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Thioredoxin (TRDX) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6150301

MBS6150301 is an antibody targeting TRDX. Also known as Thioredoxin (TRDX, Trx, TRX1, TXN, ATL-derived Factor, ADF, Surface-associated Sulphydryl Protein, SASP) (FITC). Reactive in Human. Host: Mouse. More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Thioredoxin (TRDX), Monoclonal Antibody
Full Product Name
Thioredoxin (TRDX, Trx, TRX1, TXN, ATL-derived Factor, ADF, Surface-associated Sulphydryl Protein, SASP) (FITC)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
2A7
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human TXN.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
Calculated Molecular Weight: 12kDa
Observed Molecular Weight: 12kDa
OMIM
187700
3D Structure
ModBase 3D Structure for P10599
Immunogen
Full length recombinant corresponding to aa1-105 from human TXN (AAH03377) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MVKQIESKTAFQEALDAAGDKLVVVDFSATWCGPCKMIKPFFHSLSEKYSNVIFLEVDVDDCQDVASECEVKCMPTFQFFKKGQKVGEFSGANKEKLEATINELV
Conjugate
FITC

Product Categories/Family for anti-TRDX antibody

Applications Tested/Suitable for anti-TRDX antibody
ELISA (EIA), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB)
Application Notes for anti-TRDX antibody
IF: 10ug/ml
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-TRDX antibody

TXN monoclonal antibody Western Blot analysis of TXN expression in HeLa.
Mouse Anti-TRDX Monoclonal Antibody – Cat# MBS6150301

Western Blot (WB) of anti-TRDX antibody

Western Blot analysis of TXN expression in transfected 293T cell line by TXN monoclonal antibody. Lane 1: TXN transfected lysate (11.7kD). Lane 2: Non-transfected lysate.
Mouse Anti-TRDX Monoclonal Antibody, Western Blot (WB) – Cat# MBS6150301

Immunofluorescence (IF) of anti-TRDX antibody

Immunofluorescence of monoclonal antibody to TXN on HeLa cell. [antibody concentration 10ug/ml]
Mouse Anti-TRDX Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS6150301

Immunoprecipitation (IP) of anti-TRDX antibody

Immunoprecipitation of TXN transfected lysate using TXN monoclonal antibody and Protein A Magnetic Bead and immunoblotted with TXN rabbit polyclonal antibody.
Mouse Anti-TRDX Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS6150301

Testing Data of anti-TRDX antibody

Detection limit for recombinant GST tagged TXN is ~0.03ng/ml as a capture antibody.
Mouse Anti-TRDX Monoclonal Antibody, Testing Data – Cat# MBS6150301

Other Notes

Small volumes of anti-TRDX antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for TRDX. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Homo sapiens thioredoxin, mRNA
NCBI Official Synonym Full Names
thioredoxin
NCBI Official Symbol
TXN
NCBI Official Synonym Symbols
TRX; TRDX; TRX1
UniProt Protein Name
Thioredoxin
UniProt Synonym Protein Names
ATL-derived factor; ADF; Surface-associated sulphydryl protein
Protein Family
UniProt Gene Name
TXN
UniProt Synonym Gene Names
TRDX; TRX; TRX1; Trx; ADF; SASP
UniProt Entry Name
THIO_HUMAN
NCBI Protein Information
thioredoxin

NCBI Summary for TRDX

The protein encoded by this gene acts as a homodimer and is involved in many redox reactions. The encoded protein is active in the reversible S-nitrosylation of cysteines in certain proteins, which is part of the response to intracellular nitric oxide. This protein is found in the cytoplasm. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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