponding to aa165-255, from human PRKCZ (AAH08058) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
KLLVHKRCHGLVPLTCRKHMDSVMPSQEPPVDDKNEDADLPSEETDGIAYISSSRKHDSIKDDSEDLKPVIDGMDGIKISQGLGLQDFDLI
Conjugate
FITC

Product Categories/Family for anti-PRKCZ antibody

Applications Tested/Suitable for anti-PRKCZ antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-PRKCZ antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-PRKCZ antibody

Western Blot analysis of PRKCZ expression in transfected 293T cell line by PRKCZ monoclonal antibody. Lane 1: PRKCZ transfected lysate (67.7kD). Lane 2: Non-transfected lysate.
Mouse Anti-PRKCZ Monoclonal Antibody – Cat# MBS6149045

Testing Data of anti-PRKCZ antibody

Detection limit for recombinant GST tagged PRKCZ is ~0.1ng/ml as a capture antibody.
Mouse Anti-PRKCZ Monoclonal Antibody, Testing Data – Cat# MBS6149045

Western Blot (WB) of anti-PRKCZ antibody

Western blot analysis of PRKCZ over-expressed 293 cell line, cotransfected with PRKCZ Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PRKCZ monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.
Mouse Anti-PRKCZ Monoclonal Antibody, Western Blot (WB) – Cat# MBS6149045

Other Notes

Small volumes of anti-PRKCZ antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PRKCZ purchase

MBS6149045 is an antibody targeting PRKCZ. Also known as PRKCZ (Protein Kinase C zeta Type, nPKC-zeta, PKC2) (FITC). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for PRKCZ. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Homo sapiens protein kinase C, zeta, mRNA
NCBI Official Synonym Full Names
protein kinase C zeta
NCBI Official Symbol
PRKCZ
NCBI Official Synonym Symbols
PKC2; PKC-ZETA
Protein Family
NCBI Protein Information
protein kinase C zeta type

NCBI Summary for PRKCZ

Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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