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PRPH Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6149072

MBS6149072 is an antibody targeting PRPH. Also known as PRPH (Peripherin, Neurofilament 4, NEF4, PRPH1) (FITC). Reactive in Human. Host: Mouse. More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PRPH, Monoclonal Antibody
Full Product Name
PRPH (Peripherin, Neurofilament 4, NEF4, PRPH1) (FITC)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
3B3
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human PRPH.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
53,779 Da
OMIM
170710
3D Structure
ModBase 3D Structure for P41219
Immunogen
Partial recombinant protein corresponding to aa374-470 of human PRPH with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
RHLREYQELLNVKMALDIEIATYRKLLEGEESRISVPVHSFASLNIKTTVPEVEPPQDSHSRKTVLIKTIETRNGEVVTESQKEQRSELDKSSAHSY
Conjugate
FITC

Product Categories/Family for anti-PRPH antibody

Applications Tested/Suitable for anti-PRPH antibody
FLISA, Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes for anti-PRPH antibody
Applications are based on unconjugated antibody.

Testing Data of anti-PRPH antibody

Detection limit for recombinant GST tagged PRPH is 1ng/ml using 131884 as a capture antibody.
Mouse Anti-PRPH Monoclonal Antibody – Cat# MBS6149072

Western Blot (WB) of anti-PRPH antibody

Western Blot analysis of PRPH expression in IMR-32 using 131884.
Mouse Anti-PRPH Monoclonal Antibody, Western Blot (WB) – Cat# MBS6149072

Immunohistochemistry (IHC) of anti-PRPH antibody

Immunoperoxidase of formalin-fixed paraffin-embedded human placenta using 131884 (3ug/ml).
Mouse Anti-PRPH Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6149072

Immunofluorescence (IF) of anti-PRPH antibody

Immunofluorescence of on HeLa cell using 131884 (10ug/ml).
Mouse Anti-PRPH Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS6149072

Other Notes

Small volumes of anti-PRPH antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PRPH. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
peripherin
NCBI Official Synonym Full Names
peripherin
NCBI Official Symbol
PRPH
NCBI Official Synonym Symbols
NEF4; PRPH1
UniProt Protein Name
Peripherin
UniProt Synonym Protein Names
Neurofilament 4
Protein Family
UniProt Gene Name
PRPH
UniProt Synonym Gene Names
NEF4; PRPH1
UniProt Entry Name
PERI_HUMAN
NCBI Protein Information
peripherin; neurofilament 4 (57kD)

UniProt Comments for PRPH

peripherin: Class-III neuronal intermediate filament protein. Belongs to the intermediate filament family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 12q12-q13

Cellular Component: membrane; type III intermediate filament; neurofilament; intermediate filament

Molecular Function: structural molecule activity

Biological Process: intermediate filament cytoskeleton organization and biogenesis

Disease: Amyotrophic Lateral Sclerosis 1
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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