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Kip1 P57 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS614982

MBS614982 is an antibody targeting Kip1 p57. Also known as Kip1 p57 (cdk Inhibitor, Cyclin Dependent Kinase Inhibitor). Reactive in Human, Rabbit, Rat. Host: Rabbit. More details

$995 / 0.2 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Kip1 p57, Polyclonal Antibody
Full Product Name
Kip1 p57 (cdk Inhibitor, Cyclin Dependent Kinase Inhibitor)
Product Synonym Names
Anti -Kip1 p57 (cdk Inhibitor, Cyclin Dependent Kinase Inhibitor)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Rabbit, Rat
Form/Format
Supplied as a solution in 0.1M Tris-glycine, pH 7.4.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Species crossreactivity: Rabbit, human and rat.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C or colder. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
An 18 amino acid synthetic peptide ([C]GSVEQTPKKPGLRRRQT) linked to KLH was used to immunize rabbits. The human Kip1 sequence used (amino acids 182-198) shares 13/17 amino acids with murine Kip1. No other known proteins share more than 8 amino acids with the sequence.
Applications Tested/Suitable for anti-Kip1 p57 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes for anti-Kip1 p57 antibody
Suitable for use in Western Blotting, Immunoprecipitation, and Immunohistochemistry.
Dilution: Western Blotting: 1ug/ml detected Kip1 (~27kD) in a HeLa nuclear extract and a rabbit brain nuclear extract.
Immunohistochemistry: 5ug/ml showed positive immunostaining for Kip1 in formalin-fixed, paraffin embedded rat brain tissue.
Immunoprecipitation: 4ug immunoprecipitated Kip1 from a HeLa nuclear extract and a rabbit brain nuclear extract.

Western Blot (WB) of anti-Kip1 p57 antibody

WB: HeLa nuclear extract was probed with anti-Kip1 (p27) (0.5ug/ml). Arrow indicates Kip1 (p27).
Rabbit Anti-Kip1 P57 Polyclonal Antibody – Cat# MBS614982

Other Notes

Small volumes of anti-Kip1 p57 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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