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PRKRA Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6149049

MBS6149049 is an antibody targeting PRKRA. Also known as PRKRA (Interferon-inducible Double Stranded RNA-dependent Protein Kinase Activator A, PKR-associated Protein X, PACT, RAX, PKR-associating Protein X, Protein Activator of the Interferon-induced Protein… More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PRKRA, Monoclonal Antibody
Full Product Name
PRKRA (Interferon-inducible Double Stranded RNA-dependent Protein Kinase Activator A, PKR-associated Protein X, PACT, RAX, PKR-associating Protein X, Protein Activator of the Interferon-induced Protein Kinase, Protein Kinase, Interferon-inducible Double S
Product Gene Name
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
1B9-1A7
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human PRKRA.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
31,635 Da
Immunogen
Full length recombinant corresponding to aa1-313 from PRKRA (AAH09470) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MSQSRHRAEAPPLEREDSGTFSLGKMITAKPGKTPIQVLHEYGMKTKNIPVYECERSDVQIHVPTFTFRVTVGDITCTGEGTSKKLAKHRAAEAAINILKANASICFAVPDPLMPDPSKQPKNQLNPIGSLQELAIHHGWRLPEYTLSQEGGPAHKREYTTICRLESFMETGKGASKKQAKRNAAEKFLAKFSNISPENHISLTNVVGHSLGCTWHSLRNSPGEKINLLKRSLLSIPNTDYIQLLSEIAKEQGFN
Conjugate
FITC

Product Categories/Family for anti-PRKRA antibody

Applications Tested/Suitable for anti-PRKRA antibody
ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Immunoprecipitation (IP), Western Blot (WB)
Application Notes for anti-PRKRA antibody
IHC-P: 5ug/ml
Applications are based on unconjugated antibody.

Testing Data of anti-PRKRA antibody

Immunoperoxidase of monoclonal antibody to PRKRA on formalin-fixed paraffin-embedded human squamous cell carcinoma. [antibody concentration 5ug/ml].
Mouse Anti-PRKRA Monoclonal Antibody – Cat# MBS6149049

Immunohistochemistry (IHC) of anti-PRKRA antibody

Immunoperoxidase of monoclonal antibody to PRKRA on formalin-fixed paraffin-embedded human tonsil tissue. [antibody concentration 5ug/ml].
Mouse Anti-PRKRA Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6149049

Immunoprecipitation (IP) of anti-PRKRA antibody

Immunoprecipitation of PRKRA transfected lysate using PRKRA monoclonal antibody and Protein A Magnetic Bead and immunoblotted with PRKRA rabbit polyclonal antibody.
Mouse Anti-PRKRA Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS6149049

Western Blot (WB) of anti-PRKRA antibody

Western Blot analysis of PRKRA expression in transfected 293T cell line by PRKRA monoclonal antibody Lane 1: PRKRA transfected lysate (34.4kD). Lane 2: Non-transfected lysate.
Mouse Anti-PRKRA Monoclonal Antibody, Western Blot (WB) – Cat# MBS6149049

Western Blot (WB) of anti-PRKRA antibody

Western Blot detection against Immunogen (60.54kD).
Mouse Anti-PRKRA Monoclonal Antibody, Western Blot (WB) – Cat# MBS6149049

Other Notes

Small volumes of anti-PRKRA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PRKRA. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Homo sapiens protein kinase, interferon-inducible double stranded RNA dependent activator, mRNA
NCBI Official Synonym Full Names
protein activator of interferon induced protein kinase EIF2AK2
NCBI Official Symbol
PRKRA
NCBI Official Synonym Symbols
RAX; PACT; DYT16; HSD14
NCBI Protein Information
interferon-inducible double-stranded RNA-dependent protein kinase activator A

NCBI Summary for PRKRA

This gene encodes a protein kinase activated by double-stranded RNA which mediates the effects of interferon in response to viral infection. Mutations in this gene have been associated with dystonia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2008]

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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